Herbicide tolerance genes and methods of use thereof
Polypeptides and recombinant DNA molecules useful for conferring tolerance to AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides are provided in the present invention, as well as herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.
1. A recombinant DNA molecule comprising a nucleic acid sequence encoding a polypeptide having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52, wherein the polypeptide confers herbicide tolerance to a transgenic plant.
2. The recombinant DNA molecule of claim 1 , wherein the nucleic acid sequence is selected from the group consisting of SEQ ID NO:2, 3, 5, 6, 8, 10, 12, 13, 19, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 53-57, and 59.
3. The recombinant DNA molecule of claim 1 , wherein the recombinant DNA molecule is operably linked to a heterologous promoter functional in a plant cell.
4. The recombinant DNA molecule of claim 3 , wherein the recombinant DNA molecule is further operably linked to a DNA molecule encoding a chloroplast transit peptide.
5. A DNA construct comprising a heterologous promoter functional in a plant cell operably linked to the recombinant DNA molecule of claim 1 .
6. The DNA construct of claim 5 , further comprising a DNA molecule encoding a chloroplast transit peptide operably linked to the recombinant DNA molecule.
7. The DNA construct of claim 5 , wherein the nucleic acid sequence encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52.
8. The DNA construct of claim 5 , wherein the DNA construct is present in the genome of a transgenic plant.
9. A plant, seed, plant tissue, plant part, or cell comprising the recombinant DNA molecule of claim 1 .
10. The plant, seed, plant tissue, plant part, or cell of claim 9 , wherein the plant, seed, plant tissue, plant part, or cell comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides.
11. A plant, seed, plant tissue, plant part, or cell comprising the DNA construct of claim 5 .
12. A plant, seed, plant tissue, plant part, or cell comprising the polypeptide encoded by the recombinant DNA molecule of claim 1 .
13. A polypeptide having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52, wherein the polypeptide confers herbicide tolerance to a transgenic plant.
14. The polypeptide of claim 13 , wherein the polypeptide has oxygenase activity against at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides.
15. A method for conferring herbicide tolerance to a plant, seed, cell, or plant part comprising expressing in said plant, seed, cell, or plant part the polypeptide of claim 13 .
16. The method of claim 15 , wherein said plant, seed, cell, or plant part comprises a DNA construct comprising a heterologous promoter functional in a plant cell operably linked to a recombinant DNA molecule comprising a nucleic acid sequence encoding a polypeptide having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52.
17. The method of claim 15 , wherein the plant, seed, cell, or plant part comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides.
18. A method for producing an herbicide tolerant transgenic plant comprising transforming a plant cell or tissue with a recombinant DNA molecule comprising a nucleic acid sequence selected from the group consisting of:
a) a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52;
b) a nucleic acid sequence selected from the group consisting of SEQ ID NO:2, 3, 5, 6, 8, 10, 12, 13, 19, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 53-57, and 59; and
c) a nucleic acid sequence encoding a polypeptide having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52, and capable of conferring herbicide tolerance to a transgenic plant;
and regenerating an herbicide tolerant transgenic plant from the transformed plant cell or tissue.
19. The method of claim 18 , wherein said herbicide tolerant transgenic plant comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides.
20. A method for controlling weeds in a plant growth area, comprising contacting a plant growth area comprising a plant or seed comprising a recombinant DNA molecule comprising a nucleic acid sequence encoding a protein having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52 with at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides, wherein the plant or seed is tolerant to the at least one herbicide.
21. The recombinant DNA molecule of claim 1 , wherein the polypeptide has at least 91% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52.
22. The plant, seed, plant tissue, plant part, or cell of claim 9 , wherein the polypeptide has at least 91% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52.
23. The method of claim 15 , wherein the polypeptide has at least 91% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52.