Gel electrophoresis diagnostic kit and methods of using the same

1. A base for reading a biomarker detector cartridge, wherein the biomarker detector cartridge comprises:

(a) a cartridge body;

(b) a fluid collector;

(c) an electrophoretic cell; and

(d) a nanoswitch;

the base comprising:

a biomarker detector cartridge receiver;

electrical contacts proximate to the cartridge receiver configured to communicate with the electrophoretic cell of the biomarker detector cartridge; and

an electrophoretic cell reader proximate to the cartridge receiver.

2. The base of claim 1 , wherein the electrophoresis cell reader comprises a photodetector.

3. The base of claim 2 , wherein the photodetector comprises one or more of a photomultiplier tube detector and a photodiode detector.

4. The base of claim 3 , wherein the photodiode detector comprises one or more of a PN based detector, a PIN based detector, an APD based detector, a CMOS based detector, and a CCD based detector.

5. The base of claim 1 , wherein the electrophoresis cell reader comprises a light source.

6. The base of claim 1 , wherein the electrophoresis cell reader comprises a light filter.

7. The base of claim 1 , wherein the base comprises a transmitter in communication with the electrophoresis cell reader.

8. The base of claim 1 , wherein the base further comprises one or more of:

a controller;

a transmitter; and

a power source.

9. The base of claim 8 , wherein the controller comprises a microprocessor, a central processing unit (CPU), and/or an on/off switch.

10. A method for detecting a biomarker in a fluid, the method comprising the steps of:

collecting the fluid comprising the biomarker with a fluid collector of a biomarker detector cartridge, wherein the biomarker detector cartridge comprises:

(a) a cartridge body;

(b) the fluid collector;

(c) an electrophoretic cell; and

(d) a nanoswitch;

contacting the fluid with the nanoswitch;

transferring the fluid to the electrophoretic cell;

connecting the biomarker detector cartridge to a base, wherein the base comprises a biomarker detector cartridge receiver, electrical contacts proximate to the cartridge receiver configured to communicate with the electrophoretic cell of the biomarker detector cartridge, and an electrophoretic cell reader proximate to the cartridge receiver;

applying a voltage across a separation medium at the electrophoretic cell; and

reading the separation medium with the electrophoresis cell reader at the base.

11. The method of claim 10 , wherein the step of contacting the fluid with the nanoswitch source comprises delivering a plurality of DNA nanoswitches to the fluid that bind the biomarker.

12. The method of claim 10 , wherein the fluid comprises a bodily fluid.

13. The method of claim 12 , wherein the bodily fluid comprises whole blood, serum, urine, saliva, swabbed samples, mucus, semen, cerebrospinal fluid (CSF), swabbed samples, sputum, menstrual blood, menstrual fluid, or vaginal mucus.

14. The method of claim 10 , wherein the biomarker comprises a biomarker for a biological event.

15. The method of claim 14 , wherein the biomarker for the biological event comprises one or more of a disease biomarker, an inflammation biomarker, a reproduction biomarker, and an aging biomarker.

16. The method of claim 15 , wherein the reproduction biomarker comprises one or more of an ovulation biomarker, a fertilization biomarker, an implantation biomarker, and an embryo development biomarker.

17. The method of claim 10 , wherein the biomarker comprises luteinizing hormone (LH), chorionic gonadotropin (β-hCG), ER/PR, HER-2/neu, EGFR, KRAS, UGT1A1, c-KIT, CD20, CD30, FIP1L1-PDGRFalpha, PDGFR, PML/RARalpha, TPMT, ALK, BRAF, influenza A/B/C proteins, HSF-½ proteins, troponin, CRP, and/or ha1c.

18. The method of claim 10 , wherein collecting the fluid comprising the biomarker includes contacting the fluid with the nanoswitch.

19. The method of claim 10 , wherein reading the separation medium with the electrophoresis cell reader comprises:

directing light from a light source through the electrophoretic cell and separation medium;

filtering light from a light source that enters and/or exits the electrophoretic cell and separation medium; and/or

receiving light that exits the electrophoretic cell and separation medium at a photodetector.

20. The method of claim 10 , wherein the reading comprises providing a concentration of the biomarker.