Cancer test device, cancer test method, and staining agent for use in cancer test
A cancer test device ( 1 ) is provided with: an application unit ( 40 ) for applying a staining agent ( 45 ), which can selectively stain a product of a cancer-relating gene in a living cell a chromatic color, onto a group of living cells; an imaging unit ( 10 ) for imaging the group of living cells having the staining agent ( 45 ) applied thereto; and a determination unit ( 52 ) for determining the level of malignancy of cancerization of the group of living cells on the basis of the state of the stained expression pattern of the cancer-relating gene in the group of living cells in an image obtained by the aforementioned imaging.
1. A cancer test method comprising:
an application step of applying a stain that selectively stains a cancer-related gene product of living cells in a chromatic color to a living cell group, wherein, the cancer-related gene product is STAT3-family cancer-related gene product that transmits a signal that promotes growth of the living cells and the stain is a curcumin-based compound;
an imaging step of imaging the living cell group to which the stain has been applied; and
an evaluation step of evaluating a grade of cancerization of the living cell group based on a staining state of the living cell group in an image produced by the imaging.
2. The cancer test method according to claim 1 ,
wherein an application unit applies the stain containing a curcumin-based compound to the living cell group and then applies the stain that stains a ras-family cancer-related gene product that transmits a signal that promotes growth of the living cells, wherein the stain is phloxine, erythrosine, merbromin, fast green FCF, or meclocycline sulfosalicylate to the living cell group.
3. The cancer test method according to claim 1 ,
wherein in the evaluation step, the evaluation is performed based on an area of a stained region of the living cell group.
4. The cancer test method according to claim 1 ,
wherein in the evaluation step, the evaluation is performed based on number of stained cells in a stained region of the living cell group.
5. The cancer test method according to claim 1 ,
wherein in the evaluation step, the evaluation is performed based on number and average diameter of stained cell groups in a fixed area containing a stained region of the living cell group.