Continuous analyte monitoring system with microneedle array
Described herein are variations of an analyte monitoring system, including an analyte monitoring device. For example, an analyte monitoring device may include an implantable microneedle array for use in measuring one or more analytes (e.g., glucose), such as in a continuous manner. The microneedle array may include, for example, at least one microneedle including a tapered distal portion having an insulated distal apex, and an electrode on a surface of the tapered distal portion located proximal to the insulated distal apex. At least some of the microneedles may be electrically isolated such that one or more electrodes is individually addressable.
1. A microneedle array for use in sensing an analyte, comprising:
a first solid, lumen-free microneedle comprising a working electrode, wherein the working electrode comprises a first electrode material and a biorecognition layer on the first electrode material, wherein the biorecognition layer comprises a biorecognition element configured to react with the analyte, wherein a distal tip of the first solid, lumen-free microneedle is insulated;
a second, lumen-free solid microneedle comprising a counter electrode, wherein the counter electrode comprises a second electrode material; and
a third solid, lumen-free microneedle comprising a reference electrode, wherein the reference electrode comprises a third electrode material and a redox-couple layer on the third electrode material, and
wherein a redox reaction occurs between the working electrode on the first solid, lumen-free microneedle and the counter electrode on the second solid, lumen-free microneedle.
2. The microneedle array of claim 1 , wherein the working electrode further comprises an electrocatalytic layer between the first electrode material and the biorecognition layer.
3. The microneedle array of claim 2 , wherein the electrocatalytic layer is a platinum black layer, and wherein the platinum black layer comprises elemental platinum metal.
4. The microneedle array of claim 2 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer.
5. The microneedle array of claim 1 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer.
6. The microneedle array of claim 1 , wherein the working electrode further comprises a hydrophilic layer.
7. The microneedle array of claim 1 , wherein the biorecognition layer is configured to both facilitate selective analyte quantification utilizing the biorecognition element and block interferents.
8. The microneedle array of claim 1 , wherein the biorecognition element comprises one or more enzymes selected from the group consisting of: glucose oxidase, lactate oxidase, alcohol oxidase, beta-hydroxybutyrate dehydrogenase, tyrosinase, catalase, ascorbate oxidase, cholesterol oxidase, choline oxidase, pyruvate oxidase, urate oxidase, urease, and xanthine oxidase.
9. The microneedle array of claim 1 , wherein the biorecognition element comprises at least one polymer selected from the group consisting of: aniline, pyrrole, acetylene, phenylene, phenylene vinylene, phenylene diamine, thiophene, 3,4-ethylenedioxythiophene, and aminophenylboronic acid.
10. The microneedle array of claim 1 , wherein the first electrode material and the second electrode material are different from the third electrode material.
11. The microneedle array of claim 1 , wherein one or more of the first electrode material and the second electrode material is a material selected from the group consisting of: platinum, palladium, iridium, rhodium, gold, ruthenium, titanium, nickel, carbon, and doped diamond.
12. The microneedle array of claim 1 , wherein the first electrode material and the second electrode material are the same material.
13. The microneedle array of claim 1 , wherein the third electrode material is a metal salt or a metal oxide selected from the group consisting of: silver-silver chloride and iridium oxide.
14. The microneedle array of claim 1 , wherein the working electrode is annular and is on only a segment of a surface of the first solid, lumen-free microneedle.
15. The microneedle array of claim 14 , wherein a distal edge of the annular working electrode is offset from the insulated distal tip of the first solid, lumen-free microneedle and is proximate a proximal edge of the insulated distal tip.
16. The microneedle array of claim 1 , wherein the working electrode is disposed around a perimeter of the first solid, lumen-free microneedle.
17. The microneedle array of claim 1 , wherein the reference electrode further comprises an electrocatalytic layer on the third electrode material.
18. A microneedle array for use in sensing glucose, comprising:
a first solid microneedle comprising a working electrode, wherein the working electrode comprises a platinum layer and a biorecognition layer on the platinum layer, wherein the biorecognition layer comprises a conducting polymer-entrapped glucose oxidase configured to react with the glucose, wherein a distal tip of the first solid microneedle is insulated;
a second solid microneedle comprising a counter electrode, wherein the counter electrode comprises a platinum layer; and
a third solid microneedle comprising a reference electrode, wherein the reference electrode comprises silver/silver chloride, and
wherein a redox reaction occurs between the working electrode on the first solid microneedle and the counter electrode on the second solid microneedle.
19. The microneedle array of claim 18 , wherein the working electrode further comprises an electrocatalytic layer between the platinum layer and the biorecognition layer.
20. The microneedle array of claim 19 , wherein the electrocatalytic layer is a platinum black layer, and wherein the platinum black layer comprises elemental platinum metal.
21. The microneedle array of claim 19 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer.
22. The microneedle array of claim 18 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer.
23. The microneedle array of claim 18 , wherein the working electrode further comprises a hydrophilic layer.
24. The microneedle array of claim 18 , wherein the biorecognition layer is configured to both facilitate selective analyte quantification utilizing the biorecognition element and block interferents.
25. The microneedle array of claim 18 , wherein the biorecognition element comprises at least one polymer selected from the group consisting of: aniline, pyrrole, acetylene, phenylene, phenylene vinylene, phenylene diamine, thiophene, 3,4-ethylenedioxythiophene, and aminophenylboronic acid.
26. The microneedle array of claim 18 , wherein the working electrode is annular and is on only a segment of a surface of the first solid microneedle.
27. The microneedle array of claim 26 , wherein a distal edge of the annular working electrode is offset from the insulated distal tip of the first solid microneedle and is proximate a proximal edge of the insulated distal tip.
28. The microneedle array of claim 18 , wherein the working electrode is disposed around a perimeter of the first solid microneedle.
29. A method for sensing an analyte via a microneedle array, comprising:
providing a first solid, lumen-free microneedle comprising a working electrode, wherein the working electrode comprises a first electrode material and a biorecognition layer on the first electrode material, wherein the biorecognition layer comprises a biorecognition element configured to react with the analyte, wherein a distal tip of the first solid, lumen-free microneedle is insulated;
providing a second solid, lumen-free microneedle comprising a counter electrode, wherein the counter electrode comprises a second electrode material;
providing a third solid, lumen-free microneedle comprising a reference electrode, wherein the reference electrode comprises a third electrode material and a redox-couple layer on the third electrode material;
applying a bias potential between the working electrode and the reference electrode; and
measuring a current value between the working electrode and the counter electrode, the current value being proportional to a concentration of the analyte,
wherein a redox reaction occurs between the working electrode and the counter electrode.
30. The method of claim 29 , wherein the working electrode further comprises an electrocatalytic layer between the first electrode material and the biorecognition layer.
31. The method of claim 30 , wherein the electrocatalytic layer is a platinum black layer, and wherein the platinum black layer comprises elemental platinum metal.
32. The method of claim 29 , wherein the biorecognition element comprises one or more enzymes selected from the group consisting of: glucose oxidase, lactate oxidase, alcohol oxidase, beta-hydroxybutyrate dehydrogenase, tyrosinase, catalase, ascorbate oxidase, cholesterol oxidase, choline oxidase, pyruvate oxidase, urate oxidase, urease, and xanthine oxidase.
33. The method of claim 29 , wherein the biorecognition element comprises at least one of a polymer selected from the group consisting of: aniline, pyrrole, acetylene, phenylene, phenylene vinylene, phenylene diamine, thiophene, 3,4-ethylenedioxythiophene, and aminophenylboronic acid.
34. The method of claim 29 , wherein the working electrode is annular and is on only a segment of a surface of the first solid, lumen-free microneedle.
35. A method for sensing glucose via a microneedle array, comprising:
providing a first solid microneedle comprising a working electrode, wherein the working electrode comprises a platinum layer and a biorecognition layer on the platinum layer, wherein the biorecognition layer comprises a conducting polymer-entrapped glucose oxidase configured to react with the glucose, wherein a distal tip of the first solid microneedle is insulated;
providing a second solid microneedle comprising a counter electrode, wherein the counter electrode comprises a platinum layer;
providing a third solid microneedle comprising a reference electrode, wherein the reference electrode comprises silver/silver chloride;
applying a bias potential between the working electrode and the reference electrode; and
measuring a current value between the working electrode and the counter electrode, the current value being proportional to a concentration of the glucose,
wherein a redox reaction occurs between the working electrode on the first solid microneedle and the counter electrode on the second solid microneedle.
36. The method of claim 35 , wherein the working electrode further comprises an electrocatalytic layer between the platinum layer and the biorecognition layer.
37. The method of claim 36 , wherein the electrocatalytic layer is a platinum black layer, and wherein the platinum black layer comprises elemental platinum metal.
38. The method of claim 35 , wherein the biorecognition element comprises at least one of a polymer selected from the group consisting of: aniline, pyrrole, acetylene, phenylene, phenylene vinylene, phenylene diamine, thiophene, 3,4-ethylenedioxythiophene, and aminophenylboronic acid.
39. The method of claim 35 , wherein the working electrode is annular and is on only a segment of a surface of the first solid microneedle.
40. A microneedle array for use in sensing an analyte, comprising:
a first solid microneedle comprising a working electrode, wherein the working electrode comprises a first electrode material and a biorecognition layer on the first electrode material, wherein the biorecognition layer comprises a biorecognition element configured to react with the analyte;
a second solid microneedle comprising a counter electrode, wherein the counter electrode comprises a second electrode material and an electrocatalytic layer on the second electrode material; and
a third solid microneedle comprising a reference electrode, wherein the reference electrode comprises a third electrode material and a redox-couple layer on the third electrode material, and
wherein a redox reaction occurs between the working electrode on the first solid microneedle and the counter electrode on the second solid microneedle.
41. The microneedle array of claim 40 , wherein the working electrode further comprises an electrocatalytic layer between the first electrode material and the biorecognition layer.
42. The microneedle array of claim 41 , wherein the electrocatalytic layer is a platinum black layer, and wherein the platinum black layer comprises elemental platinum metal.
43. The microneedle array of claim 41 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer.
44. The microneedle array of claim 40 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer.
45. The microneedle array of claim 40 , wherein the working electrode further comprises a hydrophilic layer.
46. The microneedle array of claim 40 , wherein the biorecognition layer is configured to both facilitate selective analyte quantification utilizing the biorecognition element and block interferents.
47. The microneedle array of claim 40 , wherein the biorecognition element comprises one or more enzymes selected from the group consisting of: glucose oxidase, lactate oxidase, alcohol oxidase, beta-hydroxybutyrate dehydrogenase, tyrosinase, catalase, ascorbate oxidase, cholesterol oxidase, choline oxidase, pyruvate oxidase, urate oxidase, urease, and xanthine oxidase.
48. The microneedle array of claim 40 , wherein the biorecognition element comprises at least one polymer selected from the group consisting of: aniline, pyrrole, acetylene, phenylene, phenylene vinylene, phenylene diamine, thiophene, 3,4-ethylenedioxythiophene, and aminophenylboronic acid.
49. The microneedle array of claim 40 , wherein the first electrode material and the second electrode material are different from the third electrode material.
50. The microneedle array of claim 40 , wherein one or more of the first electrode material and the second electrode material is a material selected from the group consisting of: platinum, palladium, iridium, rhodium, gold, ruthenium, titanium, nickel, carbon, and doped diamond.
51. The microneedle array of claim 40 , wherein the first electrode material and the second electrode material are the same material.
52. The microneedle array of claim 40 , wherein the third electrode material is a metal salt or a metal oxide selected from the group consisting of: silver-silver chloride and iridium oxide.
53. The microneedle array of claim 40 , wherein the working electrode is annular and is on only a segment of a surface of the first solid microneedle.
54. The microneedle array of claim 53 , wherein a distal tip of the first solid microneedle is insulated and a distal edge of the annular working electrode is offset from the insulated distal tip and proximate a proximal edge of the insulated distal tip.
55. The microneedle array of claim 40 , wherein the working electrode is disposed around a perimeter of the first solid microneedle.
56. The microneedle array of claim 40 , wherein the reference electrode further comprises an electrocatalytic layer on the third electrode material.
57. A method for sensing an analyte via a microneedle array, comprising:
providing a first solid microneedle comprising a working electrode, wherein the working electrode comprises a first electrode material and a biorecognition layer on the first electrode material, wherein the biorecognition layer comprises a biorecognition element configured to react with the analyte;
providing a second solid microneedle comprising a counter electrode, wherein the counter electrode comprises a second electrode material and an electrocatalytic layer on the second electrode material;
providing a third solid microneedle comprising a reference electrode, wherein the reference electrode comprises a third electrode material and a redox-couple layer on the third electrode material;
applying a bias potential between the working electrode and the reference electrode; and
measuring a current value between the working electrode and the counter electrode, the current value being proportional to a concentration of the analyte,
wherein a redox reaction occurs between the working electrode and the counter electrode.
58. The method of claim 57 , wherein the working electrode further comprises an electrocatalytic layer between the first electrode material and the biorecognition layer.
59. The method of claim 58 , wherein the electrocatalytic layer is a platinum black layer, and wherein the platinum black layer comprises elemental platinum metal.
60. The method of claim 57 , wherein the biorecognition element comprises one or more enzymes selected from the group consisting of: glucose oxidase, lactate oxidase, alcohol oxidase, beta-hydroxybutyrate dehydrogenase, tyrosinase, catalase, ascorbate oxidase, cholesterol oxidase, choline oxidase, pyruvate oxidase, urate oxidase, urease, and xanthine oxidase.
61. The method of claim 57 , wherein the biorecognition element comprises at least one of a polymer selected from the group consisting of: aniline, pyrrole, acetylene, phenylene, phenylene vinylene, phenylene diamine, thiophene, 3,4-ethylenedioxythiophene, and aminophenylboronic acid.
62. The method of claim 57 , wherein the working electrode is annular and is on only a segment of a surface of the first solid microneedle.
63. A microneedle array for use in sensing an analyte, comprising:
a first solid microneedle comprising a first body portion having a uniform composition, the first solid microneedle further comprising a working electrode, wherein the working electrode comprises a first electrode material and a biorecognition layer on the first electrode material, wherein the biorecognition layer comprises a biorecognition element configured to react with the analyte, wherein a distal tip of the first solid microneedle is insulated;
a second solid microneedle comprising a second body portion having a uniform composition, the second solid microneedle further comprising a counter electrode, wherein the counter electrode comprises a second electrode material; and
a third solid microneedle comprising a third body portion having a uniform composition, the third solid microneedle further comprising a reference electrode, wherein the reference electrode comprises a third electrode material and a redox-couple layer on the third electrode material, and
wherein a redox reaction occurs between the working electrode on the first solid microneedle and the counter electrode on the second solid microneedle.
64. The microneedle array of claim 63 , wherein the working electrode further comprises an electrocatalytic layer between the first electrode material and the biorecognition layer.
65. The microneedle array of claim 64 , wherein the electrocatalytic layer is a platinum black layer, and wherein the platinum black layer comprises elemental platinum metal.
66. The microneedle array of claim 64 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer.
67. The microneedle array of claim 63 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer.
68. The microneedle array of claim 63 , wherein the working electrode further comprises a hydrophilic layer.
69. The microneedle array of claim 63 , wherein the biorecognition layer is configured to both facilitate selective analyte quantification utilizing the biorecognition element and block interferents.
70. The microneedle array of claim 63 , wherein the biorecognition element comprises one or more enzymes selected from the group consisting of: glucose oxidase, lactate oxidase, alcohol oxidase, beta-hydroxybutyrate dehydrogenase, tyrosinase, catalase, ascorbate oxidase, cholesterol oxidase, choline oxidase, pyruvate oxidase, urate oxidase, urease, and xanthine oxidase.
71. The microneedle array of claim 63 , wherein the biorecognition element comprises at least one polymer selected from the group consisting of: aniline, pyrrole, acetylene, phenylene, phenylene vinylene, phenylene diamine, thiophene, 3,4-ethylenedioxythiophene, and aminophenylboronic acid.
72. The microneedle array of claim 63 , wherein the first electrode material and the second electrode material are different from the third electrode material.
73. The microneedle array of claim 63 , wherein one or more of the first electrode material and the second electrode material is a material selected from the group consisting of: platinum, palladium, iridium, rhodium, gold, ruthenium, titanium, nickel, carbon, and doped diamond.
74. The microneedle array of claim 63 , wherein the first electrode material and the second electrode material are the same material.
75. The microneedle array of claim 63 , wherein the third electrode material is a metal salt or a metal oxide selected from the group consisting of: silver-silver chloride and iridium oxide.
76. The microneedle array of claim 63 , wherein the working electrode is annular and is on only a segment of a surface of the first solid microneedle.
77. The microneedle array of claim 76 , wherein a distal edge of the annular working electrode is offset from the insulated distal tip of the first solid microneedle and is proximate a proximal edge of the insulated distal tip.
78. The microneedle array of claim 63 , wherein the working electrode is disposed around a perimeter of the first solid microneedle.
79. The microneedle array of claim 63 , wherein a portion of the first body portion, a portion of the second body portion, and a portion of the third body portion is surrounded by an insulative barrier.
80. The microneedle array of claim 63 , wherein the reference electrode further comprises an electrocatalytic layer on the third electrode material.
81. A method for sensing an analyte via a microneedle array, comprising:
providing a first solid microneedle comprising a first body portion having a uniform composition, the first solid microneedle further comprising a working electrode, wherein the working electrode comprises a first electrode material and a biorecognition layer on the first electrode material, wherein the biorecognition layer comprises a biorecognition element configured to react with the analyte, wherein a distal tip of the first solid microneedle is insulated;
providing a second solid microneedle comprising a second body portion having a uniform composition, the second solid microneedle further comprising a counter electrode, wherein the counter electrode comprises a second electrode material;
providing a third solid microneedle comprising a third body portion having a uniform composition, the third solid microneedle comprising a reference electrode, wherein the reference electrode comprises a third electrode material and a redox-couple layer on the third electrode material;
applying a bias potential between the working electrode and the reference electrode; and
measuring a current value between the working electrode and the counter electrode, the current value being proportional to a concentration of the analyte,
wherein a redox reaction occurs between the working electrode and the counter electrode.
82. The method of claim 81 , wherein the working electrode further comprises an electrocatalytic layer between the first electrode material and the biorecognition layer.
83. The method of claim 82 , wherein the electrocatalytic layer is a platinum black layer, and wherein the platinum black layer comprises elemental platinum metal.
84. The method of claim 81 , wherein the working electrode further comprises a working diffusion-limiting layer on the biorecognition layer, the counter electrode further comprises a counter diffusion-limiting layer on the second electrode material, and the reference electrode further comprises a reference diffusion-limiting layer on the third electrode material, and wherein the working diffusion-limiting material, the counter diffusion-limiting layer, and the reference diffusion-limiting layer comprise the same material.
85. The method of claim 81 , wherein the biorecognition element comprises one or more enzymes selected from the group consisting of: glucose oxidase, lactate oxidase, alcohol oxidase, beta-hydroxybutyrate dehydrogenase, tyrosinase, catalase, ascorbate oxidase, cholesterol oxidase, choline oxidase, pyruvate oxidase, urate oxidase, urease, and xanthine oxidase.
86. The method of claim 81 , wherein the biorecognition element comprises at least one of a polymer selected from the group consisting of: aniline, pyrrole, acetylene, phenylene, phenylene vinylene, phenylene diamine, thiophene, 3,4-ethylenedioxythiophene, and aminophenylboronic acid.
87. The method of claim 81 , wherein the working electrode is annular and is on only a segment of a surface of the first solid microneedle.
88. The method of claim 81 , wherein a portion of the first body portion, a portion of the second body portion, and a portion of the third body portion is surrounded by an insulative barrier.