Anti-transferrin receptor antibody and uses thereof
Aspects of the disclosure relate to antibodies that bind to transferrin receptor (e.g., transferrin receptor 1) and complexes comprising the antibody covalently linked to a molecular payload. Methods of using the antibodies are also provided.
1. A method of producing an antibody that binds to human transferrin receptor (TfR), wherein the antibody is a Fab and comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 101 and a light chain comprising the amino acid sequence of SEQ ID NO: 90, and wherein the method comprises culturing a cell comprising a nucleic acid sequence encoding the heavy chain of the antibody and a nucleic acid sequence encoding the light chain of the antibody.
2. The method of claim 1 , wherein the nucleic acid sequence encoding the heavy chain and the nucleic acid sequence encoding the light chain are on the same vector.
3. The method of claim 1 , wherein the nucleic acid sequence encoding the heavy chain and the nucleic acid sequence encoding the light chain are on different vectors.
4. The method of claim 1 , wherein the nucleic acid sequence encoding the heavy chain is operably linked to a promoter.
5. The method of claim 1 , wherein the nucleic acid sequence encoding the light chain is operably linked to a promoter.
6. The method of claim 1 , wherein the nucleic acid sequence encoding the heavy chain and the nucleic acid sequence encoding the light chain is operably linked to a promoter.
7. The method of claim 4 , wherein the promoter is an inducible promoter.
8. The method of claim 5 , wherein the promoter is an inducible promoter.
9. The method of claim 6 , wherein the promoter is an inducible promoter.
10. The method of claim 1 , wherein the cell is a CHO cell.
11. The method of claim 10 , wherein the CHO cell is a dhfr-CHO cell.
12. The method of claim 10 , wherein the CHO cell is a CHO-S cell.
13. The method of claim 10 , wherein the CHO cell is a CHO-K1 cell.
14. The method of claim 1 , further comprising isolating the antibody.
15. The method of claim 14 , wherein the antibody is isolated via affinity chromatography.
16. The method of claim 14 , wherein the antibody is isolated by affinity chromatography with a Protein A or Protein G coupled matrix.