METHOD OF PRODUCING THREE-DIMENSIONAL TISSUE BODY AND THREE-DIMENSIONAL TISSUE BODY

1 . A method for producing a three-dimensional tissue body, comprising:

a step of obtaining a mixture by mixing a cationic substance and a fragmented extracellular matrix component with cells; and

a step of culturing the cells after the step of obtaining the mixture.

2 . The method for producing a three-dimensional tissue body according to claim 1 ,

wherein the cells comprise at least interstitial cells and endothelial cells.

3 . The method for producing a three-dimensional tissue body according to claim 1 ,

wherein the fragmented extracellular matrix component comprises fragmented collagen.

4 . The method for producing a three-dimensional tissue body according to claim 3 ,

wherein the fragmented collagen is defibrated collagen.

5 . The method for producing a three-dimensional tissue body according to claim 1 ,

wherein, when obtaining the mixture, a polyelectrolyte is furthermore mixed with the cells in addition to the cationic substance and the fragmented extracellular matrix to incorporate the polyelectrolyte into the mixture.

6 . The method for producing a three-dimensional tissue body according to claim 5 ,

wherein the polyelectrolyte comprises at least one selected from the group consisting of glycosaminoglycan, dextran sulfate, rhamnan sulfate, fucoidan, carrageenan, polystyrene sulfonic acid, polyacrylamide-2-methylpropanesulfonic acid, and polyacrylic acid.

7 . The method for producing a three-dimensional tissue body according to claim 5 ,

wherein a concentration of the polyelectrolyte in the mixture is higher than 0 mg/mL and lower than or equal to 1.5 mg/mL.

8 . The method for producing a three-dimensional tissue body according to claim 1 ,

wherein, when obtaining the mixture, an extracellular matrix component is furthermore mixed with the cells simultaneously or separately with respect to the cationic substance and/or the fragmented extracellular matrix.

9 . The method for producing a three-dimensional tissue body according to claim 8 ,

wherein the extracellular matrix component comprises at least one selected from the group consisting of collagen, laminin, fibronectin, vitronectin, elastin, tenascin, entactin, fibrillin, and proteoglycan.

10 . The method for producing a three-dimensional tissue body according to claim 8 ,

wherein a mass ratio of the extracellular matrix component to the fragmented extracellular matrix component is 2:1 to 1:50.

11 . The method for producing a three-dimensional tissue body according to claim 8 ,

wherein a total content of the extracellular matrix component and the fragmented extracellular matrix component in the mixture is 0.005 mg/mL to 1.5 mg/mL.

12 . A three-dimensional tissue body comprising:

cells;

a fragmented extracellular matrix component; and

a polyelectrolyte.

13 . The three-dimensional tissue body according to claim 12 ,

wherein the polyelectrolyte comprises at least one selected from the group consisting of glycosaminoglycan, dextran sulfate, rhamnan sulfate, fucoidan, carrageenan, polystyrene sulfonic acid, polyacrylamide-2-methylpropanesulfonic acid, and polyacrylic acid.

14 . The three-dimensional tissue body according to claim 12 ,

wherein a thickness retention rate represented by an equation below is 80% or higher,

thickness retention rate= X 1 /X 0 ×100  Equation:

[in the equation, X 1 represents a thickness of the three-dimensional tissue body after 4 days of culture, and X 0 represents a thickness of the three-dimensional tissue body at the start of culture].