IP Library › Patent Application 18171849
Patent Application
App. No. 18/171,849

Bacillus Isolates and Uses Thereof

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Patent No.
US None
App. No.
18/171,849
Abstract

The present disclosure provides isolated Bacillus strains, as well as compositions comprising one or more of the isolated strains and methods of using the isolated strains and compositions to enhance crop growth/yield.

Claims (24)

1 . A method, comprising introducing the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67352 ( B. megaterium NRRL B-67352), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67357 ( B. megaterium NRRL B-67357), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67521 ( B. megaterium NRRL B-67521), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67522 ( B. megaterium NRRL B-67522), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67533 ( B. megaterium NRRL B-67533), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67534 ( B. megaterium NRRL B-67534), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67525 ( B. megaterium NRRL B-67525), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67526 ( B. megaterium NRRL B-67526), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67527 ( B. megaterium NRRL B-67527), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67528 ( B. megaterium NRRL B-67528), the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67529 ( B. megaterium NRRL B-67529) and/or the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67530 ( B. megaterium NRRL B-67530) into a plant growth medium in an amount/concentration sufficient to enhance

(a) nutrient uptake and/or accumulation,

(b) growth, and/or

(c) yield

of a plant grown in said plant growth medium, as compared to a corresponding control plant grown in a corresponding control plant growth medium to which said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 was/were not introduced.

2 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance the calcium, iron, magnesium, manganese, nitrogen, phosphorous, potassium and/or sulfur uptake of said plant.

3 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance the calcium, iron, magnesium, manganese, nitrogen, phosphorous, potassium and/or sulfur accumulation of said plant.

4 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance the calcium uptake and/or accumulation of said plant.

5 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance iron uptake and/or accumulation of said plant.

6 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance magnesium uptake and/or accumulation of said plant.

7 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance manganese uptake and/or accumulation of said plant.

8 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance nitrogen uptake and/or accumulation of said plant.

9 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance phosphorous uptake and/or accumulation of said plant.

10 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance potassium uptake and/or accumulation of said plant.

11 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount sufficient to enhance sulfur uptake and/or accumulation of said plant.

12 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount sufficient to enhance the growth of said plant

13 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium in an amount/concentration sufficient to enhance the yield of said plant.

14 . The method of claim 1 , wherein said plant is a cereal plant.

15 . The method of claim 1 , wherein said plant is a corn plant.

16 . The method of claim 1 , wherein said plant is a wheat plant.

17 . The method of claim 1 , wherein said plant is a leguminous plant.

18 . The method of claim 1 , wherein said plant is a soybean plant.

19 . The method of claim 1 , wherein said plant is a grass plant.

20 . The method of claim 1 , wherein said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530 is/are introduced into said plant growth medium as part of a fertilizer comprising one or more plant nutrients, and wherein said corresponding control plant growth medium was treated with a corresponding control fertilizer devoid of said B. megaterium NRRL B-67352, B. megaterium NRRL B-67357, B. megaterium NRRL B-67521, B. megaterium NRRL B-67522, B. megaterium NRRL B-67533, B. megaterium NRRL B-67534, B. megaterium NRRL B-67525, B. megaterium NRRL B-67526, B. megaterium NRRL B-67527, B. megaterium NRRL B-67528, B. megaterium NRRL B-67529 and/or B. megaterium NRRL B-67530.

Assignments (1)
CHANGE OF NAME AND ADDRESS Recorded Oct 7, 2024
From: NOVOZYMES BIOAG A/S
To: NOVONESIS PLANT BIOSOLUTIONS A/S
Reel/Frame 070166/0088 →