IP Library › Patent Application 18364273
Patent Application
App. No. 18/364,273

WATER-SOLUBLE CANNABINOID FOOD AND BEVERAGE ADDITIVE HAVING ENHANCED STABILITY

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Patent No.
US None
App. No.
18/364,273
Abstract

The present invention includes systems, methods and compositions related to the production and use of chemically modified cannabinoid food and beverage additives having enhanced water solubility and stability.

Claims (24)

1 . A food additive comprising:

an aqueous solution;

a cannabinoid glycoside dissolved in said aqueous solution; and

wherein the cannabinoid glycoside is generated by incubating a quantity of a cannabinoid in a suspension cell culture of genetically modified yeast cells expressing a heterologous nucleotide sequence, operably linked to a promotor, encoding a heterologous glycosyltransferase having activity toward the cannabinoid; and

wherein the cannabinoid glycoside is selected from: cannabidiol (CBD) glycoside, cannabidiolic acid (CBDA) glycoside, or a combination of the same.

2 . The composition of claim 1 , wherein said heterologous glycosyltransferase is selected from: NtGT3; NtGT4; and NtGT5, or a fragment thereof.

3 . The composition of claim 1 , wherein said heterologous glycosyltransferase is selected from: SEQ ID NO. 7, SEQ ID NO. 27, SEQ ID NO. 29, SEQ ID NO. 31, SEQ ID NO. 33, SEQ ID NO. 35, SEQ ID NO. 37, SEQ ID NO. 39, SEQ ID NO. 55, SEQ ID NO. 57, SEQ ID NO. 59, SEQ ID NO. 61, and SEQ ID NO. 63, or a fragment thereof.

4 . The composition of claim 1 , wherein said heterologous glycosyltransferase is selected from: a heterologous glycosyltransferase having at least 90% sequence identity with SEQ ID NO. 7, at least 90% sequence identity with SEQ ID NO. 27, at least 90% sequence identity with SEQ ID NO. 29, at least 90% sequence identity with SEQ ID NO. 31, at least 90% sequence identity with SEQ ID NO. 33, at least 90% sequence identity with SEQ ID NO. 35, at least 90% sequence identity with SEQ ID NO. 37, at least 90% sequence identity with SEQ ID NO. 39, at least 90% sequence identity with SEQ ID NO. 55, at least 90% sequence identity with SEQ ID NO. 57, at least 90% sequence identity with SEQ ID NO. 59, at least 90% sequence identity with SEQ ID NO. 61, or at least 90% sequence identity with SEQ ID NO. 63.

5 . The composition of claim 1 , wherein said aqueous solution is selected from: water, saline, purified water, propylene glycol, deionized water, or combinations thereof.

6 . The composition of claim 1 , wherein said aqueous solution has a pH at or below 7.4.

7 . The composition of claim 1 , wherein said cannabinoid glycoside comprises an acetylated cannabinoid glycoside generated by incubating the cannabinoid with the yeast cell culture.

8 . A consumable food additive comprising:

a dehydrated powder or liquid containing an isolated cannabinoid glycoside selected from: cannabidiol (CBD) glycoside, cannabidiolic acid (CBDA) glycoside, tetrahydrocannabinol (THC) glycoside, tetrahydrocannabinolic acid (THCA) glycoside, or a combination of the same;

wherein the cannabinoid glycoside is generated by incubating a quantity of a cannabinoid selected from CBD, CBDA, THC, THCA, or a combination of the same, in a suspension cell culture of genetically modified yeast cells expressing a heterologous nucleotide sequence, operably linked to a promotor, encoding a heterologous glycosyltransferase having activity toward the cannabinoid.

9 . The composition of claim 8 , wherein said heterologous glycosyltransferase comprises a glycosyltransferase protein from tobacco selected from the group consisting of: NtGT3; NtGT4; and NtGT5, or a fragment thereof.

10 . The composition of claim 8 , wherein said heterologous glycosyltransferase comprises a heterologous glycosyltransferase selected from the group of consisting of: SEQ ID NO. 7, SEQ ID NO. 27, SEQ ID NO. 29, SEQ ID NO. 31, SEQ ID NO. 33, SEQ ID NO. 35, SEQ ID NO. 37, SEQ ID NO. 39, SEQ ID NO. 55, SEQ ID NO. 57, SEQ ID NO. 59, SEQ ID NO. 61, and SEQ ID NO. 63, or a fragment thereof.

11 . The composition of claim 8 , wherein said heterologous glycosyltransferase comprises a heterologous glycosyltransferase having at least 90% sequence identity with SEQ ID NO. 7, at least 90% sequence identity with SEQ ID NO. 27, at least 90% sequence identity with SEQ ID NO. 29, at least 90% sequence identity with SEQ ID NO. 31, at least 90% sequence identity with SEQ ID NO. 33, at least 90% sequence identity with SEQ ID NO. 35, at least 90% sequence identity with SEQ ID NO. 37, at least 90% sequence identity with SEQ ID NO. 39, at least 90% sequence identity with SEQ ID NO. 55, at least 90% sequence identity with SEQ ID NO. 57, at least 90% sequence identity with SEQ ID NO. 59, at least 90% sequence identity with SEQ ID NO. 61, or at least 90% sequence identity with SEQ ID NO. 63.

12 . The composition of claim 8 , wherein said cannabinoid glycoside comprises an acetylated cannabinoid glycoside generated by incubating the cannabinoid with the yeast cells.

13 . A consumable food additive comprising:

a dehydrated powder or liquid containing an isolated acetylated cannabinoid glycoside selected from: acetylated cannabidiol (CBD) glycoside, acetylated cannabidiolic acid (CBDA) glycoside, acetylated tetrahydrocannabinol (THC) glycoside, acetylated tetrahydrocannabinolic acid (THCA) glycoside, or a combination of the same;

wherein the acetylated cannabinoid glycoside is generated by incubating a quantity of a cannabinoid selected from: CBD, CBDA, THC, THCA or a combination of the same, in a suspension cell culture of genetically modified yeast cells expressing a heterologous nucleotide sequence, operably linked to a promotor, encoding a heterologous glycosyltransferase having activity toward the cannabinoid.

14 . The composition of claim 13 , wherein said heterologous glycosyltransferase comprises a glycosyltransferase protein from tobacco selected from the group consisting of: NtGT3; NtGT4; and NtGT5, or a fragment thereof.

15 . The composition of claim 13 , wherein said heterologous glycosyltransferase comprises a heterologous glycosyltransferase selected from the group of consisting of: SEQ ID NO. 7, SEQ ID NO. 27, SEQ ID NO. 29, SEQ ID NO. 31, SEQ ID NO. 33, SEQ ID NO. 35, SEQ ID NO. 37, SEQ ID NO. 39, SEQ ID NO. 55, SEQ ID NO. 57, SEQ ID NO. 59, SEQ ID NO. 61, and SEQ ID NO. 63, or a fragment thereof.

16 . The composition of claim 13 , wherein said heterologous glycosyltransferase comprises a heterologous glycosyltransferase having at least 90% sequence identity with SEQ ID NO. 7, at least 90% sequence identity with SEQ ID NO. 27, at least 90% sequence identity with SEQ ID NO. 29, at least 90% sequence identity with SEQ ID NO. 31, at least 90% sequence identity with SEQ ID NO. 33, at least 90% sequence identity with SEQ ID NO. 35, at least 90% sequence identity with SEQ ID NO. 37, at least 90% sequence identity with SEQ ID NO. 39, at least 90% sequence identity with SEQ ID NO. 55, at least 90% sequence identity with SEQ ID NO. 57, at least 90% sequence identity with SEQ ID NO. 59, at least 90% sequence identity with SEQ ID NO. 61, or at least 90% sequence identity with SEQ ID NO. 63.