METHODS OF CAPTURING CELL-FREE METHYLATED DNA AND USES OF SAME
There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
1 . A method of capturing cell-free methylated DNA from a sample having less than 100 ng of cell-free DNA, comprising the steps of:
subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA;
adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated;
denaturing the sample; and
capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
2 . The method of claim 1 further comprising the step of amplifying and subsequently sequencing the captured cell-free methylated DNA.
3 . The method of claim 1 , wherein the sample has less than 50 ng of cell-free DNA.
4 . The method of claim 1 , wherein the first amount of filler DNA comprises about 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% methylated filler DNA with remainder being unmethylated filler DNA, and preferably between 5% and 50%, between 10%40%, or between 15%-30% methylated filler DNA.
5 . The method of claim 1 , wherein the first amount of filler DNA is from 20 ng to 100 ng, preferably 30 ng to 100 ng, more preferably 50 ng to 100 ng.
6 . The method of claim 1 , wherein the cell-free DNAfrom the sample and the first amount of filler DNA together comprises at least 50 ng of total DNA, preferably at least 100 ng of total DNA.
7 . The method of claim 1 , wherein the filler DNA is 50 bp to 800 bp long, preferably 100 bp to 600 bp long, and more preferably 200 bp to 600 bp long.
8 . The method of claim 1 , wherein the filler DNA is double stranded.
9 . The method of claim 1 , wherein the filler DNA is junk DNA.
10 . The method of claim 1 , wherein the filler DNA is endogenous or exogenous DNA.
11 . The method of claim 10 , wherein the filler DNA is non-human DNA, preferably X, DNA.
12 . The method of claim 1 , wherein the filler DNA has no alignment to human DNA.
13 . The method of claim 1 , wherein the binder is a protein comprising a Methyl-CpG-binding domain.
14 . The method of claim 13 , wherein the protein is a MBD2 protein.
15 . The method of claim 1 , wherein step (d) comprises immunoprecipitating the cell-free methylated DNA using an antibody.
16 . The method of claim 15 , comprising adding at least 0.05 µg of the antibody to the sample for immunoprecipitation, and preferably at least 0.16 µg.
17 . The method of claim 15 , wherein the antibody is 5-MeC antibody or 5-hydroxymethyl cytosine antibody.
18 . The method of claim 15 , further comprising the step of adding a second amount of control DNA to the sample after step (b) for confirming the immunoprecipitation reaction.
19 . The method of claim 1 , further comprising the step of adding a second amount of control DNA to the sample after step (b) for confirming the capture of cell-free methylated DNA.
20 . A method of measuring a DNA methylation profile within the sample comprising the method of claim 1 .