IP Library › Granted Patent US 12,436,317
Granted Patent B2
US 12,436,317 · App. 18/223,289 · Granted Oct 7, 2025

High throughput screening system for engineered cardiac tissues

Inventors: Thomas Bifano (Mansfield, MA); Jerome Mertz (Newton, MA); Marshall Ma (Watertown, MA); Devin Beaulieu (Brookline, MA)
Assignees: Trustees of Boston University; Massachusetts Institute of Technology
G02B3/0037G02B13/22G02B19/0047G02B21/0076G02B21/06
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Quick Facts
Patent No.
US 12,436,317
App. No.
18/223,289
Granted
Oct 7, 2025
Kind
B2
Abstract

An exemplary imaging system is provided. The imaging system includes a light source configured to illuminate a plurality of spatially separated regions of a material structure producing a first illumination. A lens produces an image of the spatially separated regions. A lens array magnifies the spatially separated regions of the image. The lens array produces a mosaic image comprised of magnified subimages of each region spatially separated region. A camera sensor to record the image.

Claims (31)

1. An imaging system comprising:

a light source configured to illuminate directly a plurality of spatially separated regions to generate a first illumination, each of the spatially separated regions including a material surface;

a lens array that includes one or more achromatic doublet lenses for magnifying the spatially separated regions, the lens array directly receiving the first illumination from the illuminating of the spatially separated regions, wherein the lens array generates a second illumination that magnifies the spatially separated regions, and wherein each lens of the lens array is concentrically aligned with a center of each of the spatially separated regions; and

a telecentric lens for directly receiving the second illumination from the lens array, wherein the telecentric lens directs the second illumination to a camera sensor to generate a mosaic image of the magnified spatially separated regions, and wherein the camera sensor records the mosaic image.

2. The imaging system of claim 1 , wherein the light source comprises a white light emitting diode panel.

3. The imaging system of claim 1 , wherein the light source comprises a laser or LED illumination intended to excite fluorescence in the spatially separated regions, and an optical filter is positioned in front of the camera sensor to reject excitation light but pass fluorescently emitted light from the spatially separated regions.

4. The imaging system of claim 1 , wherein the material surface is a surface of an engineered cardio tissue.

5. The imaging system of claim 1 , wherein the material surface is a surface of a biological sample.

6. The imaging system of claim 1 , wherein an optical rail is configured to adjust a position of the telecentric lens.

7. The imaging system of claim 1 , wherein the lens array is disposed between the spatially separated regions and the telecentric lens.

8. The imaging system of claim 1 , wherein the telecentric lens includes at least one of a bi-telecentric lens.

9. The imaging system of claim 1 , wherein modification of an axial placement of the lens array allows variance in magnification of the mosaic image.

10. The imaging system of claim 9 , wherein a variance in magnification of the mosaic images ranges from 3× to 6×.

11. A method for imaging an object, the method comprising:

providing a light source configured to illuminate directly a plurality of spatially separated regions to generate a first illumination, each of the spatially separated regions including a material surface;

magnifying, using a lens array that includes one or more achromatic doublet lenses, the spatially separated regions, the lens array directly receiving the first illumination from the illuminating of the spatially separated regions, wherein the lens array generates a second illumination that magnifies the spatially separated regions, and wherein each lens of the lens array is concentrically aligned with a center of each of the spatially separated regions;

providing a telecentric lens for directly receiving the second illumination from the lens array, wherein the telecentric lens directs the second illumination to a camera sensor to generate a mosaic image of the magnified spatially separated regions; and

recording, using the camera sensor, the mosaic image of the magnified spatially separated regions.

12. The method of claim 11 , wherein the light source comprises a white light emitting diode panel.

13. The method of claim 11 , wherein the light source comprises a laser or LED illumination intended to excite fluorescence in the spatially separated regions, and an optical filter is positioned in front of the camera sensor to reject excitation light but pass fluorescently emitted light from the spatially separated regions.

14. The method of claim 11 , wherein the material surface is a surface of an engineered cardio tissue.

15. The method of claim 11 , wherein the material surface is a surface of a biological sample.

16. The method of claim 11 , wherein an optical rail is configured to adjust a position of the telecentric lens.

17. The method of claim 11 , wherein the lens array is disposed between the spatially separated regions and the telecentric lens.

18. The method of claim 11 , wherein the telecentric lens includes at least one of a bi-telecentric lens.

19. The imaging system of claim 11 , wherein modification of an axial placement of the lens array allows a variance in magnification of the mosaic image.

20. The imaging system of claim 19 , wherein the variance in magnification of the mosaic images ranges from 3× to 6×.

21. An imaging system comprising:

a light source configured to illuminate directly a plurality of spatially separated regions to generate a first illumination, each of the spatially separated regions including a material surface;

a lens array that includes one or more achromatic doublet lenses for magnifying the spatially separated regions, the lens array directly receiving the first illumination from the illuminating of the spatially separated regions, wherein the lens array generates a second illumination that magnifies the spatially separated regions; and

a telecentric lens for directly receiving the second illumination from the lens array, wherein the telecentric lens directs the second illumination to a camera sensor to generate a mosaic image of the magnified spatially separated regions, and wherein each lens of the lens array is concentrically aligned with a center of each of the spatially separated regions, and wherein the camera sensor records the mosaic image, and the mosaic image is used to identify a property of the spatially separated regions.

Assignments (3)
CONFIRMATORY LICENSE Recorded May 12, 2025
From: BOSTON UNIVERSITY CHARLES RIVER CAMPUS
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 071249/0566 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 29, 2023
From: BIFANO, THOMAS; MERTZ, JEROME; MA, MARSHALL
To: TRUSTEES OF BOSTON UNIVERSITY
Reel/Frame 064738/0965 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 29, 2023
From: BEAULIEU, DEVIN
To: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Reel/Frame 064739/0053 →
Continuity (2)
Provisional Application 63390469 · Jul 19, 2022
Related Publication 20240027655A1 · Jan 25, 2024
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