Compositions comprising IL-15-based molecules and immune checkpoint inhibitor antibodies
The invention features combination therapies using an IL-15-based superagonist complex and an antibody to effectively treat subjects with cancer and infectious diseases.
1. A composition comprising: synergistically effective amounts of an immune checkpoint inhibitor antibody and an IL-15N72D:IL-15RαSu/Fc complex (ALT-803), wherein the immune checkpoint inhibitor antibody is a CTLA-4, PD-1, or PD-L1 antibody, wherein said ALT-803 comprises a dimeric IL-15RaSu/Fc and two IL-15N72D molecules, wherein the composition is formulated for administration to a human subject suffering from a neoplasia comprising the immune checkpoint molecule targeted by the antibody, and wherein the amounts are synergistically effective for treatment of said neoplasia.
2. The composition of claim 1 , wherein the IL-15N72D molecule comprises the amino acid sequence of SEQ ID NO: 3.
3. The composition of claim 1 , wherein the IL-15RαSu/Fc comprises the amino acid sequence of SEQ ID NO: 6.
4. The composition of claim 1 , wherein the neoplasia is selected from the group consisting of a glioblastoma, prostate cancer, hematological cancer, B-cell neoplasms, multiple myeloma, B-cell lymphoma, Hodgkin's lymphoma, chronic lymphocytic leukemia, acute myeloid leukemia, cutaneous T-cell lymphoma, T-cell lymphoma, a solid tumor, urothelial/bladder carcinoma, melanoma, lung cancer, renal cell carcinoma, breast cancer, gastric and esophageal cancer, head and neck cancer, colorectal cancer, ovarian cancer, non-small cell lung carcinoma, B cell non-Hodgkin lymphoma, and squamous cell head and neck carcinoma.
5. The composition of claim 1 , wherein the synergistically effective amount of said ALT-803 is between 0.1 μg/kg and 100 mg/kg.
6. The composition of claim 5 , wherein the synergistically effective amount of said ALT-803 is between 0.1 μg/kg and 1 mg/kg.
7. The composition of claim 1 , wherein the ALT-803 is formulated for systemic administration, intravenous administration, subcutaneous administration, intramuscular administration, intravesical administration or by instillation administration to the subject.
8. The composition of claim 1 , wherein the ALT-803 is formulated for subcutaneous administration to the subject.
9. The composition of claim 1 , wherein the antibody is formulated for intravenous administration to the subject.
10. The composition of claim 1 , wherein the antibody is selected from the group consisting of ipilimumab, nivolumab, pembrolizumab, and durvalumab.