Tissue chamber
The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.
1. A container for holding a tissue sample, the container comprising a surface comprising a frangible area and a plurality of features configured to support a tissue sample and permit fluid flow between the tissue sample and the surface.
2. The container of claim 1 , wherein the plurality of features comprise a material having a refractive index approximately equal to a refractive index of a fluid to be used in processing the tissue sample.
3. The container of claim 2 , wherein the refractive index of the fluid to be used in processing the tissue sample is approximately equal to a refractive index of a structure of the tissue sample to be analyzed.
4. The container of claim 3 , wherein the plurality of features comprise a material having a refractive index of about 1.5 to about 1.7.
5. The container of claim 4 , wherein the plurality of features comprise a material having a refractive index of between about 1.53 and about 1.60.
6. The container of claim 1 , wherein the plurality of features comprise a material that dissolves in the presence of an organic solvent.
7. The container of claim 6 , wherein the organic solvent is a clearing solution.
8. The container of claim 1 , comprising a porous compressible material configured to contact the tissue sample on a side of the tissue sample opposite the surface.
9. The container of claim 8 , wherein the porous compressible material has a refractive index that is approximately equal to a refractive index of the tissue sample to be analyzed.
10. The container of claim 1 , wherein at least a portion of the surface comprises a refractive index approximately equal to a refractive index of a fluid to be used in processing the tissue sample.
11. The container of claim 10 , wherein the refractive index of the fluid to be used in processing the tissue sample is approximately equal to the refractive index of a structure of the tissue sample to be analyzed.
12. The container of claim 10 , wherein the at least a portion of the surface comprises a refractive index of about 1.5 to about 1.7.
13. The container of claim 1 , wherein the frangible area defines a perimeter.
14. The container of claim 13 , wherein the frangible area comprises an area of thinned material relative to an area inside the perimeter.
15. The container of claim 1 , further comprising one or more wax-retention members extending from the surface on a same side as the plurality of features.
16. The container of claim 1 , further comprising one or more locating members extending from a side of the surface opposite the plurality of features.
17. The container of claim 1 , wherein the surface at least partially defines a cavity for receiving the tissue sample.
18. The container of claim 17 , comprising one or more fluid ports in fluid communication with the cavity for receiving the tissue sample and a space outside the cavity.
19. The container of claim 17 , wherein the cavity for receiving tissue samples comprises a frangible area.