PREBIOTIC FORMULATIONS
Provided herein are compositions comprising a biocompatible microsphere, a biofilm-generating probiotic bacterium, a prebiotic, and/or a prebiofilmic. Methods for preparing and formulating the compositions and methods for treating or preventing a disease using the compositions are also provided.
1 - 71 . (canceled)
72 . A method of treating depression or anxiety in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a composition comprising: a strain of Lactobacillus reuteri (“ L. reuteri ”) that expresses glucosyltransferase (GTF) protein; a biocompatible microsphere; and a water-soluble carbohydrate, thereby treating depression or anxiety.
73 . The method of claim 72 , wherein the L. reuteri is adhered to the biocompatible microsphere.
74 . The method of claim 72 , wherein the strain of L. reuteri is selected from the group consisting of ATCC 23272 , L. reuteri strain 100-23, ATCC PTA 6475, ATCC 55730, DSM 17938 and combination(s) thereof.
75 . The method of claim 72 , wherein the water-soluble carbohydrate is selected from the group consisting of maltose, sucrose, and combinations thereof.
76 . The method of claim 72 , wherein the biocompatible microsphere has a diameter of about 1 microns to about 100 microns.
77 . The method of claim 72 , wherein the biocompatible microsphere comprises dextran cross-linked with epichlorohydrin.
78 . The method of claim 72 , wherein the subject also has a gastrointestinal disorder.
79 . The method of claim 72 , wherein administration comprises an oral administration.
80 . The method of claim 72 , wherein the composition is administered to provide from about 1×10 7 to about 1×10 11 CFU/ml of L. reuteri.
81 . The method of claim 72 , wherein the composition is administered in a single dose.
82 . The method of claim 72 , wherein the composition is administered in multiple doses.