IP Library › Granted Patent US 12,644,139
Granted Patent B2
US 12,644,139 · App. 17/926,487 · Granted Jun 2, 2026

Cells and methods for producing methyl ketones

Inventors: Brian F. Pfleger (Madison, WI); Qiang Yan (Madison, WI); Trevor R. Simmons (Austin, TX); Nestor Hernandez-Lozada (Jena, DE)
Assignee: Wisconsin Alumni Research Foundation
C12P7/26C12N9/0006C12N9/16C12N9/93C12N15/52C12N15/74C12N2330/50C12Y101/01035C12Y301/0202C12Y301/02021C12Y402/01017C12Y602/01001
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Quick Facts
Patent No.
US 12,644,139
App. No.
17/926,487
Granted
Jun 2, 2026
Kind
B2
Abstract

Recombinant cells and methods for producing methyl ketones, such as medium-chain methyl ketones. The recombinant cells include recombinant acyl-ACP thioesterase genes, recombinant β-ketoacyl-CoA thioesterase genes, and recombinant acyl-CoA synthetase genes, in addition to other modifications. The methods include culturing the recombinant cells to produce the methyl ketones and isolating the produced methyl ketones.

Claims (47)

1 . A recombinant cell for producing methyl ketones comprising:

a recombinant β-ketoacyl-CoA thioesterase gene;

a recombinant acyl-ACP thioesterase gene;

a recombinant acyl-CoA synthetase gene;

a recombinant 3-ketoacyl-CoA thiolase gene; and

a functional deletion of at least one of a native enoyl-CoA hydratase gene and a native 3-hydroxyacyl-CoA dehydrogenase gene.

2 . The recombinant cell of claim 1 , further comprising a functional deletion of a native fadR gene or a homolog thereof.

3 . The recombinant cell of claim 1 , further comprising a functional deletion of a native acyl-CoA dehydrogenase gene.

4 . The recombinant cell of claim 1 , wherein the recombinant cell exhibits enhanced production of methyl ketone with respect to a corresponding native cell.

5 . The recombinant cell of claim 4 , wherein the methyl ketone comprises medium-chain methyl ketone.

6 . The recombinant cell of claim 5 , wherein the medium-chain methyl ketone comprises at least one of 2-heptanone, 2-nonanone, and 2-undecanone.

7 . A recombinant cell for producing methyl ketones comprising:

a recombinant β-ketoacyl-CoA thioesterase gene, wherein the recombinant β-ketoacyl-CoA thioesterase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:8;

a recombinant acyl-ACP thioesterase gene, wherein the recombinant acyl-ACP thioesterase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:2;

a recombinant acyl-CoA synthetase gene, wherein the recombinant acyl-CoA synthetase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:12;

a functional deletion of a native acyl-CoA dehydrogenase gene;

a functional deletion of at least one of a native enoyl-CoA hydratase gene and a native 3-hydroxyacyl-CoA dehydrogenase gene; and

at least one of:

a functional deletion of a native fadR gene or a homolog thereof; and

a recombinant 3-ketoacyl-CoA thiolase gene that encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO: 22 or SEQ ID NO:242,

wherein the recombinant cell exhibits enhanced production of 2-nonanone with respect to a corresponding native cell.

8 . The recombinant cell of claim 1 , wherein the recombinant cell is a bacterium.

9 . The recombinant cell of claim 1 , wherein the recombinant cell is an E coli.

10 . A recombinant cell for producing methyl ketones comprising:

a recombinant β-ketoacyl-CoA thioesterase gene, wherein the recombinant β-ketoacyl-CoA thioesterase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:8;

a recombinant acyl-ACP thioesterase gene, wherein the acyl-ACP thioesterase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO: 2;

a recombinant acyl-CoA synthetase gene, wherein the recombinant acyl-CoA synthetase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:12;

a functional deletion of a native acyl-CoA dehydrogenase gene;

a recombinant acyl-CoA dehydrogenase gene that encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:30;

a functional deletion of a native 3-ketoacyl-CoA thiolase gene; and

at least one of:

a functional deletion of a native fadR gene or a homolog thereof;

a recombinant enoyl-CoA hydratase gene; and

a recombinant 3-hydroxyacyl-CoA dehydrogenase gene,

wherein the recombinant cell exhibits enhanced production of 2-heptanone with respect to a corresponding native cell.

11 . A recombinant cell for producing methyl ketones comprising:

a recombinant β-ketoacyl-CoA thioesterase gene, wherein the recombinant β-ketoacyl-CoA thioesterase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:8;

a recombinant acyl-ACP thioesterase gene, wherein the acyl-ACP thioesterase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO: 4;

a recombinant acyl-CoA synthetase gene, wherein the recombinant acyl-CoA synthetase gene encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:10;

a functional deletion of a native acyl-CoA dehydrogenase gene;

a recombinant acyl-CoA dehydrogenase gene that encodes a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:30;

a functional deletion of a native 3-ketoacyl-CoA thiolase gene; and

at least one of:

a functional deletion of a native fadR gene or a homolog thereof;

a recombinant enoyl-CoA hydratase gene encoding a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:16; and

a recombinant 3-hydroxyacyl-CoA dehydrogenase gene encoding a protein comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO: 16,

wherein the recombinant cell exhibits enhanced production of 2-undecanone with respect to a corresponding native cell.

Assignments (2)
CONFIRMATORY LICENSE Recorded Jan 30, 2025
From: WISCONSIN ALUMNI RESEARCH FOUNDATION
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 070056/0140 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 24, 2024
From: PFLEGER, BRIAN; HERNANDEZ LOZADA, NESTOR; YAN, QIANG; SIMMONS, TREVOR
To: WISCONSIN ALUMNI RESEARCH FOUNDATION
Reel/Frame 069009/0287 →
Continuity (2)
Provisional Application 63027872 · May 20, 2020
Related Publication 20230183759A1 · Jun 15, 2023
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