IP Library › Granted Patent US 12,559,740
Granted Patent B2
US 12,559,740 · App. 17/917,859 · Granted Feb 24, 2026

Process for the direct enzymatic conversion of amino sugars; enzyme and compositions for use in the process

Inventors: Josef Voglmeir (Jiangsu, CN); Li Liu (Jiangsu, CN)
Assignee: GLYCOMICS AND GLYCAN BIOENGINEERING RESEARCH CENTER (GGBRC), NANJING AGRICULTURAL UNIVERSITY
C12N9/78C12P3/00C12P19/02C12Y305/99006
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Quick Facts
Patent No.
US 12,559,740
App. No.
17/917,859
Granted
Feb 24, 2026
Kind
B2
Abstract

A process for converting amino sugar to fructose and ammonia using one enzyme. Also provided are compositions and enzymes for converting amino sugar to fructose and ammonia.

Claims (21)

1 . A process for converting amino sugar to fructose and ammonia, the steps of which consist of:

optionally hydrolyzing a biomaterial comprising amino sugars; and

converting the amino sugars to fructose and ammonia via a single type of enzyme consisting of glucosamine-6-phosphate deaminase (GPDA), wherein the enzyme comprises the amino acid sequence selected from the group consisting of: SEQ ID NO:33 and SEQ ID NO:35; and

wherein the amino sugars are glucosamine.

2 . The process according to claim 1 , wherein the glucosamine-6-phosphate deaminase (GPDA enzyme) is an isolated polypeptide or a whole-cell biocatalyst.

3 . The process according to claim 1 , wherein at least 10% of the amino sugars are converted to fructose and ammonia after 24 hours of enzymatic conversion.

4 . The process according to claim 1 , wherein the process is a one-step process.

5 . The process according to claim 1 , wherein the amino sugars are obtained from hydrolyzing chitin- or chitosan-containing biomaterials.

6 . The process according to claim 1 , wherein in said step the glucosamine is contacted with the GPDA in the presence of water.

7 . The process according to claim 1 , wherein the enzyme is immobilized.

8 . The process according to claim 1 , wherein the biomaterial comprises amino polysaccharides.

9 . The process according to claim 1 , wherein the converting is a deamination-isomerization reaction.

10 . An enzyme for converting glucosamine to fructose and ammonia, wherein the enzyme comprises the amino acid sequence selected from the group consisting of: SEQ ID NO:33 and SEQ ID NO:35.

11 . A composition for converting amino sugar to fructose and ammonia, comprising:

water;

an amino sugar;

glucosamine-6-phosphate deaminase (GPDA) comprising the amino acid sequence selected from the group consisting of: SEQ ID NO:33 and SEQ ID NO:35;

wherein the amino sugar is glucosamine.

12 . The process according to claim 3 , wherein at least 15% of the amino sugars are converted to fructose and ammonia after 24 hours of enzymatic conversion.

13 . The process according to claim 5 , wherein the chitin- or chitosan-containing biomaterials comprise shellfish exoskeletons, insects, and fungi.

14 . The process according to claim 8 , wherein the biomaterial comprises chitin or chitosan.

Assignments (2)
CHANGE OF NAME Recorded Nov 18, 2024
From: VOGLMEIR, JOSEF; LIU, LI
To: GLYCOMICS AND GLYCAN BIOENGINEERING RESEARCH CENTER (GGBRC), NANJING AGRICULTURAL UNIVERSITY
Reel/Frame 069379/0927 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 22, 2023
From: VOGLMEIR, JOSEF; LIU, LI
To: GLYCOMICS AND GLYCAN BIOENGINEERING RESEARCH CENTER (GGBRC), COLLEGE OF FOOD SCIENCE AND TECHNOLOGY, NANJING AGRICULTURAL UNIVERSITY
Reel/Frame 062772/0401 →
Continuity (1)
Related Publication 20240392278A1 · Nov 28, 2024
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