Method and device for purification of blood from circulating cell free DNA
The invention provides apheresis devices and their use for removal of substantially all types of cell free DNA (cfDNA) in patients' blood, including nucleosome-bound cfDNA, exosome-bound cfDNA and unbound cfDNA (including double stranded DNA (dsDNA), single stranded DNA (ssDNA) and oligonucleotides), to limit the negative effects of the circulating cfDNA and to treat various diseases.
1. A device comprising one or more affinity matrices, wherein said one or more affinity matrices are capable of capturing nucleosome-bound cell free DNA (cfDNA), exosome-bound cfDNA, and unbound cfDNA from a bodily fluid of a subject, wherein at least one of said one or more affinity matrices comprises a linker histone.
2. The device of claim 1 , wherein the unbound cfDNA comprises dsDNA, ssDNA and oligonucleotides.
3. The device of claim 1 , wherein linker histone is human H1.3 linker histone.