NUCLEOSIDES AND NUCLEOTIDES WITH 3' VINYL BLOCKING GROUP
Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with an optionally substituted 3′vinyl blocking group. Also provided herein are methods to prepare such nucleotide and nucleoside molecules. Additionally, the present disclosure provides methods of using such blocked nucleosides and nucleotides in oligonucleotide synthesis and sequencing.
1 . A nucleoside or nucleotide having the structure of Formula (I):
or a salt thereof, wherein
B comprises a nucleobase;
R 1 is H, hydroxy, —OR 5 , halo, or a hydroxy protecting group;
R 2 is H;
R 3 is H, a hydroxy protecting group, or —OR 3 is a monophosphate, diphosphate, triphosphate or phosphorothioate;
each of R 4a , R 4b and R 4c is independently H, halo, C 1 -C 3 alkyl, C 1 -C 3 haloalkyl, C 1 -C 3 hydroxyalkyl, azido, optionally substituted phenyl, optionally substituted 5 to 6 membered heteroaryl, optionally substituted C 3 -C 7 cycloalkyl, or optionally substituted 3 to 7 membered heterocyclyl; and
R 5 is C 1 -C 6 alkyl or C 1 -C 6 haloalkyl, or when R 1 is —OR 5 , R 5 and R 2 together with the atoms to which they are attached form a four to seven membered heterocycle containing one oxygen atom.
2 . The nucleoside or nucleotide of claim 1 , wherein each of R 4a , R 4b and R 4c is H, and the nucleoside or nucleotide has the structure of Formula (Ia):
or a salt thereof.
3 . The nucleoside or nucleotide of claim 1 , wherein at least one of R 4a , R 4b and R 4c is methyl, ethyl, n-propyl, isopropyl, fluoro, chloro, —CHF 2 , —CH 2 F, —CH 2 C 1 , —CHCl 2 , or —CF 3 .
4 . The nucleoside or nucleotide of claim 1 , wherein R 1 is H.
5 . The nucleoside or nucleotide of claim 1 , wherein R 1 is hydroxy, or a hydroxy protecting group selected from the group consisting of
covalently attached to the 2′ oxygen atom.
6 . The nucleoside or nucleotide of claim 1 , wherein R 1 is fluoro.
7 . The nucleoside or nucleotide of claim 1 , wherein R 1 is —OR 5 and R 5 is methyl.
8 . The nucleoside or nucleotide of claim 1 , wherein R 2 is H.
9 . The nucleoside or nucleotide of claim 1 , wherein R 1 is —OR 5 and R 5 and R 2 together with the atoms to which they are attached form a four to seven membered heterocycle having the Formula (Ib):
or a salt thereof.
10 . The nucleoside or nucleotide of claim 9 , having the structure of Formula (Ib′):
or a salt thereof.
11 . The nucleoside or nucleotide of claim 10 , wherein each of R 4a , R 4b and R 4c is H, and the nucleoside or nucleotide has the structure of Formula (Ic):
or a salt thereof.
12 . The nucleoside or nucleotide of claim 1 , wherein —OR 3 is a monophosphate, diphosphate or triphosphate.
13 . The nucleoside of claim 1 , wherein the nucleobase is:
wherein R x is H or an amino protecting group, or the hydrogen in —NHR x is absent and R x is a divalent amino protecting group.
14 . The nucleoside or nucleotide of claim 13 , wherein R x is H, —C(═O)C 1-6 alkyl or —C(═O)-phenyl.
15 . The nucleoside or nucleotide of claim 1 , wherein B comprises a nucleobase covalently bounded to a detectable label, optionally through a linker.
16 . The nucleoside or nucleotide of claim 15 , B is
17 . The nucleoside or nucleotide of claim 1 , wherein the nucleoside or nucleotide is nucleotide, and —OR 3 is triphosphate.
18 . A method of controlled synthesis of an oligonucleotide or polynucleotide, comprising:
contacting a nucleotide of claim 17 with the oligonucleotide or polynucleotide in the presence of a polymerase; and
incorporating the nucleotide to the 3′ end of the oligonucleotide or polynucleotide.
19 . The method of claim 18 , further comprising:
removing the 3′vinyl blocking group of the incorporated nucleotide to generate a 3′ hydroxy group on the incorporated nucleotide, and
incorporating a second nucleotide.
20 . The method of claim 18 , wherein the polymerase is a template independent polymerase.
21 . (canceled)
22 . The method of claim 20 , wherein the template independent polymerase is terminal deoxynucleotidyl transferase (TdT), PolyA polymerase, or CCA-adding RNA polymerase.
23 . The method of claim 19 , wherein the removing of the 3′vinyl blocking group of the incorporated nucleotide is achieved by a tetrazine reagent.
24 . The method of claim 23 , wherein the tetrazine reagent is selected from the group consisting of
and optionally substituted variants thereof.
25 . (canceled)
26 . A method of synthesizing a nucleotide of Formula (Id):
or a salt thereof, comprising:
reacting a nucleoside of Formula (Ie):
with
in the presence of a peroxide to form a first intermediate of Formula (Ie-1):
reacting the first intermediate with 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) to form a second intermediate of Formula (Ie-2):
reacting the second intermediate with 2-methyl-1H-imidazole to form the third intermediate of Formula (Ie-3):
and
reacting the third intermediate with pyrophosphate H 2 P 2 O 7 to generate the compound of Formula (Id);
wherein B comprises a nucleobase;
R 1 is H, hydroxy, —OR 5 , halo, or a hydroxy protecting group;
R 2 is H;
each of R 4a , R 4b and R 4c , is independently H, halo, C 1 -C 3 alkyl, C 1 -C 3 haloalkyl, C 1 -C 3 hydroxyalkyl, azido, optionally substituted phenyl, optionally substituted 4 to 6 membered heteroaryl, optionally substituted C 3 -C 7 cycloalkyl, or optionally substituted 3 to 7 membered heterocyclyl; and
R 5 is C 1 -C 6 alkyl or C 1 -C 6 haloalkyl, or alternatively, when R 1 is —OR 5 , R 5 and R 2 together with the atoms to which they are attached form a four to seven membered heterocycle containing one oxygen atom.
27 . (canceled)
28 . (canceled)