IP Library Granted Patent US 12,256,759
Granted Patent B2
US 12,256,759 · App. 18/291,870 · Granted Mar 25, 2025

Methods for the reduction of methane production in ruminants

Inventors: Abed Chaudhury (Orange, AU); Karen Paco (St. Paul, MN); Michael Anthony Ayliffe (Canberra, AU); Thomas David Loan (Canberra, AU); Ming Luo (Canberra, AU); Tristan Yang (St. Paul, MN)
Assignee: Loam Bio Pty Ltd
A23K10/16A23K20/189A23K50/10A61K36/062A61K38/44C12N1/145C12N9/0065C12Y111/01018C12R2001/645
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Quick Facts
Patent No.
US 12,256,759
App. No.
18/291,870
Granted
Mar 25, 2025
Kind
B2
Abstract

The present disclosure provides methods for reducing methane emissions from a ruminant comprising administering to the ruminant a composition comprising a fungal strain, wherein the fungal strain comprises a vanadium-dependent haloperoxidase (VHPO) such as Curvularia sp. strain 4388 (NMI Accession No. V22/011149). The disclosure also provides a cell or a biologically pure culture of Curvularia sp. strain 4388 (NMI Accession No. V22/011149) or a mutant thereof having all identifying characteristics of the strain.

Claims (9)

1. A method for reducing methane emissions from a ruminant,

the method comprising administering to the ruminant bromoform produced by a composition comprising:

bromide, and

a vanadium-dependent haloperoxidase (VHPO) comprising SEQ ID NO: 2;

thereby reducing methane emissions in the ruminant relative to a ruminant not administered bromoform produced by the composition.

2. The method of claim 1 , wherein the VHPO is expressed by a fungal strain belonging to the genus Curvularia.

3. The method of claim 1 , wherein the ruminant is a member of the family Bovidae.

4. The method of claim 3 , wherein the ruminant is Bos taurus.

5. The method of claim 2 , wherein the fungal strain is Curvularia sp. strain 4388 (NMI Accession No. V22/011149).

Assignments (7)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 19, 2026
From: LOAM BIO PTY LTD
To: RUMIN8 PTY LTD
Reel/Frame 073509/0081 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 4, 2025
From: ROAM AGRICULTURAL PTY LTD
To: LOAM BIO PTY LTD
Reel/Frame 072783/0134 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 24, 2024
From: LOAM BIO PTY LTD
To: ROAM AGRICULTURAL PTY LTD
Reel/Frame 069010/0027 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 28, 2024
From: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
To: SOILCQUEST PTY LTD
Reel/Frame 068731/0578 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 28, 2024
From: LUO, MING; AYLIFFE, MICHAEL ANTHONY; LOAN, THOMAS DAVID
To: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
Reel/Frame 068731/0575 →
CHANGE OF NAME Recorded Sep 28, 2024
From: SOILCQUEST PTY LTD
To: LOAM BIO PTY LTD
Reel/Frame 068731/0586 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 15, 2024
From: CHAUDHURY, ABED; PACO, KAREN; YANG, TRISTAN
To: LOAM BIO PTY LTD.
Reel/Frame 066475/0001 →
Priority Claims (1)
AU 2021902354 · Jul 30, 2021 · national
Continuity (1)
Related Publication 20240260608A1 · Aug 8, 2024
References Cited (18)
US 9872892B2 · Klausen · 2018 [cited by examiner]
US 20160051636A1 · Klausen · 2016 [cited by examiner]
US 20170196922A1 · Embree et al. · 2017 [cited by applicant]
AU 2021105299A4 · 2021 [cited by applicant]
CN 106978355A · 2017 [cited by applicant]
JP 2005110573A · 2005 [cited by applicant]
WO 9947651 · 1999 [cited by applicant]
WO 2001053494A2 · 2001 [cited by applicant]
WO 2011117552A1 · 2011 [cited by applicant]
WO 2014180953A1 · 2014 [cited by applicant]
WO 2015110663A1 · 2015 [cited by applicant]
WO 2017120495A1 · 2017 [cited by applicant]
WO 2019102279A1 · 2019 [cited by applicant]
WO 2020243792A1 · 2020 [cited by applicant]
WO 2022266723 · 2022 [cited by applicant]
Baumgartner et al. 2021 (Investigating the role of vanadium-dependent haloperoxidase enzymology in microbial secondary metabolism and chemical ecology; mSystems 6(4): 1-5) (Year: 2021). [cited by examiner]
International Search Report and Written Opinion of the International Searching Authority for International Patent Application No. PCT/US2022/039095, 17 pages. [cited by applicant]
Baumgartner, J. T., & McKinnie, S. M. (2021). Investigating the role of vanadium-dependent haloperoxidase enzymology in microbial secondary metabolism and chemical ecology. Msystems, 6(4), 10-1128. [cited by applicant]