Fluorescence-coded mid-infrared photothermal microscope
Microscopic analysis of a sample includes a fluorescent dye disposed within the sample. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. An optical source generates a probe beam directed to impinge on the sample. A detector detects fluorescent emissions from the sample when the probe beam impinges on the sample. A data acquisition and processing system acquires and processes the detected fluorescent emissions from the sample to: (i) generate a signal indicative of infrared absorption by the sample, (ii) generate a signal indicative of temperature in the sample based on the signal indicative of infrared absorption by the sample, (iii) generate an image of the sample using the signal indicative of temperature in the sample.
1. A system for microscopic analysis of a sample with at least one fluorescence label, comprising:
a mid-infrared (IR) optical source for generating a mid-infrared beam, the mid-infrared beam being directed onto at least a portion of the sample to induce a temperature change in the portion of the sample by absorption of the mid-infrared beam;
an optical source for generating a probe beam, the probe beam being directed to impinge on the sample;
a detector for detecting fluorescent emissions from the sample when the probe beam impinges on the sample; and
a computer and/or processor for acquiring and processing the detected fluorescent emissions from the sample to generate a signal indicative of infrared absorption of the sample, due to temperature-induced changes in fluorescent emission from the at least one fluorescent label.
2. The system of claim 1 , wherein the sample comprises at least one of bacteria and cancer cells.
3. The system of claim 1 , wherein the detector is a camera.
4. The system of claim 1 , wherein the optical source that generates a probe beam is pulsed.
5. The system of claim 3 , further comprising at least one pulse generator to synchronize at least two of: IR pump pulses, probe pulses, and camera exposure.
6. The system of claim 1 , wherein the sample comprises a liquid supplemented with at least one thermo-sensitive fluorescent dye.
7. The system of claim 1 , wherein the at least one fluorescent label comprises at least one of rhodamine B, fluorescein, cy2, cy3, Nile red and green fluorescent protein.
8. The system of claim 1 , wherein the signal indicative of infrared absorption of the sample is measured at a plurality of locations on the sample to form an image indicative of infrared absorption over the plurality of locations.
9. The system of claim 3 , wherein photobleaching of the at least one fluorophore is less than 5% over fifty image frames of the camera.
10. The system of claim 1 , wherein optical power directed to impinge on the sample from at least one of the mid-infrared (IR) optical source and the probe beam optical source is microwatt-level.
11. The system of claim 1 , wherein the probe beam probe beam impinging on the sample has an optical power density of less than 10 kW/cm2.
12. The system of claim 3 , wherein the computer and/or processor calculates a difference between hot and cold frames from the camera to produce a difference image indicative of infrared absorption of the sample.
13. The system of claim 12 , wherein the image indicative of infrared absorption of the sample has a signal-to-noise ratio of greater than or equal to ten.
14. The system of claim 12 , wherein difference images are acquired at greater than or equal to twenty frames per second.
15. The system of claim 12 , wherein the difference image has a spatial resolution of <500 nm.