Carotenoid compound coming from plant and containing natural astaxanthin, preparation method therefor, and composition
The present invention provides a high-content carotenoid compound from Adonis amurensis . The content of the total carotenoid of the carotenoid compound is higher than 95%. The content of all-trans (3S, 3′S)-carotenoid is higher than 80%. The carotenoid crystals have a high purity, and can be used in multiple forms in the fields of a dietary supplement of a human being, a food additive, a feed additive and a cosmetic product. In addition, the present invention also provides a method for manufacturing the compound.
1 . A method of preparing a high-content carotenoid mixture by using adonis amurensis extract as raw material, characterized in that, comprising the following steps:
(a) mixing Adonis amurensis extract with alcohols solvent and fully dissolving, to obtain a mixed solution;
(b) heating the mixed solution of the step (a) to 25-75° C., and dropwise adding an alkali solution to the reaction, and the reaction time is 0.5˜ 6.0 hr, wherein a concentration of the alkali solution of the reaction is 0.01-2.50 mol/L after adding dropwise the alkali solution;
(c) adding a mixture of alcohols solvent and water to obtain a reaction solution, after completion of the step (b), wherein a volume ratio of the alcohols solvent to water in the step (c) is 1:5-10;
(d) filtering the reaction solution of the step (c) to obtain a filter cake, and then washing the filter cake with a mixture of alcohols solvent and water, wherein a volume ratio of the alcohols solvent to water in the step (d) is 2-3:1; and
(e) drying the filter cake of the step (d) in vacuum to obtain a carotenoid mixture;
wherein, in the high-content carotenoid mixture, the content of the total carotenoid is higher than 95 wt %, the proportion of the all-trans (3S, 3'S) astaxanthin in the carotenoid is higher than 80 wt %, the all-trans (3S, 3'S)-astaxanthin has the molecular structure as shown in formula (I):
2 . The method according to claim 1 , characterized in that, in the step (a), the ratio of the volume of the alcohols solvent to the weight of Adonis amurensis extract is 25˜40 times.
3 . The method according to claim 1 , characterized in that, in the step (b), the concentration of the alkali solution of the reaction is 1.00-2.00 mol/L after adding dropwise the alkali solution.
4 . The method according to claim 2 , characterized in that, wherein the alcohols solvent in the step (a) is ethanol, propylene glycol or glycerol; and the alkali solution in step (b) is sodium hydroxide solution, potassium hydroxide solution or sodium methoxide solution.