Granted Patent
B2
US 12,703,877 · App. 18/000,797 · Granted Aug 11, 2026
Methods and systems to secrete lignin-modifying enzymes and uses thereof
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Abstract
Embodiments of the invention are generally directed to lignin-modifying enzymes and systems and methods of their manufacture. In many embodiments, yeast strains, including S. cerevisiae , are used to produce and secrete lignin-modifying enzymes Further embodiments are directed to methods to screening peroxidase-producing yeast strains, including S. cerevisiae . Additional embodiments are directed to an expression vector or cassette encoding for a protein of interest and one or more proteins to allow for surface to display of the protein of interest.
Claims (7)
1 . A transgenic yeast organism comprising:
a knockout of a first native gene and a knockout of a second native gene, wherein the first native gene is cyt2 encoding cytochrome c heme lyase and the second native gene is pmt2 encoding protein O-mannosyltransferase 2; and
an expression cassette comprising a nucleotide sequence encoding for a lignin-modifying enzyme.
2 . The transgenic yeast organism of claim 1 , wherein the lignin-modifying enzyme is a heme peroxidase.
3 . The transgenic yeast organism of claim 1 , wherein the nucleotide sequence encoding the lignin-modifying enzyme is one of SEQ ID NOs: 1-78.
4 . The transgenic yeast organism of claim 1 , wherein the expression cassette further comprises a nucleotide sequence encoding a scaffold protein and a surface display protein, wherein the surface display protein is linked to the lignin-modifying enzyme, such that a hybrid peptide is produced with the lignin-modifying enzyme and the surface display protein.
5 . The transgenic yeast organism of claim 1 , wherein the expression cassette further comprises an inducible promoter operatively linked to the nucleotide sequence encoding the lignin-modifying enzyme.
Assignments (2)
CONFIRMATORY LICENSE
Recorded Feb 15, 2024
From: STANFORD UNIVERSITY
To: U.S. DEPARTMENT OF ENERGY
Reel/Frame 066601/0122 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 14, 2023
From: KHLYSTOV, NIKITA A.
To: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Reel/Frame 064909/0231 →
Continuity (2)
Provisional Application
63035172
· Jun 5, 2020
Related Publication
20230313257A1
· Oct 5, 2023
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