Cyclic peptide combinatorial libraries
Novel cyclic peptides, cyclic peptide conjugates and compositions containing them for treating neurological diseases in a subject include an Odorranalectin (OL) sequence or modified OL sequence as a scaffold and a biologically active peptide or protein and/or therapeutic agent conjugated thereto. Methods of treatment of neurological diseases are based on intranasal delivery of a cyclic peptide or cyclic peptide conjugate as described herein. Combinatorial libraries that include a plurality of cyclic peptides have also been developed and can be used to screen for a ligand(s) for a receptor of interest.
1. A combinatorial library comprising a plurality of distinct cyclic peptides of
Formula II:
Tyr-Ala-Ser-Pro-Lys-cyclo-[Cys-Phe-Arg-XXXXX-Leu-Ala-Cys]-Thr (SEQ ID NO: 18), wherein each X is an amino acid selected from the group consisting of an D-amino acid, an L-amino acid, a non-natural synthetic amino acid, a naturally occurring amino acid, and a mixture thereof, said combinatorial library being free from amino acid sequence SEQ ID NO: 1, and
wherein each cyclic peptide of at least a portion of the cyclic peptides has an affinity for at least one opioid receptor and is an agonist or antagonist of the at least one opioid receptor.
2. The combinatorial library of claim 1 , wherein the at least one opioid receptor is selected from the group consisting of μ-opioid receptor (MOR), δ-opioid receptor (DOR), and κ-opioid receptor (KOR).
3. The combinatorial library of claim 1 , wherein the combinatorial library is a positional-scanning synthetic combinatorial library.