INHT26 TRANSGENIC SOYBEAN
Transgenic INHT26 soybean plants comprising modifications of the DAS44406-6 soybean locus which provide for facile excision of the modified DAS44406-6 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.
1 . A transgenic soybean plant cell comprising an INHT26 transgenic locus comprising an originator guide RNA recognition site (OgRRS) in a first DNA junction polynucleotide of a DAS44406-6 transgenic locus and a cognate guide RNA recognition site (CgRRS) in a second DNA junction polynucleotide of the DAS44406-6 transgenic locus.
2 . A transgenic soybean plant cell comprising an INHT26 transgenic locus comprising an insertion and/or substitution of DNA in a DNA junction polynucleotide of a DAS44406-6 transgenic locus with DNA comprising a cognate guide RNA recognition site (CgRRS).
3 . The transgenic soybean plant cell of claim 1 or 2 , wherein said CgRRS comprises the DNA molecule set forth in SEQ ID NO: 17 or 16; and/or wherein said DAS44406-6 transgenic locus is set forth in SEQ ID NO:1, is present in seed deposited at the ATCC under accession No. PTA-11336, is present in progeny thereof, is present in allelic variants thereof, or is present in other variants thereof.
4 . The transgenic soybean plant cell of claim 1 or 2 , wherein said INHT26 transgenic locus comprises the DNA molecule set forth in SEQ ID NO: 14.
5 . A transgenic soybean plant part comprising the soybean plant cell of any one of claim 1 or 2 , wherein said soybean plant part is optionally a seed.
6 . A transgenic soybean plant comprising the soybean plant cell of claim 1 or 2 .
7 . A method for obtaining a bulked population of inbred seed comprising selfing the transgenic soybean plant of claim 5 and harvesting seed comprising the INHT26 transgenic locus from the selfed soybean plant.
8 . A method of obtaining hybrid soybean seed comprising crossing the transgenic soybean plant of claim 5 to a second soybean plant which is genetically distinct from the first soybean plant and harvesting seed comprising the INHT26 transgenic locus from the cross.
9 . A DNA molecule comprising SEQ ID NO: 17, 14, or 16.
10 . A processed transgenic soybean plant product comprising the DNA molecule of claim 9 .
11 . A biological sample containing the DNA molecule of claim 9 .
12 . A nucleic acid molecule adapted for detection of genomic DNA comprising the DNA molecule of claim 9 , wherein said nucleic acid molecule optionally comprises a detectable label.
13 . A method of detecting a soybean plant cell comprising the INHT26 transgenic locus of claim 1 or 2 , comprising the step of detecting DNA molecule comprising SEQ ID NO: 17, 14, or 16.
14 . A method of excising the INHT26 transgenic locus from the genome of the soybean plant cell of claim 1 or 2 , comprising the steps of:
(a) contacting the genome of the soybean plant cell with: (i) an RNA dependent DNA endonuclease (RdDe); and (ii) a guide RNA (gRNA) capable of hybridizing to the guide RNA hybridization site of the OgRRS and the CgRRS; wherein the RdDe recognizes a OgRRS/gRNA and a CgRRS/gRNA hybridization complex; and,
(b) selecting a transgenic plant cell, transgenic plant part, or transgenic plant wherein the INHT26 transgenic locus flanked by the OgRRS and the CgRRS has been excised.