Media for stem cell proliferation and induction
The present application discloses a cell culture media for growth, maintenance and induction of reversion to a less mature state of a cell comprising a MUC1* activating ligand.
1. A cell culture media for growth, maintenance, and induction of reversion to a less mature state of a cell comprising a recombinant NME7 protein that comprises a nucleotide diphosphate kinase (NDPK) A domain and an NDPK B domain and does not comprise a targeting sequence.
2. The cell culture media according to claim 1 , wherein the cell is a stem or progenitor cell.
3. The cell culture media according to claim 1 , wherein the cell is a mature, somatic, or progenitor cell.
4. The cell culture media according to claim 1 , wherein the cell is human.
5. The cell culture media according to claim 1 , wherein the cell culture media does not comprise basic fibroblast growth factor (bFGF), transforming growth factor beta (TGF-beta), or both.
6. The cell culture media according to claim 1 , wherein the cell culture media is free of serum.
7. The cell culture media according to claim 1 , wherein the cell culture media further comprises insulin, selenium, transferrin, L-ascorbic acid, or one or more non-essential amino acids.
8. The cell culture media according to claim 1 , wherein the recombinant NME7 is characterized by a molecular weight of about 25-33 kilodaltons (kDa).
9. The cell culture media according to claim 1 , further comprising an inhibitor of a rho associated kinase.
10. The cell culture media according to claim 1 , further comprising an activator of one or more signaling proteins in the PI3K or RAC pathway and in the absence of a rho kinase inhibitor.
11. The cell culture media according to claim 1 , further comprising nucleic acids that suppress expression of NME1 or NME2.
12. The cell culture media according to claim 1 , further comprising other growth factors.
13. The cell culture media according to claim 12 , wherein the other growth factors are FGF-2 and/or TGF-beta.
14. The cell culture media according to claim 1 , wherein the recombinant NME7 is the only growth factor in the cell culture media.
15. The cell culture media according to claim 1 , wherein the recombinant NME7 is characterized by a molecular weight of about 30 kilodaltons (kDa).
16. The cell culture media according to claim 1 , wherein the recombinant NME7 is characterized by a molecular weight of about 31 kilodaltons (kDa).
17. The cell culture media according to claim 1 , wherein the recombinant NME7 is characterized by a molecular weight of about 33 kilodaltons (kDa).
18. The cell culture media according to claim 1 , wherein the recombinant NME7 comprises an alternative splice isoform having an amino acid sequence of SEQ ID NO: 39, 41, 59, or 61.
19. The cell culture media according to claim 1 , wherein the cell culture media further comprises an activator of one or more signaling proteins in the PBK or RAC pathway.