Electrophoresis device and biological substance recovery method
An electrophoresis device is used to separate a biological substance using a separation medium. A recovery hole configured to recover the separated target biological substance is formed in an electrophoresis device, the recovery hole is configured to be capable of accommodating a solvent that can suspend the target biological substance, a buffer tank configured to accommodate a buffer solution is provided below the recovery hole, and a membrane is disposed between the recovery hole and the buffer tank.
1 . An electrophoresis device configured to separate a biological substance with a separation medium, the electrophoresis device comprising:
a recovery hole configured to recover a separated target biological substance, the recovery hole being configured to be capable of accommodating a solvent capable of suspending the target biological substance;
a buffer tank configured to accommodate a buffer solution and provided below the recovery hole; and
a membrane disposed between the recovery hole and the buffer tank,
wherein the target biological substance is one type of ion.
2 . A biological substance recovery method using the electrophoresis device according to claim 1 , the biological substance recovery method comprising, before the target biological substance reaches the recovery hole:
removing the buffer solution in the recovery hole;
cleaning an inside of the recovery hole using a cleaning liquid; and
injecting the solvent into the recovery hole,
the biological substance recovery method further comprising recovering the target biological substance after the target biological substance reaches the recovery hole.
3 . A biological substance recovery method using the electrophoresis device according to claim 1 , the biological substance recovery method comprising:
removing a supernatant of the solvent in the recovery hole after the target biological substance reaches the recovery hole; and
recovering the target biological substance in the recovery hole after removing the supernatant.
4 . The biological substance recovery method according to claim 3 , wherein the removing the supernatant of the solvent is performed while continuously applying a voltage.
5 . The electrophoresis device according to claim 1 , wherein the buffer tank has an opening that is open upward and provided in a portion other than a portion where the membrane is disposed.
6 . The electrophoresis device according to claim 1 , further comprising a positive electrode and a negative electrode configured to apply a voltage, wherein the positive electrode is disposed at a lower position than the membrane.
7 . The electrophoresis device according to claim 6 , wherein the positive electrode is installed in a region that is not a region immediately below the membrane.
8 . The electrophoresis device according to claim 1 , further comprising an insulation that covers the separation medium and the recovery hole.
9 . The electrophoresis device according to claim 1 , further comprising an injection hole through which a sample containing the target biological substance is injected.
10 . The electrophoresis device according to claim 1 , wherein a permeability of the membrane with respect to the target biological substance is lower than a permeability of the membrane with respect to at least one other type of ion.
11 . The electrophoresis device according to claim 10 , wherein the membrane does not allow permeation of the target biological substance but allows permeation of the at least one other type ofion.