IP Library › Granted Patent US 12,529,064
Granted Patent B2
US 12,529,064 · App. 18/057,190 · Granted Jan 20, 2026

Transgenic plants with increased photosynthesis efficiency and growth

Inventors: Stephen P. Long (Urbana, IL); Johannes Kromdijk (Cambridge, GB); Katarzyna Glowacka (Lincoln, NE); Krishna K. Niyogi (Oakland, CA); Laurie Beth Leonelli (Oakland, CA); Stephane T. Gabilly (Walnut Creek, CA)
Assignees: The Board of Trustees of the University of Illinois; The Regents of the University of California
C12N15/8269C12Y110/99003C12Y114/1309C07K14/415C12N9/0073
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Quick Facts
Patent No.
US 12,529,064
App. No.
18/057,190
Granted
Jan 20, 2026
Kind
B2
Abstract

The present disclosure provides a transgenic plant comprising one or more nucleotide sequences encoding polypeptides selected from photosystem II subunit S (PsbS), zeaxanthin epoxidase (ZEP), and violaxanthin de-epoxidase (VDE), operably linked to at least one expression control sequence. Expression vectors for making transgenic plants, and methods for increasing biomass production and/or carbon fixation and/or growth in a plant comprising increasing expression of at least one of PsbS, ZEP and VDE polypeptides are also provided.

Claims (91)

1 . A genetically modified higher plant comprising genetic modifications that increase expression of both a zeaxanthin epoxidase (ZEP) polypeptide and a violaxanthin de-epoxidase (VDE) polypeptide as compared to expression of the ZEP polypeptide and the VDE polypeptide in a control plant without the genetic modifications grown under the same conditions, wherein the genetically modified higher plant is a tracheophyte, wherein the genetically modified plant comprises at least endogenous levels of an endogenous photosystem II subunit S (PsbS) polypeptide, wherein the expression of the VDE polypeptide is increased by expressing a transfected nucleotide sequence encoding the VDE polypeptide in the genetically modified plant and/or by a genetic modification in a promoter of a nucleotide sequence encoding the VDE polypeptide, and wherein the expression of the ZEP polypeptide is increased by expressing a transfected nucleotide sequence encoding the ZEP polypeptide in the genetically modified plant and/or by a genetic modification in a promoter of a nucleotide sequence encoding the ZEP polypeptide.

2 . The genetically modified higher plant of claim 1 , wherein the expression of the VDE polypeptide is increased by expressing the transfected nucleotide sequence encoding the VDE polypeptide, and wherein the transfected nucleotide sequence is operably linked to at least one expression control sequence; and wherein the expression of the ZEP polypeptide is increased by expressing the transfected nucleotide sequence encoding the ZEP polypeptide, and wherein the transfected nucleotide sequence is operably linked to at least one expression control sequence.

3 . The genetically modified higher plant of claim 1 , wherein one or both of the transfected nucleotide sequences are derived from a dicot or a monocot.

4 . The genetically modified higher plant of claim 3 , wherein the dicot is selected from Arabidopsis thaliana, Beta vulgaris (sugar beet), Glycine max (soybean), Vigna unguiculata (cowpea), and Manihot esculenta (cassava), or wherein the monocot comprises Saccharum officinarum (sugarcane).

5 . The genetically modified higher plant of claim 1 , wherein:

(a) the ZEP polypeptide is encoded by a nucleotide sequence having at least 70% identity to SEQ ID NO: 2;

the ZEP polypeptide has an amino acid sequence having at least 70% identity to SEQ ID NO: 5, KRH28470.1, NP_001241348.1, or XP_010666612.1; or

the ZEP polypeptide comprises an amino acid sequence having at least 70% identity to SEQ ID NO: 8; and/or

(b) the VDE polypeptide is encoded by the nucleotide sequence having at least 70% identity to SEQ ID NO: 3 or a conserved domain thereof;

the VDE polypeptide has an amino acid sequence having at least 70% identity to SEQ ID NO: 6, SEQ ID NO: 67 (XP_021623226.1), XP_006576259.1, XP_014628869.1, XP_014628868.1, NP_001241404.1, NP_001240949.1, XP_006576257.1, XP_010674199.1, or a conserved domain thereof; or

the VDE polypeptide comprises a conserved domain having at least 70% identity to SEQ ID NO: 9 or a conserved domain thereof.

6 . The genetically modified higher plant of claim 5 , wherein:

(a) the ZEP is encoded by the nucleotide sequence having at least 80%, 90%, or 100% identity to SEQ ID NO: 2;

the ZEP polypeptide has an amino acid sequence having at least 80%, 90%, or 100% identity to SEQ ID NO: 5, KRH28470.1, NP_001241348.1, or XP_010666612.1; or

the ZEP polypeptide comprises an amino acid sequence having at least 80%, 90%, or 100% identity to SEQ ID NO: 8; and/or

(b) the VDE polypeptide is encoded by the nucleotide sequence having at least 80%, 90%, or 100% identity to SEQ ID NO: 3 or a conserved domain thereof;

the VDE polypeptide has an amino acid sequence having at least 80%, 90%, or 100% identity to SEQ ID NO: 6, SEQ ID NO: 67 (XP_021623226.1), XP_006576259.1, XP_014628869.1, XP_014628868.1, NP_001241404.1, NP_001240949.1, XP_006576257.1, XP_010674199.1, or a conserved domain thereof; or

the VDE polypeptide comprises a conserved domain having at least 80%, 90%, or 100% identity to SEQ ID NO: 9 or a conserved domain thereof.

7 . The genetically modified higher plant of claim 1 , wherein:

the ZEP is encoded by a nucleotide sequence of SEQ ID NO: 2, and the VDE polypeptide is encoded by a nucleotide sequence of SEQ ID NO: 3;

the ZEP polypeptide is encoded by a nucleotide sequence having at least 90% identity to SEQ ID NO: 2, and the VDE polypeptide is encoded by a nucleotide sequence having at least 90% identity to SEQ ID NO: 3;

the ZEP polypeptide is encoded by a nucleotide sequence having at least 70% identity to SEQ ID NO: 2, and the VDE polypeptide is encoded by a nucleotide sequence having at least 70% identity to SEQ ID NO: 3;

the ZEP polypeptide has an amino acid sequence of SEQ ID NO: 5, and the VDE polypeptide has an amino acid sequence of SEQ ID NO: 6;

the ZEP polypeptide has an amino acid sequence at least 90% identical to SEQ ID NO: 5, and the VDE polypeptide has an amino acid sequence at least 90% identical to SEQ ID NO: 6;

the ZEP polypeptide has an amino acid sequence at least 70% identical to SEQ ID NO: 5, and the VDE polypeptide has an amino acid sequence at least 70% identical to SEQ ID NO: 6; and/or

the ZEP polypeptide comprises the amino acid sequence of SEQ ID NO: 8, and the VDE polypeptide comprises a conserved domain of SEQ ID NO: 9.

8 . The genetically modified higher plant of claim 1 , wherein:

the ZEP polypeptide has the amino acid sequence of KRH28470.1 or NP_001241348.1, and the VDE polypeptide has the amino acid sequence of XP_006576259.1, XP_014628869.1, XP_014628868.1, NP_001241404.1, NP_001240949.1, or XP_006576257.1;

the ZEP polypeptide has an amino acid sequence at least 90% identical to KRH28470.1 or NP 001241348.1, and the VDE polypeptide has an amino acid sequence at least 90% identical to XP_006576259.1, XP_014628869.1, XP_014628868.1, NP_001241404.1, NP_001240949.1, or XP 006576257.1;

the ZEP polypeptide has an amino acid sequence at least 70% identical to KRH28470.1 or NP 001241348.1, and the VDE polypeptide has an amino acid sequence at least 70% identical to XP 006576259.1, XP_014628869.1, XP_014628868.1, NP_001241404.1, NP_001240949.1, or XP 006576257.1;

the ZEP polypeptide comprises the amino acid sequence of KRH28470.1 or NP_001241348.1, and the VDE polypeptide comprises a conserved domain of XP_006576259.1, XP_014628869.1, XP_014628868.1, NP_001241404.1, NP_001240949.1, or XP_006576257.1;

the ZEP polypeptide has the amino acid sequence of XP_010666612.1, and the VDE polypeptide has the amino acid sequence of XP_010674199.1;

the ZEP polypeptide has an amino acid sequence at least 90% identical to XP_010666612.1, and the VDE polypeptide has an amino acid sequence at least 90% identical to XP_010674199.1;

the ZEP polypeptide has an amino acid sequence at least 70% identical to XP_010666612.1 or NP_001241348.1, and the VDE polypeptide has an amino acid sequence at least 70% identical to XP 010674199.1;

the ZEP polypeptide comprises the amino acid sequence of XP_010666612.1, and the VDE polypeptide comprises a conserved domain of XP_010674199.1;

the VDE polypeptide has the amino acid sequence of SEQ ID NO: 67;

the VDE polypeptide has an amino acid sequence at least 90% identical to SEQ ID NO: 67;

the VDE polypeptide has an amino acid sequence at least 70% identical to SEQ ID NO: 67; and/or

the VDE polypeptide comprises a conserved domain of SEQ ID NO: 67.

9 . The genetically modified higher plant of claim 8 , further comprising a genetic modification that increases expression of a photosystem II subunit S (PsbS) polypeptide as compared to expression of the endogenous PsbS polypeptide in a control plant without the genetic modification grown under the same conditions, wherein the expression of the PsbS polypeptide is increased by expressing a transfected nucleotide sequence encoding the PsbS polypeptide in the genetically modified plant and/or by a genetic modification in a promoter of a nucleotide sequence encoding the endogenous PsbS polypeptide, and wherein

the PsbS polypeptide is encoded by the nucleotide sequence of SEQ ID NO: 1;

the PsbS polypeptide is encoded by a nucleotide sequence at least 90% identical to SEQ ID NO: 1;

the PsbS polypeptide is encoded by a nucleotide sequence at least 70% identical to SEQ ID NO: 1;

the PsbS polypeptide has the amino acid sequence of SEQ ID NO: 4;

the PsbS polypeptide has an amino acid sequence at least 90% identical to SEQ ID NO: 4;

the PsbS polypeptide has an amino acid sequence at least 70% identical to SEQ ID NO: 4; and/or

the PsbS polypeptide comprises a conserved domain of SEQ ID NO: 1, SEQ ID NO: 4, or SEQ ID NO: 7; the PsbS polypeptide has an amino acid sequence of XP_003523444.1, NP_001276237.1, or ACU23291.1;

the PsbS polypeptide has an amino acid sequence at least 90% identical to XP_003523444.1, NP_001276237.1, or ACU23291.1;

the PsbS polypeptide has an amino acid sequence at least 70% identical to XP_003523444.1, NP_001276237.1, or ACU23291.1;

the PsbS polypeptide comprises a conserved domain of SEQ ID NO: 4, XP_003523444.1, NP_001276237.1, or ACU23291.1;

the PsbS polypeptide has an amino acid sequence of SEQ ID NO: 28;

the PsbS polypeptide has an amino acid sequence at least 90% identical to SEQ ID NO: 28;

the PsbS polypeptide has an amino acid sequence at least 70% identical to SEQ ID NO: 28; and/or

the PsbS polypeptide comprises a conserved domain of SEQ ID NO: 28.

10 . The genetically modified higher plant of claim 9 , wherein the expression of the PsbS polypeptide is increased by expressing the transfected nucleotide sequence encoding the PsbS polypeptide, and wherein the transfected nucleotide sequence is operably linked to at least one expression control sequence.

11 . The genetically modified higher plant of claim 1 , wherein the plant is a crop plant, a model plant, a monocotyledonous plant, a dicotyledonous plant, a plant with Crassulacean acid metabolism (CAM) photosynthesis, a plant with C3 photosynthesis, a plant with C4 photosynthesis, an annual plant, a greenhouse plant, a horticultural flowering plant, a perennial plant, a switchgrass plant, a maize plant, a biomass plant, or a sugarcane plant.

12 . The genetically modified higher plant of claim 1 , wherein the plant is selected from the group of tobacco, corn, rice, sorghum, soybean, cowpea, poplar, eucalyptus, cassava, barley, potato, sugarcane, alfalfa, sugar beet, or wherein the plant is selected from the group consisting of switchgrass, Miscanthus, Medicago, sweet sorghum, grain sorghum, sugarcane, energy cane, elephant grass, maize, cassava, cowpea, wheat, barley, oats, rice, soybean, sugar beet, oil palm, safflower, sesame, tobacco, flax, cotton, sunflower, Camelina, Brassica napus, Brassica carinata, Brassica juncea , pearl millet, foxtail millet, other grain, rice, oilseed, a vegetable crop, a forage crop, an industrial crop, a woody crop and a biomass crop.

13 . The genetically modified higher plant of claim 12 , wherein the plant is:

sugar beet, the ZEP polypeptide has an amino acid sequence at least 70% identical to XP 010666612.1 or NP_001241348.1, the VDE polypeptide has an amino acid sequence at least 70% identical to XP_010674199.1;

soybean, the ZEP polypeptide has an amino acid sequence at least 70% identical to KRH28470.1 or NP_001241348.1, the VDE polypeptide has an amino acid sequence at least 70% identical to XP_006576259.1, XP_014628869.1, XP_014628868.1, NP_001241404.1, NP_001240949.1, or XP_006576257.1;

cassava and the VDE polypeptide has an amino acid sequence at least 70% identical to SEQ ID NO: 67;

cowpea; or

sugarcane.

14 . The genetically modified higher plant of claim 1 , wherein:

the plant has improved growth under fluctuation light conditions as compared to the control plant grown under the same fluctuating light conditions;

the plant has increased photosynthetic efficiency under fluctuating light conditions as compared to the control plant grown under the same fluctuating light conditions;

the plant has improved photoprotection efficiency under fluctuating light conditions as compared to the control plant grown under the same fluctuating light conditions;

the plant has an increased rate of relaxation of non-photochemical quenching (NPQ) under fluctuating light conditions as compared to the control plant grown under the same fluctuating light conditions; and/or

the plant has improved quantum yield and CO 2 fixation under fluctuating light conditions as compared to the control plant grown under the same fluctuating light conditions.

15 . The genetically modified higher plant of claim 1 , wherein the plant further comprises expression of at least one additional polypeptide that provides herbicide resistance, insect or pest resistance, disease resistance, modified fatty acid metabolism, and/or modified carbohydrate metabolism.

16 . The genetically modified higher plant of claim 1 , wherein:

the transcript level of the VDE polypeptide is increased 3-fold as compared to the control plant, and wherein the transcript level of the ZEP polypeptide is increased 8-fold as compared to the control plant;

the transcript level of the VDE polypeptide is increased 10-fold as compared to the control plant, and wherein the transcript level of the ZEP polypeptide is increased 6-fold as compared to the control plant;

the transcript level of the VDE polypeptide is increased 4-fold as compared to the control plant, and wherein the transcript level of the ZEP polypeptide is increased 7-fold as compared to the control plant;

the protein level of the VDE polypeptide is increased 16-fold as compared to the control plant, and wherein the protein level of the ZEP polypeptide is increased 80-fold as compared to the control plant;

the protein level of the VDE polypeptide is increased 30-fold as compared to the control plant, and wherein the protein level of the ZEP polypeptide is increased 74-fold as compared to the control plant;

the protein level of the VDE polypeptide is increased 47-fold as compared to the control plant, and wherein the protein level of the ZEP polypeptide is increased 75-fold as compared to the control plant;

the increase of transcript level as compared to the control plant between the VDE polypeptide and the ZEP polypeptide has a ratio selected from the group consisting of 3:8, 10:6, and 4:7;

the increase of protein level as compared to the control plant between the VDE polypeptide and the ZEP polypeptide has a ratio selected from the group consisting of 16:80, 30:74, and 47:75;

the increase of transcript level of the VDE polypeptide as compared to the control plant is from about 3-fold to about 10-fold, and wherein the increase of transcript level of the ZEP polypeptide as compared to the control plant is from about 6-fold to about 8-fold; or

the increase of protein level of the VDE polypeptide as compared to the control plant is in from about 16-fold to about 47-fold, and wherein the increase of protein level of the ZEP polypeptide as compared to the control plant is from about 74-fold to about 80-fold.

17 . The genetically modified higher plant of claim 5 , further comprising a genetic modification that increases expression of a photosystem II subunit S (PsbS) polypeptide as compared to expression of the endogenous PsbS polypeptide in a control plant without the genetic modification grown under the same conditions, wherein the expression of the PsbS polypeptide is increased by expressing a transfected nucleotide sequence encoding the PsbS polypeptide in the genetically modified plant and/or by a genetic modification in a promoter of a nucleotide sequence encoding the endogenous PsbS polypeptide, wherein the PsbS polypeptide:

(i) is encoded by a nucleotide sequence having at least 70% identity to SEQ ID NO: 1 or a conserved domain thereof;

(ii) has an amino acid sequence having at least 70% identity to SEQ ID NO: 4, XP_003523444.1, NP_001276237.1, ACU23291.1, SEQ ID NO: 28 (XP_010692414.1), or a conserved domain thereof; or

(iii) comprises a conserved domain having at least 70% identity to SEQ ID NO: 7 or a conserved domain thereof.

18 . The genetically modified higher plant of claim 17 , wherein the PsbS polypeptide:

(i) is encoded by a nucleotide sequence having at least 80%, 90%, or 100% identity to SEQ ID NO: 1 or a conserved domain thereof;

(ii) has an amino acid sequence having at least 80%, 90%, or 100% identity to SEQ ID NO: 4, XP_003523444.1, NP_001276237.1, ACU23291.1, SEQ ID NO: 28 (XP_010692414.1), or a conserved domain thereof; or

(iii) comprises a conserved domain having at least 80%, 90%, or 100% identity to SEQ ID NO: 7 or a conserved domain thereof.

19 . The genetically modified higher plant of claim 13 , wherein the plant is sugar beet, and wherein the plant further comprises a genetic modification that increases expression of a PsbS polypeptide as compared to expression of the endogenous PsbS polypeptide in a control plant without the genetic modification grown under the same conditions, wherein the expression of the PsbS polypeptide is increased by expressing a transfected nucleotide sequence encoding the PsbS polypeptide in the genetically modified plant and/or by a genetic modification in a promoter of a nucleotide sequence encoding the endogenous PsbS polypeptide, and the PsbS polypeptide has an amino acid sequence at least 70% identical to SEQ ID NO: 28.

20 . The genetically modified higher plant of claim 13 , wherein the plant is soybean, and wherein the plant further comprises a genetic modification that increases expression of a PsbS polypeptide as compared to expression of the endogenous PsbS polypeptide in a control plant without the genetic modification grown under the same conditions, wherein the expression of the PsbS polypeptide is increased by expressing a transfected nucleotide sequence encoding the PsbS polypeptide in the genetically modified plant and/or by a genetic modification in a promoter of a nucleotide sequence encoding the endogenous PsbS polypeptide, and the PsbS polypeptide has an amino acid sequence at least 70% identical to XP_003523444.1, NP_001276237.1, or ACU23291.1.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 26, 2024
From: LONG, STEPHEN P.; KROMDIJK, JOHANNES; GLOWACKA, KATARZYNA
To: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
Reel/Frame 066266/0253 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 26, 2024
From: HOWARD HUGHES MEDICAL INSTITUTE
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 066266/0256 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 26, 2024
From: NIYOGI, KRISHNA K.; LEONELLI, LAURIE; GABILLY, STÉPHANE T.
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 066266/0262 →
Continuity (3)
Continuation 16304633
Provisional Application 62342248 · May 27, 2016
Related Publication 20230183731A1 · Jun 15, 2023
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