Oral delivery system for bioactive agents
The present invention relates to a functional feed and an oral delivery system for delivery of bioactive macromolecules. The oral delivery system comprises ethylenediammonium alginate which is a vehicle for delivery of macromolecular drugs. The oral delivery system according to the present invention is particularly suitable for use in combination with functional feeds in fish.
1. A method of therapeutically or prophylactically treating fish, the method comprising:
administering to the fish an oral delivery system, the oral delivery system comprising a fish feed pellet comprising ethylenediammonium alginate particles having a mean particle size of no more than 2000 microns, the fish feed pellet comprising an oil suspension wherein the ethylenediammonium alginate particles are vacuum coated thereon, the ethylenediammonium alginate particles comprising
ethylenediammonium alginate; and
a bioactive agent that is encapsulated or entrapped in the ethylenediammonium alginate;
wherein the fish is a coldwater fish that lives in average water temperatures of about 20° C. or lower.
2. The method of claim 1 , wherein the fish is an omnivorous or carnivorous species.
3. The method of claim 1 , wherein the fish is a gastric fish, a fish with a short intestinal tract, salmonids, basses, breams, codfish, halibut, turbot, flounders, grouper, tuna, tilapia, or pangasius.
4. The method of claim 1 , wherein the bioactive agent is selected from the group consisting of proteins, peptides, vaccines, antibodies, antigens, hormones, enzymes, immune stimulants, drugs, probiotics, prebiotics, polynucleotides, nucleotides, and amino acids.
5. The method of claim 1 , wherein the mean particle size of the ethylenediammonium alginate particles is no more than 300 μm.
6. The method of claim 1 , wherein the particles comprise beads.
7. The method of claim 1 , wherein the mean particle size of the ethylenediammonium alginate particles is in the range of 300 μm to 2000 μm.
8. The method of claim 1 , wherein the mean particle size of the ethylenediammonium alginate particles is no more than 100 μm.
9. The method of claim 1 , wherein the mean particle size of the ethylenediammonium alginate particles is no more than 200 μm.