Apparatus and method for sorting microfluidic particles
A single junction sorter for a microfluidic particle sorter, the single-junction sorter comprising: an input channel, configured to receive a fluid containing particles; an output sort channel and an output waste channel, each connected to the input channel for receiving the fluid therefrom; a bubble generator, operable to selectively displace the fluid around a particle to be sorted and thereby to create a transient flow of the fluid in the input channel; and a vortex element, configured to cause a vortex in the transient flow in order to direct the particle to be sorted into the output sort channel.
1. A method for sorting particles in a particle sorter, the method comprising:
receiving, at an input channel, an input particle suspension comprising a fluid and a plurality of particles;
aligning the plurality of particles in a plurality of streamlines of the fluid;
evaluating the plurality of particles at a predetermined location and assigning, each of the plurality of particles, to one of a plurality of sort designations or a reject designation;
responsive to assigning a particle to a sort designation of the plurality of sort designations, causing a creation of a transient flow in the fluid,
wherein the transient flow is created at a position downstream of the predetermined location;
responsive to causing the creation of the transient flow, causing formation of a sorting vortex in the fluid; and
directing particles having the sort designation, via the sorting vortex, to an output channel of a plurality of output channels based on the assigned sort designation.
2. The method of claim 1 , wherein the plurality of output channels comprises a first sort channel, a second sort channel, and a central reject channel located between the first sort channel and the second sort channel.
3. The method of claim 2 , wherein the central reject channel is fluidly connected to a reject manifold configured to receive the plurality of particles having the reject designation.
4. The method of claim 1 , wherein the plurality of output channels comprises a first pair of sort channels, a second pair of sort channels, and a central reject channel located between the first pair of sort channels and the second pair of sort channels.
5. The method of claim 1 , wherein aligning the plurality of particles is performed using an inertial focuser comprising a plurality of circular arcs of alternating direction.
6. The method of claim 5 , wherein each circular arc from the plurality of circular arcs has an approximate radius of 210 μm and an approximate arc angle of 90°.
7. The method of claim 1 , wherein the plurality of particles in the plurality of streamlines is aligned via at least one of inertial focusing, hydrodynamic focusing, acoustic focusing, or dielectrophoretic focusing.
8. The method of claim 1 , wherein causing formation of the sorting vortex comprises repeatedly applying an actuation pulse at a predetermined time delay that is determined based on the position of the particle.