High-stability surface-enhanced raman scattering nanotag and making method therefor
A nanotag includes a gold core, the gold core is sequentially coated with a silver shell and a gold shell, and a Raman dye is arranged between the silver shell and the gold shell; further it discloses a making method for a high-stability SERS nanotag that includes the following steps: a. adding tetrachloroauric acid into ultrapure water, after boiling, quickly adding trisodium citrate, continuously boiling for a few minutes, stopping heating, and cooling; b. adding hexadecyl trimethyl ammonium chloride (CTAC) into product obtained in the step a, stirring, and then adding ascorbic acid and AgNO 3 , and centrifugating and resuspending in the CTAC; c. adding CTAC into product obtained in the step b, stirring, adding a Raman dye, ascorbic acid and sodium hydroxide, injecting a mixture of tetrachloroauric acid and potassium iodide, and centrifuge the mixture and resuspending in the CTAC.
1. A method for preparing a high-stability SERS nanotag, comprising the following steps:
(a) adding tetratetrachloroauric acid into ultrapure water, quickly adding a reducing agent with a mass to volume ratio of 1% to 2% after boiling, continuously carrying out boiling, stopping heating, and carrying out cooling to 24° C. to 37° C.;
(b) adding CTAC with a concentration of 0.01 mM to 1 mM into a substance obtained in the step (a), carrying out stirring, adding ascorbic acid, injecting AgNO 3 , and carrying out centrifugation after reaction and resuspending in CTAC with a concentration of 1 mM to 10 mM; and
(c) adding CTAC with a concentration of 20 mM to 100 mM into a substance obtained in the step (b), carrying out stirring, sequentially adding
a Raman dye, ascorbic acid and sodium hydroxide, injecting a mixture of tetratetrachloroauric acid and potassium iodide, and carrying out centrifugation after reaction and resuspending in CTAC with a concentration of 1 mM to 10 mM;
wherein the high-stability SERS nanotag comprises a gold core ( 1 ), wherein the gold core ( 1 ) is sequentially coated with a silver shell ( 2 ) and a gold shell ( 3 ), and a Raman dye ( 4 ) is arranged between the silver shell ( 2 ) and the gold shell ( 3 ).
2. The method according to claim 1 , wherein in the step (a), the reducing agent is trisodium citrate, sodium borohydride, white phosphorus or ascorbic acid.
3. The method according to claim 1 , wherein in the step (c), the CTAC with a concentration of 20 mM to 100 mM has a volume of 1 mL to 100 mL, and the substance obtained in the step (b) has a volume of 1 mL to 100 mL.
4. The method according to claim 1 , wherein in the step (c), the Raman dye has a volume of 0.1 mL to 1 mL and a concentration of 0.1 mM to 10 mM, the ascorbic acid has a volume of 10 mL to 100 mL and a concentration of 50 mM to 200 mM, and the sodium hydroxide has a volume of 10 mL to 100 mL and a concentration of 100 mM to 400 mM.
5. The method according to claim 1 , wherein in the step (c), an injection speed is 50 μl/min to 1000 μl/min, the mixture of tetrachloroauric acid and potassium iodide has a volume of 50 mL to 500 mL, the tetrachloroauric acid has a concentration of 0.1 mM to 0.5 mM, and the potassium iodide has a concentration of 0.1 mM to 2 mM.
6. The method according to claim 1 , wherein the gold core ( 1 ) has a particle size of 5 nm to 100 nm.
7. The method according to claim 1 , wherein the silver shell ( 2 ) has a thickness of 2 nm to 50 nm.
8. The method according to claim 1 , wherein the gold shell ( 3 ) has a thickness of 1 nm to 10 nm.
9. The method according to claim 1 , wherein the Raman dye ( 4 ) is one or more of 4-Mercaptobenzoic acid, 5,5′-Dithiobis (2-nitrobenzoic acid), nile blue, methylene blue, crystal violet, malachite green and rhodamine 6G.