Granted Patent
B2
US 12,312,629 · App. 18/342,601 · Granted May 27, 2025
Polyhydroxyalkanoate production methods and materials and microorganisms used in same
Assignee:
Newlight Technologies, Inc.
View Patent ↗
Loading inventors, assignments & file history…
Abstract
Embodiments of the invention relate generally to methods to generate microorganisms and/or microorganism cultures that exhibit the ability to produce polyhydroxyalkanoates (PHA) from carbon sources at high efficiencies. In several embodiments, preferential expression of, or preferential growth of microorganisms utilizing certain metabolic pathways, enables the high efficiency PHA production from carbon-containing gases or materials. Several embodiments relate to the microorganism cultures, and/or microorganisms isolated therefrom.
Claims (24)
1. A method for modifying a functional characteristic of a polyhydroxyalkanoate (PHA) material, the method comprising:
providing a PHA comprising carbon and a biomass comprising carbon derived from one or more carbon-containing gasses;
subjecting the PHA and/or the biomass to a processing step in order to render the at least a portion of the biomass miscible with the PHA;
combining the PHA and the biomass in a mixture to form a compound, wherein the ratio of the biomass to the PHA in said PHA is between 1:1000 and 1000:1;
heating the compound to between 40 degrees Celsius and 200 degrees Celsius; and
causing, in response to the heating, the biomass to effect a functional modification of a first polymer and a second polymer of the PHA, wherein the functional modification comprises an increase in plasticization, nucleation, compatibilization, melt flow modification, strengthening, reduction of PHA crystallinity or rate of crystallization, increase in optical clarity, and/or elasticization.
2. The method of claim 1 , further comprising pressurizing the compound to between 1 atmosphere and 350 atmospheres.
3. The method of claim 1 , further comprising treating the PHA and/or the biomass with one or more of treatments selected from a group consisting of: heat, shear, pressure, solvent extraction, washing, filtration, centrifugation, sonication, enzymatic treatment, super critical material treatment, cellular dissolution, flocculation, acid and/or base treatment, drying, lysing, and chemical treatment.
4. The method of claim 1 , further comprising providing a medium comprising the biomass capable of metabolizing a source of carbon.
5. The method of claim 1 , wherein the biomass comprises one or more of methanotrophic, autotrophic, and heterotrophic biomass.
6. The method of claim 1 , wherein the biomass is present in the compound at a concentration of more than about 0.001%.
7. The method of claim 1 , wherein the first polymer is PHB, PHBV, PHHX, PHO, or other PHA polymer and the second polymer is a type of polyolefin.
8. A method for modifying a functional characteristic of a polyhydroxyalkanoate (PHA) material, the method comprising:
providing a PHA comprising carbon and a biomass comprising carbon derived from one or more carbon-containing gasses;
subjecting the PHA and/or the biomass to a processing step in order to render the at least a portion of the biomass miscible with the PHA;
combining the PHA and the biomass in a mixture to form a compound, wherein the ratio of the biomass to the PHA in said PHA is between 1:1000 and 1000:1;
heating the compound to between 40 degrees Celsius and 200 degrees Celsius; and
causing, in response to the heating, the biomass to effect a functional modification of a first polymer and a second polymer of the PHA, wherein the functional modification comprises an increase in nucleation and/or reduction of PHA crystallinity or rate of crystallization.
9. The method of claim 8 , further comprising pressurizing the compound to between 1 atmosphere and 350 atmospheres.
10. The method of claim 8 , further comprising treating the PHA and/or the biomass with one or more of treatments selected from a group consisting of: heat, shear, pressure, solvent extraction, washing, filtration, centrifugation, sonication, enzymatic treatment, super critical material treatment, cellular dissolution, flocculation, acid and/or base treatment, drying, lysing, and chemical treatment.
11. The method of claim 8 , further comprising providing a medium comprising the biomass capable of metabolizing a source of carbon.
12. The method of claim 8 , wherein the biomass comprises one or more of methanotrophic, autotrophic, and heterotrophic biomass.
13. The method of claim 8 , wherein the biomass is present in the compound at a concentration of more than about 0.001%.
14. The method of claim 8 , wherein the first polymer is PHB, PHBV, PHHX, PHO, or other PHA polymer and the second polymer is a type of polyolefin.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 13, 2024
From: HERREMA, MARKUS D.
To: NEWLIGHT TECHNOLOGIES, INC.
Reel/Frame 069356/0416 →
Continuity (8)
Continuation
13802622
· Mar 13, 2013
Provisional Application
61617534
· Mar 29, 2012
References Cited (227)
US 3275610A
· Coty
· 1966
[cited by applicant]
US 3878305A
· Damico et al.
· 1975
[cited by applicant]
US 4101533A
· Lafferty et al.
· 1978
[cited by applicant]
US 4375515A
· Patel et al.
· 1983
[cited by applicant]
US 4433053A
· Hughes et al.
· 1984
[cited by applicant]
US 4524569A
· Hanna
· 1985
[cited by applicant]
US 4562245A
· Stageman
· 1985
[cited by applicant]
US 4968611A
· Traussnig et al.
· 1990
[cited by applicant]
US 5250430A
· Peoples et al.
· 1993
[cited by applicant]
US 5292860A
· Shiotani et al.
· 1994
[cited by applicant]
US 5344766A
· Ramachandran et al.
· 1994
[cited by applicant]
US 5378621A
· Lawlis et al.
· 1995
[cited by applicant]
US H1430H
· Apel et al.
· 1995
[cited by applicant]
US 5434062A
· Groleau et al.
· 1995
[cited by applicant]
US 5480794A
· Peoples et al.
· 1996
[cited by applicant]
US 5487834A
· Carman et al.
· 1996
[cited by applicant]
US 5642630A
· Abdelmalek et al.
· 1997
[cited by applicant]
US 5691174A
· Liddell et al.
· 1997
[cited by applicant]
US 5723730A
· Montgomery et al.
· 1998
[cited by applicant]
US 5727903A
· Borray et al.
· 1998
[cited by applicant]
US 5747584A
· Noda
· 1998
[cited by applicant]
US 5789536A
· Liggat et al.
· 1998
[cited by applicant]
US 5842357A
· Siwajek et al.
· 1998
[cited by applicant]
US 5849894A
· Clemente et al.
· 1998
[cited by applicant]
US 5871980A
· Naylor et al.
· 1999
[cited by applicant]
US 5894062A
· Liddell
· 1999
[cited by applicant]
US 5942597A
· Noda et al.
· 1999
[cited by applicant]
US 6043063A
· Kurdikar et al.
· 2000
[cited by applicant]
US 6051411A
· Tartakovsky et al.
· 2000
[cited by applicant]
US 6096810A
· Asrar et al.
· 2000
[cited by applicant]
US 6156852A
· Asrar et al.
· 2000
[cited by applicant]
US 6201083B1
· Asrar et al.
· 2001
[cited by applicant]
US 6205704B1
· Schmitz et al.
· 2001
[cited by applicant]
US 6248862B1
· Asrar et al.
· 2001
[cited by applicant]
US 6368836B2
· Horowitz et al.
· 2002
[cited by applicant]
US 6395520B1
· Babel et al.
· 2002
[cited by applicant]
US 6446385B1
· Crutcher
· 2002
[cited by applicant]
US 6472188B1
· Lee et al.
· 2002
[cited by applicant]
US 6599423B2
· Boles et al.
· 2003
[cited by applicant]
US 6620869B2
· Asrar et al.
· 2003
[cited by applicant]
US 6666027B1
· Cardenas, Jr.
· 2003
[cited by applicant]
US 6709848B1
· Martin et al.
· 2004
[cited by applicant]
US 6709849B2
· Cheung
· 2004
[cited by applicant]
US 6749368B2
· Ankeny et al.
· 2004
[cited by applicant]
US 6770464B2
· Steinbuechel et al.
· 2004
[cited by applicant]
US 6982161B1
· Herrema
· 2006
[cited by applicant]
US 7098298B2
· Kinoshita et al.
· 2006
[cited by applicant]
US 7141400B2
· Yu
· 2006
[cited by applicant]
US 7208535B2
· Asrar et al.
· 2007
[cited by applicant]
US 7226765B2
· Narasimhan et al.
· 2007
[cited by applicant]
US 7410717B2
· Moon et al.
· 2008
[cited by applicant]
US 7455999B2
· Madison et al.
· 2008
[cited by applicant]
US 7504556B2
· Madison et al.
· 2009
[cited by applicant]
US 7524659B2
· Nomoto et al.
· 2009
[cited by applicant]
US 7527963B2
· Nomoto et al.
· 2009
[cited by applicant]
US 7579176B2
· Herrema et al.
· 2009
[cited by applicant]
US 7641706B1
· McMurry et al.
· 2010
[cited by applicant]
US 7745197B1
· Herrema et al.
· 2010
[cited by applicant]
US 7887893B2
· Billington et al.
· 2011
[cited by applicant]
US 8030021B2
· Criddle et al.
· 2011
[cited by applicant]
US 8071342B2
· Herrema et al.
· 2011
[cited by applicant]
US 8177870B2
· Herrema et al.
· 2012
[cited by applicant]
US 8263373B2
· Herrema et al.
· 2012
[cited by applicant]
US 8465876B2
· Herrema et al.
· 2013
[cited by applicant]
US 8703470B2
· Herrema et al.
· 2014
[cited by applicant]
US 8735112B2
· Verwaal et al.
· 2014
[cited by applicant]
US 8735113B2
· Herrema et al.
· 2014
[cited by applicant]
US 8930236B2
· Gillenson et al.
· 2015
[cited by applicant]
US 8945915B2
· Herrema et al.
· 2015
[cited by applicant]
US 9040267B2
· Herrema
· 2015
[cited by applicant]
US 9085784B1
· Herrema
· 2015
[cited by applicant]
US 9243266B2
· Herrema et al.
· 2016
[cited by applicant]
US 9725744B2
· Herrema
· 2017
[cited by applicant]
US 9850508B2
· Herrema et al.
· 2017
[cited by applicant]
US 9868967B2
· Herrema et al.
· 2018
[cited by applicant]
US 20030004299A1
· Srienc et al.
· 2003
[cited by applicant]
US 20030217648A1
· Noda et al.
· 2003
[cited by applicant]
US 20070141660A1
· Chotani et al.
· 2007
[cited by applicant]
US 20070161097A1
· Green et al.
· 2007
[cited by applicant]
US 20070192221A1
· Sandor et al.
· 2007
[cited by applicant]
US 20090176900A1
· Hirose et al.
· 2009
[cited by applicant]
US 20090203093A1
· Steinbuechel et al.
· 2009
[cited by applicant]
US 20090301099A1
· Nigro
· 2009
[cited by applicant]
US 20100093043A1
· Huisman et al.
· 2010
[cited by applicant]
US 20100190224A1
· Poetter et al.
· 2010
[cited by applicant]
US 20100255540A2
· Herrema
· 2010
[cited by applicant]
US 20100330382A1
· Dou et al.
· 2010
[cited by applicant]
US 20110112257A1
· Billington et al.
· 2011
[cited by applicant]
US 20110146488A1
· Jacob
· 2011
[cited by applicant]
US 20110159556A1
· Pieja et al.
· 2011
[cited by applicant]
US 20120028321A1
· Criddle et al.
· 2012
[cited by applicant]
US 20120077254A1
· Morse et al.
· 2012
[cited by applicant]
US 20120165500A1
· Herrema et al.
· 2012
[cited by applicant]
US 20130023674A1
· Narayan et al.
· 2013
[cited by applicant]
US 20130071890A1
· Criddle et al.
· 2013
[cited by applicant]
US 20140065311A1
· Moseley et al.
· 2014
[cited by applicant]
US 20140256026A1
· Herrema et al.
· 2014
[cited by applicant]
US 20150140621A1
· Herrema et al.
· 2015
[cited by applicant]
US 20170268026A1
· Herrema
· 2017
[cited by applicant]
US 20200263212A1
· Herrema
· 2020
[cited by applicant]
US 20200347417A1
· Herrema
· 2020
[cited by applicant]
US 20210340581A1
· Herrema et al.
· 2021
[cited by applicant]
US 20230265237A1
· Coragliotti et al.
· 2023
[cited by applicant]
US 20230272158A1
· Leon et al.
· 2023
[cited by applicant]
EP 0694074B1
· 1997
[cited by applicant]
JP 2003184575A
· 2003
[cited by applicant]
KR 1020040056723A
· 2004
[cited by applicant]
WO 9722654A1
· 1997
[cited by applicant]
WO 9957298A1
· 1999
[cited by applicant]
WO 2005047415A1
· 2005
[cited by applicant]
WO 2007004484A1
· 2007
[cited by applicant]
WO 2007024255A1
· 2007
[cited by applicant]
WO 2007149418A2
· 2007
[cited by applicant]
WO 2008103134A2
· 2008
[cited by applicant]
WO 2009129499A1
· 2009
[cited by applicant]
WO 2010008445A2
· 2010
[cited by applicant]
WO 2010008447A1
· 2010
[cited by applicant]
WO 2010047052A1
· 2010
[cited by applicant]
WO 2011031566A1
· 2011
[cited by applicant]
WO 2012028210A1
· 2012
[cited by applicant]
WO 2012122343A2
· 2012
[cited by applicant]
WO 2021002092A1
· 2021
[cited by applicant]
WO 2022010940A1
· 2022
[cited by applicant]
WO 2022015539A1
· 2022
[cited by applicant]
WO 2022173465A1
· 2022
[cited by applicant]
WO 2022173466A1
· 2022
[cited by applicant]
Stanley, et al., Copper stress underlies the fundamental chan 1e in intracellular location of meU1ane mono-oxygenase in methane-oxidizing organisms: Studies in batct1 and continuous cultures., Biotech Letters, vol. 5(7)…
[cited by applicant]
Tellez, et al., Isolation of copper biochelates from Methylosinus trichosporiurn 0B3b and soluble methane monooxygenase mutants, App. and Env. Microbial., vol. 64(3):1115-1122 (1998).
[cited by applicant]
Tyson et al., Tunnel Ventilation for Tie Stall Dairy Barns, Penn State, College of Agricultural Sciences, Agricultural and Biological Engineering, 2nd Edition Jan. 1, 2004.
[cited by applicant]
Verlinden et al., Bacterial synthesis of biodegradable polyhydroxyalkanoates, Journal of Applied Microbiology, vol. 102, 2007, pp. 1437-1449.
[cited by applicant]
Wendlandt et al., “Possibilities for controlling a PHB accumulation process using various analytical methods,” J. of Biotechn. 2005, vol. 117, pp. 119-129.
[cited by applicant]
Wendlandt et al., “Producing poly-3-hydroxybutyrate with a high molecular mass from methane,” J. Biotechnol. 2001, vol. 86, pp. 127-133, see pp. 127-128.
[cited by applicant]
Wendlandt et al., “The potential of methane-oxidizing bacteria for applications in environmental biotechnology”, Engineering in Life Sciences, vol. 10, No. 2, pp. 87-102, 2010.
[cited by applicant]
Zhang et al.Biosynthesis of poly-3-hydroxybutyrate with a high molecular weight by methanotrophfrom methane and methanol.Joural of Natural Gas Chemistry, vol. 17, pp. 103-109 (2008).
[cited by applicant]
Ansejo et al. “Microbial conversion of methane into poly-beta-hydroxybutyrate (PHB) growth and intracellular product accumulation in a type II methanotroph” J. Ferment Technol., 1986, vol. 64, No. 4, pp. 271-278.
[cited by applicant]
Bothe, Harald, et al. “Heterotrophic bacteria growing in association with Methylococcus capsulatus (Bath) in a single cell production porocess”, Applied Microbiology and Biotechnology, Springer, DE vol. 59, No. 1, Jun. …
[cited by applicant]
Brigman, Methanotrophic Bacteria: Use in Bioremediation, Westinghouse Savannah River Company, on-line publication No. WSRC-MS-2001-00058, http://sti.srs.gov/fulltext/ms2001058/ms2001058.html,2001.
[cited by applicant]
D'Aquino, “Methane to Protein,” at http://www.aptagen.com/corporate/AptagenDocuments/Articles/che.html, Oct. 27, 2000.
[cited by applicant]
Gerlagh, T, Matter 2.0, “A module characterization for the agriculture and food sector” (Jul. 1999). 5.1.1 Enteric fermentation (p. 22). ftp://ftp.ecn.nl/pub/www/library/report/1999/c99048.pdf.
[cited by applicant]
International Preliminary Report on Patentability, re PCT Application No. PCT/US12/028210, dated Sep. 19, 2013.
[cited by applicant]
International Search Report and Written Opinion of the International Searching Authority for International Application No. PCT/US2010/047052 mailed on Dec. 27, 2010.
[cited by applicant]
Invitation to Pay Additional Fees, re PCT Application No. PCT/US07/004484, dated Jul. 16, 2008.
[cited by applicant]
Invitation to Pay Additional Fees, re PCT Application No. PCT/US10/047052, dated Oct. 8, 2010.
[cited by applicant]
Invitation to Pay Additional Fees, re PCT Application No. PCT/US12/028210, dated Jun. 15, 2012.
[cited by applicant]
Jensen, S. et al., “Methanol Improves Methane Uptake in Starved Methanotrophic Microorganisms” Applied and Environmental Microbiology, (1998) vol. 64(3): 1143-1146.
[cited by applicant]
Nori, “A blockchain-based marketplace 1or removing carbon dioxide from the atmosphere,” Version 3.0, Aug. 27, 2018.
[cited by applicant]
Pieja et al., Poly-3-Hydroxybutyrate Metabolism in the Type II Methanotroph Methylocystis parvus OBBP, Applied and Environmental Microbiology, Sep. 2011, vol. 77(17), p. 6012-6019.
[cited by applicant]
Alvarado et al., Effects of natural porous additives on the tensile mechanical perforamnceand moisture absorption behavior of PHBV-based composites for construction, Stanford Undergraduate Research Journal, Spring 2011,…
[cited by applicant]
Bartle, “Exploring a Gaseating Bacteria,” University of Bergen Magazine, 2002, at http://www.uib.no/elin/elpub/uibmag/en02/bacteria.htmle.
[cited by applicant]
Bioremediation—field Experience: Field Experience, Paul E. Flathman, Douglas E. Jerger and Jurgen H. Exner, CRC Press, Boca Raton, Florida, 1994, pp. 275-276.
[cited by applicant]
Bourne et al. “Comparison of pmoA PCR Primer Sets as Tools for Investigating Methanotroph Diversity in Three Danish Soils”, Applied and Environmental Microbiology, Sep. 2001. p. 3802-3809.
[cited by applicant]
Burrows et al., Substrate Specificites of the Soluble and Particulate Methane Monooxygenases of Methylosinus-Trichosporium OB03B, J. Gen. Microbiol. vol. 130 (12):3327-3333 (1984).
[cited by applicant]
Christian et al., Sustainable Biocomposites for Construction, Composites & Polycon 2009, American Composites Manufacturers Association, Jan. 15-17, 2009, Tampa, Florida, pp. 1-6.
[cited by applicant]
Climate Change 2001: Working Group I: The Scientific Basis Intergovernmental Panel on Climate Change, http://www.grida.no/climate/ipcc_tar/wg1/017.htm._2001.
[cited by applicant]
Cow Power, htt://www.riverdeep.net/current/2002/03/032502t_cowpower.jhtml, Mar. 2002.
[cited by applicant]
Deublein et al. “Biagas from Waste and Renewable Resources”, p. V-XIII, Part III, Chapter 2.1.1, p. 94, WILEY-VCH Verlag Gmbh & Co. KgaA, Weinheim, 2008.
[cited by applicant]
Dias et al., “Recent Advances in Polyhydroxyalkanoate Production by Mixed Aerobic Cultures: From the Substrate to the Final Product.” Macromol. Biosci. (2006) 6, 885-906.
[cited by applicant]
Eckard, Richard, “The abatement challenge for Australian Agriculture” Greenhouse in Agriculture, The University of Melbourne and Department of Primary Industries, Victori (2007). Enteric methane (p. 2). http://www.dpc.v…
[cited by applicant]
Fogel et al., Biodegradation of Chlorinated Ethenes by a Methane-Utilizing Mixed Culture, Applied and Environmental Microbiology, vol. 51(4):720-724 (1986).
[cited by applicant]
Frans-Jaco et al., “Spatial Distribution and Inhibition by Ammonium of Methane Oxidation in Intertidal Freshwater Marshes” Applied and Environmental Microbiology, (1997) vol. 63(12): 4734-4740.
[cited by applicant]
Frigon et al. “rRNA and Poly—Hydroxybutyrate Dynamics in Bioreactors Subjected to Feast and Famine Cycles” Applied and Environmental Microbiology, Apr. 2006, p. 2322-2330.
[cited by applicant]
Gasser, “Agricultural productivity and the nitrogen cycle,” Phil Trans R Soc Lond 8296;303-314, 1982.
[cited by applicant]
Gay, S.W., “Natural ventilation for freestall dairy barns,” Pub. No. 442-763, Virginia Cooperative Extension, Virginia Polytechnic institute and State university, http://www.ext.vt.edu/pubs/442-763/442-763.pdf, 2002.
[cited by applicant]
Gooch, Natural or Tunnel Ventilation of Freestall Structures: What is Right for Your Dairy Facility? www.milkproduct.com, Published Nov. 4, 2005.
[cited by applicant]
Graham, et al. Factors affecting competition between type 1 and type 2 methanotrophs in two organism, continuous-flow reactors. Microb Ecol (1993) vol. 25 p. 1-17.
[cited by applicant]
Helm et al. Characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolmer in an open system. Journal of Applied Microbiology, vol. 101, pp. 387-395 (2006).
[cited by applicant]
Helm et al. Potassium deficiency results in accumulation of ultra-high molecular weight poly-beta-hydroxybutyrate in a methane-utilizing mixed culture. Journal of Applied Microbiology, vol. 105, pp. 1054-1061, 2008.
[cited by applicant]
Helm J., Methanotrophic bacteria as producers of poly(beta-hydroxybutyric acid) (PHB)—characterization of the process, the polymer and the stable mixed culture, Tech. Univ. Desden, Faculty of Mechanical Engineering, doc…
[cited by applicant]
Henrysson et al., “Influence of the endogenous storage lipid poly-[3-hydroxybutyrate on the reducing power availability during cometabolism of trichloroethylene and naphthalene by resting methanotrophic mixed cultures,”…
[cited by applicant]
How can livestock methane emissions be reduced? Ruminant Livestock (Mar. 2007). http://www.epa.gov/methane/rlep/faq.html.
[cited by applicant]
International Preliminary Report on Patentability and Written Opinion of the International Searching Authority for International Application No. PCT/US07/04484 mailed on Sep. 3, 2009.
[cited by applicant]
International Preliminary Report on Patentability for International Application No. PCT/US05/47415 dated Feb. 20, 2008.
[cited by applicant]
International Preliminary Report on Patentability, re PCT Application No. PCT/US2010/047052, dated Mar. 8, 2012.
[cited by applicant]
International Search Report and Written Opinion of PCT Application No. PCT/US05/47415, dated Aug. 9, 2006.
[cited by applicant]
International Search Report and Written Opinion of PCT Application No. PCT/US07/04484, dated Sep. 26, 2008.
[cited by applicant]
International Search Report and Written Opinion of PCT Application No. PCT/US2012/028210, Mailed on Aug. 23, 2012.
[cited by applicant]
International Search Report and Written Opinion, for International Application No. PCT/US2020/036986, mailed Aug. 26, 2020, in 15 pages.
[cited by applicant]
Johnson et al., “Methane emissions from cattle,” J. Anim. Sci. 73:2483-2492, 1995.
[cited by applicant]
Jolley, Ainsley, Technologies for Reducing Non-Energy-Related Emissions (Mar. 2006). Enteric Fermentation (p. 8). http://www.cfses.com/documents/climate/10_%20Jolley_Technologies_for_Reducing_Non-energy_Related_Emission…
[cited by applicant]
Lee et al., “High-density algal photobioreactors using light-emitting diodes” Biotechnology and Bioengineering, Nov. 20, 1994, vol. 44(10), pp. 1161-1167.
[cited by applicant]
Madden et al. Introducing the Carbon Impact Factor. thejei.com. Jan. 31, 2016 (Jan. 31, 2016) [retrieved on Aug. 3, 2020]. Retrieved from the internet <URL: http:l/www.thejei.com/introducing-the-carbon-impact-factor/> e…
[cited by applicant]
McDonald et al., “The Soluble Methane Monooxygenase Gene Cluster of the Trichloroethylene-Degrading Methanotroph
[cited by applicant]
Meeting Minutes of Methane to Markets, Agriculture Task Force Meeting dated Jun. 22, 2005. http://www.methanetomarkets.org/resources/ag/docs/ag-meeting.pdf.
[cited by applicant]
Methane Emissions from Livestock Enteric Fermentation (p. 150). Reducing Emissions of Non-CO2 Greenhouse Gases (Sep. 2006). http://www. climatetechnology. gov/stratplan/final/CCTP-StratPlan- Ch07-Sep-2006.pdf.
[cited by applicant]
Muller. et al., “Adaptive responses of Ralstonia eutropha to feast and famine conditions analysed by flow cytometry,” J Biotechnol, vol. 75, Issue 2-3, Oct. 8, 1999, pp. 81-97.
[cited by applicant]
Murrell et al. “Regulation of expression of methane monooxygenases by copper ions,” Trends in Microbiology 8(5):221-225, 2000.
[cited by applicant]
Nichols, Peter D., and White, D.C., “Accumulation of poly-B-hydroxybutyrate in a methane-enriched, halogenated hydrocarbon-degrading soil column implications for microbial community structure and nutritional status”. Hy…
[cited by applicant]
Norferm's future under discussion; Scandinavian Oil-Gas Magazine; http://www.scandoil.com/moxie-bm2/news/company news/norferms-future-under-dis.shtml, Published Oct. 27, 2005.
[cited by applicant]
Pfluger et al. Selection of Type I and Type II methanotropic proteobacteria in a fluidized bed reactor under non-sterile conditions. Bioresource Technology, vol. 102, pp. 9919-9926, 2011 (available online Aug. 19, 2011).
[cited by applicant]
Pieja et al., Distribution and Selection of Poly-3-Hydroxybutyrate Production Capacity in Methanotrophic Proteobacteria, Microb Ecol (2011), vol. 62, pp. 564-573.
[cited by applicant]
Polakovic, “Getting the Cows to Cool It,” Los Angeles Times, Jun. 7, 2003, pp. A1-A17, Los Angeles, CA U.S.A.
[cited by applicant]
Potential management practices and technologies for reducing methane emissions from agriculture. http://www.maf.govt.nz/mafnet/rural-nz/sustainable-resource-use/climate/g-reen-house-gas-migration/ghg-mitigation-05.html …
[cited by applicant]
Reddy, et al., Polyhydroxyalkanoates: An Overview, Bioresource Technology, vol. 87: 137-146 (2003).
[cited by applicant]
Reis, et al. “Production of polyhydroxyalkanoates by mixed microbial cultures” Bioprocess and Biosystems Engineering, vol. 25, No. 6, 377-385, DOI; 10.1007/s00449-003-0322-4, (2003).
[cited by applicant]
Shah, et al. “Batch Cultivation of Methylosinus trichosporium OB3b: V. Characterization of Poly-B-Hydroxybutyrate Production Under Methane-Dependent Growth Conditions” Biotechnology and Bioengineering, vol. 49, pp. 161-…
[cited by applicant]
Sherry, “How Blockchain Can Make Carbon Markets More Accessible.” ecosystemmarketplace.com. Oct. 2, 2018, in 3 pages, available at https://www.ecosystemmarketplace.com/articles/how-blockchain-can-make-carbon-markets-mor…
[cited by applicant]
Singh et al., “Bacillus subtilis as potential producer for polyhydroxyalkanoates.” BioMed Central. Microbial Cell Factories. Jul. 20, 2009, vol. 8, No. 38, pp. 1-11.
[cited by applicant]
Cited By (1)