Cell culture control device, cell culture device provided with same, cell culture control method, and cell culture control program
A PC comprises a measurement unit, a consumption rate calculation unit, a culture medium replacement detection unit, and a next step transition determination unit. The measurement unit acquires the amount of consumed glucose from the result of continuously measuring the concentration of glucose contained in a culture medium (X) of a culture vessel. The consumption rate calculation unit calculates the consumption rate at which glucose is consumed by differentiating the amount of glucose consumed. The culture medium replacement detection unit detects that the culture medium (X) in the culture vessel has been replaced or added. When the culture medium (X) is replaced or added, the next step transition determination unit determines whether or not to proceed to the next step according to whether or not the consumption rate after the replacement or addition of the culture medium (X) calculated by the consumption rate calculation unit satisfies a specific condition.
1 . A cell culture control device, comprising:
a consumption amount information acquisition unit configured to acquire an amount of consumed nutrients from a result of continuously measuring a concentration of nutrients contained in a culture medium contained in a culture vessel;
a consumption rate calculation unit configured to differentiate the amount of consumed nutrients acquired by the consumption amount information acquisition unit, and calculates the consumption rate at which the nutrients are consumed;
a culture medium replacement detection unit configured to detect that the culture medium contained in the culture vessel has been replaced or added; and
a next step transition determination unit configured to determine whether or not to proceed to a next step according to whether or not the consumption rate after replacement or addition of the culture medium calculated by the consumption rate calculation unit satisfies a specific condition when the culture medium replacement detection unit detects that the culture medium has been replaced or added.
2 . The cell culture control device according to claim 1 ,
wherein the next step transition determination unit determines to proceed to the next step when a difference in the consumption rate before and after replacing or adding the culture medium is equal to or greater than a specific value.
3 . The cell culture control device according to claim 1 ,
wherein the next step transition determination unit determines to proceed to the next step when the consumption rate becomes lower than immediately after replacing or adding the culture medium within a specific length of time.
4 . The cell culture control device according to claim 1 ,
further comprising a consumption acceleration calculation unit configured to differentiate the consumption rate calculated by the consumption rate calculation unit to calculate a consumption acceleration at which the nutrients are consumed,
wherein the next step transition determination unit determines to proceed to the next step when the consumption acceleration calculated by the consumption acceleration calculation unit becomes negative within a specific length of time.
5 . The cell culture control device according to claim 2 ,
wherein the next step transition determination unit determines to proceed to the next step when the consumption rate after replacement or addition of the culture medium is equal to or greater than a specific value.
6 . The cell culture control device according to claim 1 ,
further comprising a control unit configured to perform replacement or addition of the culture medium by controlling an introduction pump that supplies the culture medium to the culture vessel, and a discharge pump that discharges the culture medium from the culture vessel.
7 . The cell culture control device according to claim 6 ,
wherein the culture medium replacement detection unit detects that the culture medium has been replaced or added by detecting that the introduction pump and the discharge pump that have been driven to replace or add the culture medium contained in the culture vessel have stopped.
8 . The cell culture control device according to claim 6 ,
wherein the control unit performs replacement or addition of the culture medium once a specific length of time has elapsed.
9 . The cell culture control device according to claim 1 ,
wherein the culture medium replacement detection unit detects that the culture medium has been replaced or added when there is an input to restart a measurement after the measurement of the nutrients has been paused.
10 . The cell culture control device according to claim 1 ,
further comprising a display unit configured to display a message prompting a user to proceed to the next step when the next step transition determination unit determines to proceed to the next step.
11 . The cell culture control device according to claim 1 ,
further comprising a communication unit configured to transmit a notification prompting a user to proceed to the next step when the next step transition determination unit determines to proceed to the next step.
12 . The cell culture control device according to claim 1 ,
further comprising a measurement unit that is connected to a sensor immersed in the culture medium contained in the culture vessel, and is configured to continuously measure the concentration of the nutrients.
13 . The cell culture control device according to claim 1 ,
wherein, when the culture medium replacement detection unit detects that the culture medium contained in the culture vessel has been replaced,
the consumption amount information acquisition unit calculates the amount of consumed nutrients on the basis of the concentration of the nutrients contained in the culture medium.
14 . The cell culture control device according to claim 1 ,
wherein when the culture medium replacement detection unit detects that the culture medium contained in the culture vessel has been added,
the consumption amount information acquisition unit calculates the amount of consumed nutrients on the basis of the concentration of the nutrients contained in the culture medium and a volume of the culture medium.
15 . A cell culture device, comprising:
the cell culture control device according to claim 1 ;
an introduction pump that is controlled by the cell culture control device and supplies the culture medium to the culture vessel; and
a discharge pump that is controlled by the cell culture control device and discharges the culture medium from the culture vessel.
16 . A cell culture device, comprising:
the cell culture control device according to claim 14 ; and
an introduction pump that is controlled by the cell culture control device and supplies the culture medium to the culture vessel.
17 . A cell culture control method, comprising:
acquiring the amount of consumed nutrients from the result of continuously measuring the concentration of the nutrients contained in the culture medium contained in the culture vessel by a consumption amount information acquisition unit;
calculating the consumption rate at which the nutrients are consumed by differentiating the amount of consumed nutrients acquired by the consumption amount information acquisition unit, by a consumption rate calculation unit;
detecting that the culture medium contained in the culture vessel has been replaced or added by a culture medium replacement detection unit; and
determining whether or not to proceed to the next step according to whether or not the consumption rate after replacement or addition of the culture medium calculated in the consumption rate calculation unit satisfies a specific condition by a next step transition determination unit, when it has been detected in the culture medium replacement detection unit that the culture medium has been replaced or added.
18 . A cell culture control program that causes a computer to execute a cell culture control method comprising:
acquiring the amount of consumed nutrients from the result of continuously measuring the concentration of the nutrients contained in the culture medium contained in the culture vessel by a consumption amount information acquisition unit;
calculating the consumption rate at which the nutrients are consumed by differentiating the amount of consumed nutrients acquired by the consumption amount information acquisition unit, by a consumption rate calculation unit;
detecting that the culture medium contained in the culture vessel has been replaced or added by a culture medium replacement detection unit; and
determining whether or not to proceed to the next step according to whether or not the consumption rate after replacement or addition of the culture medium calculated in the consumption rate calculation unit satisfies a specific condition by a next step transition determination unit, when it has been detected in the culture medium replacement detection unit that the culture medium has been replaced or added.