POLYPEPTIDES FOR USE IN SELF-ASSEMBLING PROTEIN NANOSTRUCTURES
Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.
1 .- 20 . (canceled)
21 . A self-assembling icosahedral protein nanostructure, comprising:
20 trimeric components having 3-fold symmetry, each trimeric component comprising 3 first subunits, wherein each first subunit comprises a polypeptide having a polypeptide sequence that is at least 80% identical to SEQ ID NO: 7 or 29-31; and
12 pentameric components having 5-fold symmetry, each pentameric component comprising 5 second subunits, wherein each second subunit comprises a polypeptide having a polypeptide sequence that is at least 80% identical to SEQ ID NO: 8 or 32-34.
22 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is at least 90% identical to SEQ ID NO: 7 or 29-31; and
wherein the polypeptide sequence of each second subunit is at least 90% identical to SEQ ID NO: 8 or 32-34.
23 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is at least 95% identical to SEQ ID NO: 7 or 29-31; and
wherein the polypeptide sequence of each second subunit is at least 95% identical to SEQ ID NO: 8 or 32-34.
24 . The nanostructure of claim 23 , wherein the trimeric components and the pentameric components form an I53 icosahedral architecture.
25 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 7; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 8.
26 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 7; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 32.
27 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 7; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 33.
28 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 7; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 34.
29 . The nanostructure of claim 28 , wherein the trimeric components and the pentameric components form an I53 icosahedral architecture.
30 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 29; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 8.
31 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 29; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 32.
32 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 29; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 33.
33 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 29; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 34.
34 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 30; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 8.
35 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 30; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 32.
36 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 30; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 33.
37 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 30; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 34.
38 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 31; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 8.
39 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 31; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 32.
40 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 31; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 33.
41 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 31; and
wherein the polypeptide sequence of each second subunit is SEQ ID NO: 34.
42 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is at least 95% identical to SEQ ID NO: 7.
43 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is at least 95% identical to SEQ ID NO: 29.
44 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is at least 95% identical to SEQ ID NO: 30.
45 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is at least 95% identical to SEQ ID NO: 31.
46 . The nanostructure of claim 21 , wherein the polypeptide sequence of each second subunit is at least 95% identical to SEQ ID NO: 8.
47 . The nanostructure of claim 21 , wherein the polypeptide sequence of each second subunit is at least 95% identical to SEQ ID NO: 32.
48 . The nanostructure of claim 21 , wherein the polypeptide sequence of each second subunit is at least 95% identical to SEQ ID NO: 33.
49 . The nanostructure of claim 21 , wherein the polypeptide sequence of each second subunit is at least 95% identical to SEQ ID NO: 34.
50 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 7.
51 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 29.
52 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 30.
53 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is SEQ ID NO: 31.
54 . The nanostructure of claim 21 , wherein the polypeptide sequence of each second subunit is SEQ ID NO: 8.
55 . The nanostructure of claim 21 , wherein the polypeptide sequence of each second subunit is SEQ ID NO: 32.
56 . The nanostructure of claim 21 , wherein the polypeptide sequence of each second subunit is SEQ ID NO: 33.
57 . The nanostructure of claim 21 , wherein the polypeptide sequence of each second subunit is SEQ ID NO: 34.
58 . The nanostructure of claim 21 , wherein the polypeptide sequence of each first subunit is identical to SEQ ID NO: 7 or 29-31 at at least 3 of interface amino acid positions 25, 29, 33, 54, and 57; and
wherein the polypeptide sequence of each second subunit is identical to SEQ ID NO: 8 or 32-34 at at least 3 of interface amino acid positions 24, 28, 36, 124, 125, 127, 128, 129, 131, 132, 133, 135, and 139.
59 . The nanostructure of claim 23 , wherein the polypeptide sequence of each first subunit is identical to SEQ ID NO: 7 or 29-31 at at least 3 of interface amino acid positions 25, 29, 33, 54, and 57; and
wherein the polypeptide sequence of each second subunit is identical to SEQ ID NO: 8 or 32-34 at at least 3 of interface amino acid positions 24, 28, 36, 124, 125, 127, 128, 129, 131, 132, 133, 135, and 139.
60 . The nanostructure of claim 24 , wherein the polypeptide sequence of each first subunit is identical to SEQ ID NO: 7 or 29-31 at at least 3 of interface amino acid positions 25, 29, 33, 54, and 57; and
wherein the polypeptide sequence of each second subunit is identical to SEQ ID NO: 8 or 32-34 at at least 3 of interface amino acid positions 24, 28, 36, 124, 125, 127, 128, 129, 131, 132, 133, 135, and 139.