NUCLEIC ACID BASED DATA STORAGE
Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Additionally, devices described herein for de novo synthesis of nucleic acids encoding information related to the original source information may be rigid or flexible material. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Also provided herein are methods and systems for efficient transfer of preselected polynucleotides from a storage structure for reading stored information.
1 . A method comprising:
a. converting at least one item of information in a form of at least one digital sequence to at least one nucleic acid sequence;
b. synthesizing a plurality of polynucleotides having predetermined sequences collectively encoding for the at least one nucleic acid sequence; and
c. selectively transferring the plurality of polynucleotides to a receiving unit, wherein selectively transferring comprises application of a force.
2 . The method of claim 1 , wherein selectively transferring comprises transferring the plurality of polynucleotides through one or more channels.
3 . The method of claim 2 , wherein selectively transferring comprises transferring the plurality of polynucleotides in a transfer media.
4 . The method of claim 3 , wherein selectively transferring comprises transferring in the transfer media comprising an aqueous transfer media or a gaseous transfer media.
5 . The method of claim 1 , wherein selectively transferring comprises transferring the plurality of polynucleotides using one or more conducting members.
6 . The method of claim 1 , wherein selectively transferring comprises transferring the plurality of polynucleotides using a pressure release or pressure nozzle.
7 . The method of claim 1 , wherein selectively transferring comprises transferring to the receiving unit comprising one or more compartments.
8 . The method of claim 7 , wherein transferring comprises each of the one or more compartments receiving at least a portion of the plurality of polynucleotides.
9 . The method of claim 1 , further comprising amplifying the plurality of polynucleotides.
10 . The method of claim 1 , further comprising controlling, via a computer system, synthesis, amplification, or selective transfer of the plurality of polynucleotides.
11 . The method of claim 1 , further comprising storing the plurality of polynucleotides.
12 . The method of claim 1 , further comprising sequencing the plurality of polynucleotides.
13 . The method of claim 12 , further comprising converting sequences of the plurality of polynucleotides to binary code.
14 . The method of claim 1 , wherein the force comprises laminar pressure, capillary pressure, slip flow pressure, magnetic force, electrostatic force, peristaltic force, sound waves, vibrational force, centripetal force, centrifugal force, or any combination thereof.
15 . The method of claim 1 , wherein synthesizing comprises extending each of the plurality of polynucleotides from a structure comprising a surface.
16 . The method of claim 15 , further comprising cleaving the plurality of polynucleotides from the surface.
17 . The method of claim 16 , wherein cleaving the plurality of polynucleotides comprises application of a restriction enzyme, electromagnetic radiation, or a gaseous reagent.
18 . The method of claim 15 , wherein synthesizing comprises synthesizing the plurality of polynucleotides on the surface comprising a plurality of loci.
19 . The method of claim 18 , wherein synthesizing comprises each of the plurality of loci supporting synthesis of a population of polynucleotides having a different sequence than a population of polynucleotides grown on another locus.
20 . The method of claim 18 , wherein synthesizing comprises each of the plurality of loci being individually accessible.