Genetically encoded biosensors for detection of polyketides
The present disclosure relates to high-throughput detection of polyketides using genetically encoded biosensors.
1. A biosensor system comprising:
a nucleic acid encoding a genetically modified MphR gene sequence, wherein the nucleic acid comprises at least one genetic mutation when compared to the wild-type MphR gene sequence; and
a reporter gene whose transcription is under the control of a promoter region which is regulated by a MphR transcription factor;
wherein the MphR genetic mutation encodes an amino acid change selected from T17R, T27G, T49I, 065M, T27A, M59E, M59S, R22H, K35N, T49I, L89V, D98N, E109D, R122T, K132N, A151T, H184Q, or a combination thereof, in the ligand binding domain.
2. The biosensor system of claim 1 , wherein the nucleic acid encoding the genetically modified MphR gene sequence and the reporter gene are located on one recombinant DNA vector.
3. The biosensor system of claim 1 , wherein the reporter gene is a gene coding for chloramphenicol acetyltransferase, beta-galactosidase, luciferase or green fluorescent protein (GFP).
4. The biosensor system of claim 3 , wherein the reporter gene is a gene coding for green fluorescent protein (GFP).
5. The biosensor system of claim 1 , wherein the mutation confers improved sensitivity for detecting erythromycin A.
6. The biosensor system of claim 1 , wherein the mutation confers improved selectivity for detecting erythromycin A in comparison to other polyketides.
7. A genetically modified host cell comprising:
a nucleic acid encoding the biosensor of claim 1 .
8. A method for detecting a polyketide, comprising:
introducing into a cell:
i. a nucleic acid encoding a genetically modified MphR gene sequence, wherein the nucleic acid comprises at least one genetic mutation when compared to the wild-type MphR gene sequence; and
ii. a reporter gene whose transcription is under the control of a promoter region which is regulated by a MphR transcription factor; and
detecting the polyketide based on the differential expression of the reporter gene in comparison to a cell comprising a wild-type MphR gene sequence;
wherein the MphR genetic mutation encodes an amino acid change selected from T17R, T27G, T49I, 065M, T27A, M59E, M59S, R22H, K35N, T49I, L89V, D98N, E109D, R122T, K132N, A151T, H184Q, or a combination thereof, in the ligand binding domain.