FLOW CELLS WITH DARK QUENCHER
An example flow cell includes a substrate having a surface. The flow cell also includes a polymeric hydrogel attached to at least a portion of the substrate surface, where the polymeric hydrogel includes a dark quencher. The flow cell further includes at least one primer set attached to the polymeric hydrogel.
1 . A flow cell, comprising:
a substrate having a surface;
a polymeric hydrogel attached to at least a portion of the substrate surface, the polymeric hydrogel including a dark quencher; and
at least one primer set attached to the polymeric hydrogel.
2 . The flow cell as defined in claim 1 , wherein the polymeric hydrogel is applied to the substrate surface as a plurality of pads, wherein each of the plurality of pads is isolated from each other of the plurality of pads by interstitial regions.
3 . The flow cell as defined in claim 1 , wherein:
the substrate includes a plurality of depressions defined in the substrate surface that are isolated from each other by interstitial regions; and
each of the plurality of depressions has the polymeric hydrogel applied therein.
4 . The flow cell as defined in claim 1 , wherein:
the substrate includes a lane defined in the substrate surface; and
the lane has the polymeric hydrogel applied therein.
5 . The flow cell as defined in claim 1 , wherein the polymeric hydrogel includes an acrylamide copolymer.
6 . The flow cell as defined in claim 1 , wherein the dark quencher is selected from the group consisting of dimethylaminoazobenzenesulfonic acid, 4′-(2-nitro-4-toluyldiazo)-2′-methoxy-5′-methyl-azobenzene-4″-(N-ethyl)-N-ethyl-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite, 4′-(4-nitro-phenyldiazo)-2′-methoxy-5′-methoxy-azobenzene-4″-(N-ethyl)-N-ethyl-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite, 5′-Dimethoxytrityloxy-5-[(N-4″-carboxyethyl-4″-(N-ethyl)-4′-(2-Nitro-4-toluyldiazo)-2′-methoxy-5′-methyl-azobenzene)-aminohexyl-3-acrylimido]-2′-deoxyUridine-3′-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite, 5′-Dimethoxytrityloxy-5-[(N-4″-carboxyethyl-4″-(N-ethyl)-4′-(4-Nitro-phenyldiazo)-2′-methoxy-5′-methoxy-azobenzene)-aminohexyl-3-acrylimido]-2′-deoxyUridine-3′-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite, and combinations thereof.
7 . The flow cell as defined in claim 1 , wherein:
the substrate is a multi-layer substrate including:
a base support; and
a resin layer positioned on the base support; and
the polymeric hydrogel is attached to at least a portion of the resin layer.
8 . The flow cell as defined in claim 1 , wherein the polymeric hydrogel has the dark quencher removably attached through a cleavable linking molecule.
9 . The flow cell as defined in claim 8 , wherein the cleavable linking molecule includes a non-covalent binding pair.
10 . The flow cell as defined in claim 9 , wherein the dark quencher is present in an amount ranging from about 0.25 mol % to about 50 mol % relative to a total number of moles in the polymeric hydrogel.
11 . The flow cell as defined in claim 1 , wherein the polymeric hydrogel has the dark quencher incorporated into its backbone chain.
12 . The flow cell as defined in claim 11 , wherein the dark quencher is present in an amount ranging from about 0.25 mol % to about 50 mol % relative to a total number of moles in the polymeric hydrogel.
13 . The flow cell as defined in claim 1 , wherein the dark quencher is covalently attached to the polymeric hydrogel through a linking molecule.
14 . The flow cell as defined in claim 13 , wherein:
the linking molecule is a non-oligonucleotide linker; and
the linking molecule is present in an amount ranging from about 0.25 mol % to about 50 mol % relative to a total number of moles in the polymeric hydrogel.
15 . The flow cell as defined in claim 13 , wherein:
the linking molecule is an oligonucleotide linker;
the polymeric hydrogel includes a plurality of a functional group that is to attach to the oligonucleotide linker; and
the oligonucleotide linker is present in an amount sufficient to occupy from about 0.5% to about 50% of the plurality of the functional group.
16 . The flow cell as defined in claim 1 , wherein the dark quencher is attached to the polymeric hydrogel through a hairpin oligonucleotide or DNA origami.
17 . The flow cell as defined in claim 1 , wherein the dark quencher exhibits absorption at one or more wavelengths ranging from about 400 nm to about 670 nm.
18 .- 34 . (canceled)