ANTI-SARS-COV-2 ANTIBODIES DERIVED FROM CR3022
This disclosure provides antibodies and that can be administered to an individual that is infected with a virus. Antibodies herein can be capable of treating or curing the virus, and which may provide protection against the virus for up to several weeks. Antibodies herein can be used to diagnose a SARS-CoV-2 infection.
1 . An antibody or an antigen-binding fragment that selectively binds to a severe acute respiratory syndrome coronavirus 2 (SARS-Co V-2), that comprises:
(i) a variable heavy chain (VH) complementarity determining region 1 (CDR1) having an amino acid sequence of SEQ ID NO: 62, a VH CDR2 having an amino acid sequence of SEQ ID NO: 77, a VH CDR3 having an amino acid sequence of SEQ ID NO: 32, a VL CDR1 having an amino acid sequence of SEQ ID NO: 115, a VL CDR2 having an amino acid sequence of SEQ ID NO: 136, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 161;
(ii) a VH CDR1 having an amino acid sequence of SEQ ID NO: 44, a VH CDR2 having an amino acid sequence of SEQ ID NO: 71, a VH CDR3 having an amino acid sequence of SEQ ID NO: 15, a VL CDR1 having an amino acid sequence of SEQ ID NO: 10, a VL CDR2 having an amino acid sequence of SEQ ID NO: 11, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 143; or
(iii) a VH CDR1 having an amino acid sequence of SEQ ID NO: 46, a VH CDR2 having an amino acid sequence of SEQ ID NO: 74, a VH CDR3 having an amino acid sequence of SEQ ID NO: 16, a VL CDR1 having an amino acid sequence of SEQ ID NO: 103, a VL CDR2 having an amino acid sequence of SEQ ID NO: 122, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 143.
2 . The antibody or antigen-binding fragment of claim 1 , that comprises a VH CDR1 having an amino acid sequence of SEQ ID NO: 62, a VH CDR2 having an amino acid sequence of SEQ ID NO: 77, a VH CDR3 having an amino acid sequence of SEQ ID NO: 32, a VL CDR1 having an amino acid sequence of SEQ ID NO: 115, a VL CDR2 having an amino acid sequence of SEQ ID NO: 136, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 161.
3 . The antibody or antigen-binding fragment of claim 1 , that comprises a VH CDR1 having an amino acid sequence of SEQ ID NO: 44, a VH CDR2 having an amino acid sequence of SEQ ID NO: 71, a VH CDR3 having an amino acid sequence of SEQ ID NO: 15, a VL CDR1 having an amino acid sequence of SEQ ID NO: 10, a VL CDR2 having an amino acid sequence of SEQ ID NO: 11, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 143.
4 . The antibody or antigen-binding fragment of claim 1 , that comprises a VH CDR1 having an amino acid sequence of SEQ ID NO: 46, a VH CDR2 having an amino acid sequence of SEQ ID NO: 74, a VH CDR3 having an amino acid sequence of SEQ ID NO: 16, a VL CDR1 having an amino acid sequence of SEQ ID NO: 103, a VL CDR2 having an amino acid sequence of SEQ ID NO: 122, and a VL CDR3 having an amino acid sequence of SEQ ID NO: 143.
5 . The antibody or antigen-binding fragment of claim 1 , that comprises a VH having an amino acid sequence of SEQ ID NO: 224 and a VL having an amino acid sequence of SEQ ID NO: 279.
6 . The antibody or antigen-binding fragment of claim 1 , that comprises a VH having an amino acid sequence of SEQ ID NO: 192 and a VL having an amino acid sequence of SEQ ID NO: 248.
7 . The antibody or antigen-binding fragment of claim 1 , that comprises a VH having an amino acid sequence of SEQ ID NO: 195 and a VL having an amino acid sequence of SEQ ID NO: 251.
8 . The antibody or the antigen-binding fragment of claim 1 , that selectively binds to a receptor binding domain (RBD) of SARS-CoV-2.
9 . The antibody or the antigen-binding fragment of claim 1 , wherein the antibody is an IgG, an IgM, an IgE, an IgA, an IgD, or is derived therefrom.
10 . The antibody or the antigen-binding fragment of claim 9 , wherein the antibody is an IgG selected from the group consisting of an IgG1, an IgG2a, an IgG2b, an IgG3, and an IgG4.
11 . The antibody or the antigen-binding fragment of claim 1 , wherein the antibody comprises a monoclonal antibody, a grafted antibody, a chimeric antibody, a human antibody, or a humanized antibody.
12 . The antibody or the antigen-binding fragment of claim 1 , that comprises a binding affinity of less than 50 nM.
13 . The antibody or the antigen-binding fragment of claim 1 , wherein the antigen-binding fragment comprises a Fab, a Fab′, a F(ab′) 2 , a variable fragment (Fv), a triabody, a tetrabody, a minibody, a bispecific F(ab′) 2 , a trispecific F(ab′) 2 , a diabody, a bispecific diabody, a single chain variable fragment (scFv), a scFv-Fc, a Fab-Fc, a VHH, or a bispecific scFv.
14 . The antibody or the antigen-binding fragment of claim 1 , that is isolated, recombinant, or synthetic.
15 . A method of preventing or treating a SARS-CoV-2 viral infection or COVID19 in a subject in need thereof, comprising administering to the subject the antibody or the antigen-binding fragment of claim 1 .
16 . The method of claim 15 , that further comprises administering one or more additional therapies or drugs to the subject.
17 . A method of preventing or treating a SARS-CoV-2 viral infection or COVID19 in a subject in need thereof, comprising administering to the subject one or more polynucleotide sequences that encode the antibody or the antigen-binding fragment of claim 1 .
18 . A method of diagnosing a subject as being infected with a SARS-CoV-2 virus or suspected of being infected with a SARS-CoV-2 virus, the method comprising contacting a sample obtained from the subject with the antibody or the antigen-binding fragment of claim 1 ; detecting the presence or absence of an antibody/SARS-CoV-2 virus complex or an antigen-binding fragment/SARS-CoV-2 virus complex; and diagnosing the subject as being infected with a SARS-CoV-2 virus when the presence of the antibody/SARS-CoV-2 virus complex or the antigen-binding fragment/SARS-CoV-2 virus complex is detected.
19 . The method of claim 18 , wherein the sample comprises a nasal swab, a tissue sample, saliva, or blood.
20 . The method of claim 18 , wherein detecting the presence or absence of the antibody/SARS-CoV-2 virus complex or the antigen-binding fragment/SARS-CoV-2 virus complex comprises an enzyme linked immunosorbent assay (ELISA), an immunospot assay, a lateral flow assay, flow cytometry, immunohistochemistry, or a western blot.
21 . Use of one or more of the antibodies, or antigen-binding fragments thereof, of claim 1 , for the treatment of a SARS-CoV-2 viral infection or COVID19 in a subject in need thereof.
22 . Use of one or more of the antibodies, or antigen-binding fragments thereof, of claim 1 , in the formulation of a medicament for the treatment of a SARS-CoV-2 viral infection or COVID19 in a subject in need thereof.
23 . Use of one or more polynucleotide sequences that encode the antibody, or antigen-binding fragment thereof, of claim 1 , for the treatment of a SARS-CoV-2 viral infection or COVID19 in a subject in need thereof.
24 . Use of one or more polynucleotide sequences that encode the antibody, or antigen-binding fragment thereof, of claim 1 , in the formulation of a medicament for the treatment of a SARS-CoV-2 viral infection or COVID19 in a subject in need thereof.