Atraumatically formed tissue compositions, devices and methods of preparation and treatment
A process and system provides for atraumatic preparation of morselized Tissue Particles (TP)s, such as Full Thickness Skin Graft Particles (FTSGPs), cartilage particles and other organ tissue particles, in a liquid medium. The resultant tissue product may be a suspension of Tissue Particles in an aqueous solution and containing highly viable cells and may be rapidly prepared at bedside or in the operating room and conveniently delivered to a patient through a syringe or similar applicator. The morselized Tissues Particles may be used for surgical applications including wound healing, cosmetic surgery, and orthopedic cartilage repairs.
1 . Tissue particles, harvested from a full thickness skin graft and randomly morselized repeatedly to a random size and shape in a continuous circulating stream of a solution and full thickness skin particles, while maintaining a cell viability of at least 60%, said particles having an average size as measured across a largest dimension selected from the group consisting of: about 0.35 mm to 1.25 mm, about 0.5 mm to about 1.00 mm, about 0.5 mm to about 0.75 mm, about 0.25 mm to about 0.75 mm, and about 0.50 mm.
2 . The morselized tissue particles of claim 1 , wherein the viability is at least 85%.
3 . The morselized tissue particles of claim 1 , wherein the viability is selected from the group consisting of: at least 90%, at least 92%, at least 94%, at least 96%, at least 97%, at least 98%, at least 99%, and 87-98%.
4 . The morselized tissue particles of claim 1 , wherein an average size as measured across a largest dimension is about 0.25 mm to 0.75 mm, and wherein the viability is at least 90%.
5 . The morselized tissue particles of claim 1 , wherein an average size as measured across a largest dimension is about 0.50 mm.
6 . A composition comprising the morselized tissue particles of claim 1 , wherein the composition is in a form selected from the group consisting of a liquid, suspension, dispersion, cream, fluid, emulsion, paste, spray, and injectable.
7 . The composition of claim 6 , further comprising at least one selected from the group consisting of a liquid medium, fibrin glue, autologous platelet rich plasma, growth factors, binders, saline, buffer solution, and cell nurturing/preservation solution.
8 . The composition of claim 7 , wherein the liquid medium is a hydrophilic medium, an oleophilic medium, or an emulsion of hydrophilic and oleophilic mediums.
9 . The composition of claim 6 , wherein the composition is in the form of a suspension, further wherein the suspension is heterogeneous.
10 . The composition of claim 6 , wherein the composition is in the form of a suspension, further wherein the suspension is homogenous.
11 . A surgical procedure comprising:
harvesting the tissue particles of claim 1 ;
processing the harvested tissue for less than 1 hour to prepare morselized tissue particles, wherein the morselized tissue particles have an average size as measured across a largest dimension of about 0.25 mm to 0.75 mm and a viability of at least 85%; and
implanting the morselized tissue particles in a joint of the patient.
12 . Morselized tissue particles, having an average size as measured across a largest dimension of about 0.20 mm to 1.50 mm, and a viability of at least 60%,
wherein the morselized tissue particles are formed using a device for processing organ tissue comprising:
a pair of cutting devices supported in juxtaposition in a container, wherein at least one of the cutting devices is moveable with respect the other cutting device to slice tissue between thereof;
wherein at least one of the cutting devices is fixed with respect the moveable cutting device; and
wherein an agitation device causes a vortex propulsion movement of fluid and tissue through the space between juxtaposed cutting devices.
13 . The morselized tissue particles of claim 12 , wherein the viability is at least 70%.
14 . The morselized tissue particles of claim 12 , wherein the viability is at least 85%.
15 . The morselized tissue particles of claim 12 , wherein the morselized tissue particles are morselized cartilage tissue particles.
16 . The morselized cartilage tissue particles of claim 15 , wherein the viability is at least 85%.
17 . The morselized cartilage tissue particles of claim 15 , wherein the viability is at least 92%.
18 . The morselized cartilage tissue particles of claim 15 , wherein the viability is at least 96%.
19 . The morselized cartilage tissue particles of claim 15 , wherein the viability is 87-98%.
20 . The morselized cartilage tissue particles of claim 15 , having an average size as measured across a largest dimension of about 0.50 mm and a viability of at least 90%.
21 . A composition comprising the morselized cartilage tissue particles of claim 15 , wherein the composition is in a form selected from the group consisting of a liquid, suspension, dispersion, cream, fluid, emulsion, paste, spray, and injectable.
22 . The composition of claim 21 , wherein the morselized cartilage tissue particles have an average size as measured across a largest dimension of about 0.50 mm.
23 . The composition of claim 21 , further comprising at least one selected from the group consisting of a liquid medium, fibrin glue, autologous platelet rich plasma, growth factors, binders, saline, buffer solution, and cell nurturing/preservation solution.
24 . A method of repairing cartilage or treating a cartilage defect in a patient in need thereof, comprising administering the composition of claim 21 to the patient.
25 . The method of claim 24 , wherein the morselized cartilage tissue particles have an average size as measured across a largest dimension of about 0.50 mm.
26 . The method of claim 25 , wherein the composition is administered to the patient via syringe or spatula.
27 . The method of claim 24 , wherein the composition is administered to a joint of the patient.
28 . The method of claim 24 , wherein the morselized cartilage tissue particles is morselized articular cartilage tissue particles.
29 . The morselized cartilage tissue particles of claim 15 , wherein the source of the cartilage tissue is non-articular cartilage, articular cartilage, or a combination thereof.
30 . A cartilage graft, comprising the morselized cartilage tissue particles of claim 15 .
31 . A surgical procedure comprising:
harvesting the tissue particles of claim 12 ;
processing the harvested tissue for less than 1 hour to prepare morselized tissue particles, wherein the morselized tissue particles have an average size as measured across a largest dimension of about 0.25 mm to 0.75 mm and a viability of at least 85%; and
implanting the morselized tissue particles in a joint of the patient.
32 . The surgical procedure of claim 31 , wherein:
the at least one tissue portion is at least one cartilage tissue portion,
the harvested tissue is harvested cartilage, and
the morselized tissue particles are morselized cartilage tissue particles.
33 . The surgical procedure of claim 31 , wherein the harvested cartilage is harvested from the patient's non-articular cartilage or articular cartilage.
34 . Viable tissue particles for treating a patient in need thereof, said tissue particles being harvested from a full thickness skin graft and randomly repeatedly morselized to a size and shape in a continuous circulating stream of a solution and full thickness skin particles, said continuous stream generated by an agitation device which produces a vortex propulsion between juxtaposed cutting devices, said particles having an average size as measured across a largest dimension selected from the group consisting of: about 0.35 mm to 1.25 mm, about 0.5 mm to about 1.00 mm, about 0.5 mm to about 0.75 mm, about 0.25 mm to about 0.75 mm, and about 0.50 mm.