Granted Patent
B2
US 12,509,494 · App. 18/047,754 · Granted Dec 30, 2025
Multi-chain chimeric polypeptides and uses thereof
Assignee:
ImmunityBio, Inc.
C07K14/5434C07K14/54C07K14/5418C07K14/71C07K14/7155C07K14/745C07K16/283C07K2317/622C07K2319/02C07K2319/03C07K2319/30
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Abstract
Provided herein are multi-chain chimeric polypeptides that include: (a) a first chimeric polypeptide including a first target-binding domain, a soluble tissue factor domain, and a first domain of a pair of affinity domains; and (b) a second chimeric polypeptide including a second domain of a pair of affinity domains and a second target-binding domain, where the first chimeric polypeptide and the second chimeric polypeptide associate through the binding of the first domain and the second domain of the pair of affinity domains. Also provided here are methods of using these multi-chain chimeric polypeptides and nucleic acids encoding these multi-chain chimeric polypeptides.
Claims (31)
1 . A method of stimulating an immune cell, wherein the immune cell comprises a CD8+ T cell or a natural killer (NK) cell, the method comprising contacting the immune cell with an effective amount of a multi-chain chimeric polypeptide, wherein the multi-chain chimeric polypeptide comprises:
(a) a first chimeric polypeptide comprising:
(i) a first target-binding domain;
(ii) a soluble tissue factor domain; and
(iii) a first domain of a pair of affinity domains, wherein the first chimeric polypeptide comprises the sequence of SEQ ID NO: 133; and
(b) a second chimeric polypeptide comprising:
(i) a second domain of a pair of affinity domains; and
(ii) a second target-binding domain, wherein the second chimeric polypeptide comprises the sequence of SEQ ID NO: 177;
wherein
the first chimeric polypeptide and the second chimeric polypeptide associate through the binding of the first domain and the second domain of the pair of affinity domains; and
the first target-binding domain and the second target-binding domain each comprise a soluble TGF-β receptor II (TGF-βRII).
2 . The method of claim 1 , wherein the immune cell is contacted in vitro.
3 . The method of claim 1 , wherein the immune cell is contacted in vivo.
4 . The method of claim 1 , wherein the immune cell has previously been genetically modified to express a chimeric antigen receptor or a recombinant T-cell receptor.
5 . The method of claim 1 , wherein the first chimeric polypeptide further comprises one or more additional target-binding domain(s).
6 . The method of claim 1 , wherein the second chimeric polypeptide further comprises one or more additional target-binding domain(s).
7 . The method of claim 1 , wherein:
the first chimeric polypeptide comprises a sequence of SEQ ID NO: 135; and
the second chimeric polypeptide comprises a sequence of SEQ ID NO: 92.
8 . A method of treating cancer in a subject, wherein the cancer is pancreatic cancer, lymphoma, or melanoma, the method comprising administering to the subject a therapeutically effective amount of a multi-chain chimeric polypeptide, the multi-chain chimeric polypeptide comprises:
(a) a first chimeric polypeptide comprising:
(i) a first target-binding domain;
(ii) a soluble tissue factor domain; and
(iii) a first domain of a pair of affinity domains, wherein the first chimeric polypeptide comprises the sequence of SEQ ID NO: 133; and
(b) a second chimeric polypeptide comprising:
(i) a second domain of a pair of affinity domains; and
(ii) a second target-binding domain, wherein the second chimeric polypeptide comprises the sequence of SEQ ID NO: 177; and
wherein
the first chimeric polypeptide and the second chimeric polypeptide associate through the binding of the first domain and the second domain of the pair of affinity domains; and
the first target-binding domain and the second target-binding domain each comprise a soluble TGF-β receptor II (TGF-βRII).
9 . The method of claim 8 , wherein the subject has been identified or diagnosed as having pancreatic cancer, lymphoma, or melanoma.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 17, 2024
From: HCW BIOLOGICS INC.
To: IMMUNITYBIO, INC.
Reel/Frame 068014/0474 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 13, 2023
From: WONG, HING
To: HCW BIOLOGICS, INC.
Reel/Frame 064240/0637 →
Continuity (12)
Provisional Application
62881088
· Jul 31, 2019
Provisional Application
62817230
· Mar 12, 2019
Provisional Application
62817241
· Mar 12, 2019
Provisional Application
62816683
· Mar 11, 2019
Provisional Application
62749506
· Oct 23, 2018
Provisional Application
62749007
· Oct 22, 2018
Provisional Application
62746832
· Oct 17, 2018
Provisional Application
62724969
· Aug 30, 2018
Provisional Application
62725043
· Aug 30, 2018
Provisional Application
62725010
· Aug 30, 2018
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