Polymorphs of a dihydroorotate dehydrogenase (DHOD) inhibitor
The present disclosure provides polymorphs of 3-(2,3,5,6-tetrafluoro-3′-trifluoromethoxy-biphenyl-4-ylcarbamoyl)-thiophene-2-carboxylic acid, and methods of making such polymorphs. The present disclosure further provides pharmaceutical compositions and kits comprising polymorphs of the invention, and methods of treating, preventing, or ameliorating a disease or condition comprising administering a polymorph of the invention.
1. A polymorph of 3-(2,3,5,6-tetrafluoro-3′-trifluoromethoxy-biphenyl-4-ylcarbamoyl)-thiophene-2-carboxylic acid as shown in Formula I:
wherein the polymorph is
Form A comprising an X-ray powder diffraction (XRPD) comprising three or more 2θ values selected from 14.8±0.2, 15.6±0.2, 16.5±0.2, 19.6±0.2, 20.8±0.2, 25.6±0.2 and 26.1±0.2; or
Form B comprising an X-ray powder diffraction (XRPD) comprising three or more 2θ values selected from 14.9±0.2, 17.6±0.2, 19.3±0.2, 20.9±0.2, 24.0±0.2, 24.9±0.2 and 25.4±0.2.
2. The polymorph of claim 1 , wherein the polymorph is Form A.
3. The polymorph of claim 2 , wherein the polymorph comprises four or more 2θ values selected from 14.8±0.2, 15.6±0.2, 16.5±0.2, 19.6±0.2, 20.8±0.2, 25.6±0.2 and 26.1±0.2.
4. The polymorph of claim 2 , wherein Form A comprises the XRPD pattern shown in FIG. 2 A .
5. The polymorph of claim 2 further comprising one or more characteristics selected from:
a. a DSC spectrum (5° C./min) comprising an endotherm onset at 178.3±1.0° C., and a second endotherm onset at 227.3=1.0° C.;
b. a DSC spectrum (10° C./min) comprising an endotherm onset at 177.6±1.0° C., and an endotherm onset at 227.9±1.0° C.;
c. a DSC spectrum (20° C./min) comprising an endotherm onset at 178.5±1.0° C., and an endotherm onset at 222.5±1.0° C. and 224.3±1.0° C., followed by an endotherm onset at 227.8±1.0° C.;
d. a DSC spectrum (50° C./min) comprising double endotherm onsets at 88.0±1.0° C. and 92.6±1.0° C., a second endotherm onset at 180.5±1.0° C., a sharp endo/exo onset at 224.7±1.0° C., and an endotherm onset at 229.5±1.0° C.;
e. a melt-quench-cool (MDSC) comprising an endotherm onset at 177.7±1.0° C., an endotherm onset at 227.8±1.0° C.; or
f. a VT-XRPD pattern with observable changes at 200° C.
6. The polymorph of claim 1 , wherein the polymorph is Form B.
7. The polymorph of claim 6 , wherein the polymorph comprises four or more 2θ values selected from 14.8±0.2, 17.6±0.2, 19.3±0.2, 20.9±0.2, 24.0±0.2, 24.9±0.2 and 25.4±0.2.
8. The polymorph of claim 6 , wherein Form B comprises the XRPD pattern shown in FIG. 2 B .
9. A pharmaceutical composition comprising the polymorph Form A or Form B of claim 1 .
10. A kit comprising the polymorph Form A or Form B of claim 1 .
11. A method of preparing polymorph Form A of 3-(2,3,5,6-tetrafluoro-3′-trifluoromethoxy-biphenyl-4-ylcarbamoyl)-thiophene-2-carboxylic acid, the method comprising:
a) obtaining a sample comprising 3-(2,3,5,6-tetrafluoro-3′-trifluoromethoxy-biphenyl-4-ylcarbamoyl)-thiophene-2-carboxylic acid;
b) dissolving the sample of (a) in a solvent comprising C 1 -C 5 alcohol, C 1 -C 5 ketone, or C 1 -C 5 acetate solvent at 20° C. to 60° C. to form a solution; and
c) removing the solvent from the solution to obtain the polymorph Form A of claim 1 .
12. The method of claim 11 , wherein the C 1 -C 5 alcohol is selected from methanol, ethanol, 1-propanol, isopropanol, 1-butanol, 2-butanol, 1-pentanol, 2-pentanol, or isopentanol, the C 1 -C 5 ketone is selected from methyl ethyl ketone (MEK), methylisobutyl ketone (MIBK), or acetone, and the C 1 -C 5 acetate is selected from methyl acetate, ethyl acetate, propyl acetate, butyl acetate, or pentyl acetate.
13. The method of claim 11 , wherein the solution is greater than 80% C 1 -C 5 alcohol, C 1 -C 5 ketone, or C 1 -C 5 acetate.
14. The method of claim 11 , wherein the solution comprises 0% to 20% water.
15. The method of claim 11 , wherein after the dissolving in (b), the solution is cooled to an incubation temperature of 1° C. to 10° C. for a period of time.
16. The method of claim 15 , wherein the cooling to the incubation temperature is at a rate of about 0.02° C./min to about 1° C./min until the incubation temperature is reached.
17. The method of claim 15 , wherein the period of time for the incubation temperature is about 1 hour to about 48 hours.
18. The method of claim 11 , wherein the removing of the solvent is performed by evaporation or filtration.
19. The method of claim 18 , wherein the evaporation occurs at about 20° C. to about 35° C.
20. A method of treating or ameliorating a disease or condition in a subject in need thereof comprising administering to the subject a pharmaceutically effective amount of polymorph Form A of claim 1 , wherein the disease or condition is an inflammatory disease or disorder.