Adenovirus immunoassay method and adenovirus immunoassay instrument
An object of the present invention is to provide: a monoclonal antibody that makes it possible that adenovirus contained in a test specimen is detected and measured rapidly, simply, and with high-sensitivity; and an immunoassay for adenovirus and an immunoassay device therefor, for both of which the monoclonal antibody is used. The present invention provides: a monoclonal antibody or an antigen-binding fragment thereof, including heavy chains CDR1 to CDR3 as shown in the following (a) to (c) and a light chain CDR1 as shown in the following (d); and an immunoassay and an immunoassay device, for both of which the monoclonal antibody or the antigen-binding fragment thereof is used; (a) a heavy chain CDR1 composed of an amino acid sequence containing NY; (b) a heavy chain CDR2 composed of an amino acid sequence containing SN; (c) a heavy chain CDR3 composed of an amino acid sequence containing SYY and DY; and (d) a light chain CDR1 composed of an amino acid sequence containing NG.
1 . A monoclonal antibody or an antigen-binding fragment, thereof, comprising heavy chains CDR1 to CDR3 as shown in the following (a-1) to (c-1) and light chains CDR1 to CDR3 as shown in the following (d-1) to (f-1):
(a-1) a heavy chain CDR1 consisting of an amino acid sequence of SEQ ID NO: 1;
(b-1) a heavy chain CDR2 consisting of an amino acid sequence of SEQ ID NO: 2;
(c-1) a heavy chain CDR3 consisting of an amino acid sequence of SEQ ID NO: 3;
(d-1) a light chain CDR1 consisting of an amino acid sequence of SEQ ID NO: 4;
(e-1) a light chain CDR2 consisting of an amino acid sequence of SEQ ID NO: 5; and
(f-1) a light chain CDR3 consisting of an amino acid sequence of SEQ ID NO: 6.
2 . A monoclonal antibody or an antigen-binding fragment thereof, comprising heavy chains CDR1 to CDR3 as shown in the following (a-2) to (c-2) and light chains CDR1 to CDR3 as shown in the following (d-2) to (f-2):
(a-2) a heavy chain CDR1 consisting of an amino acid sequence of SEQ ID NO: 7;
(b-2) a heavy chain CDR2 consisting of an amino acid sequence of SEQ ID NO: 8;
(c-2) a heavy chain CDR3 consisting of an amino acid sequence of SEQ ID NO: 9;
(d-2) a light chain CDR1 consisting of an amino acid sequence of SEQ ID NO: 10;
(e-2) a light chain CDR2 consisting of an amino acid sequence of SEQ ID NO: 11; and
(f-2) a light chain CDR3 consisting of an amino acid sequence containing of SEQ ID NO: 12.
3 . An immunoassay method for detecting an adenovirus, comprising performing the immunoassay of the adenovirus by using an antigen-antibody reaction between the monoclonal antibody or the antigen-binding fragment thereof according to claim 1 and adenovirus in a sample.
4 . The immunoassay method according to claim 3 , wherein the immunoassay is a sandwich method, and the monoclonal antibody or the antigen-binding fragment thereof is immobilized on a solid phase and/or is labeled for use in a sandwich immunoassay.
5 . An immunoassay device for adenovirus, comprising the monoclonal antibody or the antigen-binding fragment thereof according to claim 1 .
6 . An immunoassay method for detecting an adenovirus, comprising performing the immunoassay of the adenovirus by using an antigen-antibody reaction between the monoclonal antibody or the antigen-binding fragment thereof according to claim 2 and adenovirus in a sample.