ACTIVATION OF IMMUNE CELLS

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Patent No.: US None
App. No.: 18/334,098

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Abstract

Bone marrow containers are described herein. Separation systems for separating activated immune cells from other components are described herein. Methods of activating an immune cell are described herein.

Claims (17)

1 . A bone marrow container comprising:

a composition comprising:

a non-ionic hydrophilic branched polysaccharide at a density, wherein, if the composition is contacted with whole blood and centrifuged, said density is sufficient to permit fluid movement of whole blood through the non-ionic hydrophilic branched polysaccharide without separation of erythrocytes,

the non-ionic hydrophilic branched polysaccharide having a molecular weight greater than 20 kDa; and

a TLR2 ligand,

wherein the composition is contained within the container, and wherein the container is sterile.

2 . The bone marrow container of claim 1 , wherein the non-ionic hydrophilic branched polysaccharide has a molecular weight of at least 70 kDa.

3 . The bone marrow container of claim 1 , wherein the non-ionic hydrophilic branched polysaccharide comprises poly(sucrose-co-epichlorhydrin).

4 . The bone marrow container of any one of claim 1 , wherein the non-ionic hydrophilic branched polysaccharide has a density less than 1 gram per liter, for example less than 1 g/l, 0.99 g/l, 0.9 g/l, 0.8 g/l, 0.7 g/l, 0.6 g/7, 0.5 g/l, 0.4 g/l, 0.3 g/l, 0.2 g/l, 0.1 g/l, 0.05 g/l, 10 −2 g/l, 2×10 −3 g/l, 10 −3 g/l, 10 −5 g/l, 2×10 −6 g/l, 10 −7 g/l, 10 −7 g/l, 10 −8 g/l, 2×10 −9 g/l, or 10 −9 g/l, including ranges between any two of the listed values, for example, 10 −9 -10 −6 g/l, 10 −9 -10 −3 g/l, 10 −9 -10 −2 g/l, 10 −9 -0.1 g/l, 10 −9 -0.5 g/l, 10 −9 -0.9 g/l, 10 9 -0.09 g/l, 10 −6 -10 −3 g/l, 10 −6 -10 −2 g/l, 10 −6 -0.1 g/l, 10 −6 -0.5 g/l, 10 −6 -0.9 g/l, 10 −9 -0.09 g/l, 2×10 −6 -10 −2 g/l, 2×10 −6 -0.1 g/l, 2×10 −6 -0.5 g/l, 2×10 −6 -0.9 g/l, 2×10 −3 -0.09 g/l, 10 −3 -10 −2 g/l, 10 −3 -0.1 g/l, 10 −3 -0.5 g/l, 10 −3 -0.9 g/l, 10 −3 -0.09 g/l, 0.002-0.01 g/l, 0.002-0.1 g/l, 0.002-0.5 g/l, 0.002-0.9 g/l, 0.002-0.09 g/l, 0.01 g/l-0.99 g/l, 0.01 g/l-0.9 g/l, 0.01 g/l-0.5 g/l, 0.01 g/l-0.2 g/l, 0.01 g/l-0.1 g/l, 0.1 g/l-0.99 g/l, 0.1 g/l-0.9 g/l, 0.1 g/l-0.5 g/l, 0.1 g/l-0.2 g/l, 0.5 g/l-0.99 g/l, 0.5 g/l-0.9 g/l, and 0.5 g/l-0.7 g/l.

5 . The bone marrow container of claim 1 , wherein the TLR2 ligand is selected from the group consisting of hyaluronon, hyaluronic acid, monosodium urate crystals, biglycan, endoplasmin, HMGB1, HSP60, HSP70, human cardiac myosin, zymosan, lipoteichoic acids, peptidoglycans, and Pam2CSK4, and Pam3CSK4.

6 . The bone marrow container of claim 1 , wherein the non-ionic hydrophilic branched polysaccharide comprises poly(sucrose-co-epichlorhydrin) at a density of less than 1 gram per liter, and wherein the TLR2 ligand comprises hyaluronic acid.

7 . The bone marrow container of claim 1 , further comprising an integral delivery device.

8 . The bone marrow container of claim 1 , further comprising dextran.

9 . A kit comprising the bone marrow container of claim 1 , and a bone marrow aspiration needle.

10 . A separation system comprising:

A chassis comprising a cavity configured to receive a bone marrow container, the chassis comprising a track; and

a shaft disposed on the track, wherein the shaft is configured to move along the track, thereby compressing the bone marrow container when the bone marrow container is disposed in the chassis.