Granted Patent
B2
US 12,467,898 · App. 18/391,244 · Granted Nov 11, 2025
Nanopore arrays for sequencing nucleic acids
Inventors:
Stuart William Reid (Oxford, GB); Terence Alan Reid (Oxford, GB); James Anthony Clarke (Oxford, GB); Steven Paul White (Oxford, GB); Gurdial Singh Sanghera (Oxford, GB)
Assignee:
Oxford Nanopore Technologies PLC
G01N27/44791B01L3/502707B01L3/50273C12Q1/6869G01N27/3278G01N27/453G01N33/48721B01L2300/0645B01L2300/161B01L2400/0421B01L2400/0427
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Abstract
To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.
Claims (29)
1 . A device, comprising:
a substrate;
a conductive layer disposed in a top portion of the substrate;
an electrode layer disposed on the conductive layer;
a dielectric layer disposed on the electrode layer;
a recess in the dielectric layer, the recess exposing a portion of the electrode layer and having a recess sidewall formed by the dielectric layer and a recess bottom on the exposed portion of the electrode layer; wherein the recess bottom is hydrophilic, a lower portion of the recess sidewall is hydrophilic, and an upper portion of the recess sidewall is hydrophobic.
2 . The device of claim 1 , wherein the recess has a width, wherein the width at different depths within each recess are not the same value.
3 . The device of claim 1 , wherein the recess has a width, wherein the width at different depths within each recess are the same value.
4 . The device of claim 1 , further comprising a layer of amphiphilic molecules.
5 . The device of claim 4 , wherein the layer of amphiphilic molecules comprises lipids.
6 . The device of claim 5 , wherein the lipids form a lipid bilayer.
7 . The device of claim 1 , further comprising a nanopore.
8 . The device of claim 7 , wherein the nanopore comprises a protein nanopore.
9 . The device of claim 8 , wherein the protein nanopores comprise alpha-hemolysin proteins.
10 . The device of claim 8 , wherein the protein nanopores are within biological membranes.
11 . The device of claim 1 , wherein the device comprises a semiconductor substrate in which electronic circuits is formed.
12 . The device of claim 11 , wherein the electronic circuits further comprise analog to digital converters, memory, or clock circuits.
13 . The device of claim 12 , further comprising conductive paths extending from the recess to the electronic circuits.
14 . The device of claim 1 , wherein the recess has a width, wherein the width at different depths within each recess vary up to about 5 μm.
15 . The device of claim 1 , wherein the electrode layer is silver/silver chloride.
16 . The device of claim 1 , wherein the top surface of the dielectric layer is hydrophobic.
17 . A method of polymer sequencing using the device of claim 1 .
18 . The method of claim 17 , wherein the method of polymer sequencing comprises nucleic acid molecule sequencing.
19 . The method of claim 18 , wherein the nucleic acid molecules comprise DNA.
20 . A device, comprising:
a substrate;
a conductive layer disposed in a top portion of the substrate;
a dielectric layer disposed on the conductive layer;
a recess in the dielectric layer, the recess exposing a portion of the conductive layer and having a recess sidewall formed by the dielectric layer and a recess bottom on the exposed portion of the conductive layer; wherein the recess bottom is hydrophilic, a lower portion of the recess sidewall is hydrophilic, and an upper portion of the recess sidewall is hydrophobic; wherein the portion of the conductive layer underneath the recess constitutes an electrode.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 20, 2023
From: REID, STUART WILLIAM; REID, TERRANCE ALAN; CLARKE, JAMES ANTHONY; WHITE, STEVEN PAUL; SANGHERA, GURDIAL SINGH
To: OXFORD NANOPORE TECHNOLOGIES LIMITED
Reel/Frame 065925/0200 →
CHANGE OF NAME
Recorded Dec 20, 2023
From: OXFORD NANOPORE TECHNOLOGIES LIMITED
To: OXFORD NANOPORE TECHNOLOGIES PLC
Reel/Frame 066123/0477 →
Priority Claims (1)
GB 0724736
· Dec 19, 2007
· national
Continuity (7)
References Cited (228)
US 3799743A
· Alexander et al.
· 1974
[cited by applicant]
US 4154795A
· Thorne
· 1979
[cited by applicant]
US 5234566A
· Osman et al.
· 1993
[cited by applicant]
US 5403451A
· Riviello et al.
· 1995
[cited by applicant]
US 5795782A
· Church et al.
· 1998
[cited by applicant]
US 6056922A
· Ikematsu
· 2000
[cited by applicant]
US 6300141B1
· Segal et al.
· 2001
[cited by applicant]
US 6479288B1
· Laffafian et al.
· 2002
[cited by applicant]
US 6503452B1
· Boxer et al.
· 2003
[cited by applicant]
US 6699697B2
· Klemic et al.
· 2004
[cited by applicant]
US 6863833B1
· Bloom et al.
· 2005
[cited by applicant]
US 6913697B2
· Lopez et al.
· 2005
[cited by applicant]
US 6916488B1
· Meier et al.
· 2005
[cited by applicant]
US 6936433B2
· Akeson et al.
· 2005
[cited by applicant]
US 7077939B1
· Crooks et al.
· 2006
[cited by applicant]
US 7144486B1
· Fritsch et al.
· 2006
[cited by applicant]
US 7169272B2
· Fritsch et al.
· 2007
[cited by applicant]
US 7745116B2
· Williams
· 2010
[cited by applicant]
US 7939270B2
· Holden et al.
· 2011
[cited by applicant]
US 8124191B2
· Ervin et al.
· 2012
[cited by applicant]
US 8461854B2
· Chen et al.
· 2013
[cited by applicant]
US 20030111340A1
· Cheng et al.
· 2003
[cited by applicant]
US 20050022895A1
· Barth et al.
· 2005
[cited by applicant]
US 20050230272A1
· Lee et al.
· 2005
[cited by applicant]
US 20060073489A1
· Li
· 2006
[cited by examiner]
US 20080254995A1
· Kim et al.
· 2008
[cited by applicant]
US 20090167288A1
· Reid et al.
· 2009
[cited by applicant]
US 20110005918A1
· Akeson
· 2011
[cited by examiner]
US 20110120871A1
· Reid et al.
· 2011
[cited by applicant]
US 20110121840A1
· Sanghera et al.
· 2011
[cited by applicant]
US 20110214991A1
· Kim et al.
· 2011
[cited by applicant]
US 20110287414A1
· Chen et al.
· 2011
[cited by applicant]
US 20120010085A1
· Rava et al.
· 2012
[cited by applicant]
US 20130140192A1
· Behrends et al.
· 2013
[cited by applicant]
US 20140296083A1
· Brown et al.
· 2014
[cited by applicant]
US 20140329693A1
· Reid et al.
· 2014
[cited by applicant]
US 20140346059A1
· Akeson
· 2014
[cited by applicant]
CN 1303147A
· 2001
[cited by applicant]
CN 1434461
· 2003
[cited by applicant]
CN 101078704
· 2007
[cited by applicant]
CN 101490277A
· 2009
[cited by applicant]
CN 203466320U
· 2013
[cited by applicant]
DE 102010022929A1
· 2011
[cited by applicant]
EP 0532215A2
· 1993
[cited by applicant]
EP 1120469A2
· 2001
[cited by applicant]
EP 1645628
· 2006
[cited by applicant]
EP 1669746A1
· 2006
[cited by applicant]
EP 1677102
· 2006
[cited by applicant]
EP 1688742
· 2006
[cited by applicant]
EP 1710578
· 2006
[cited by applicant]
EP 1712909A1
· 2006
[cited by applicant]
EP 1779921A1
· 2007
[cited by applicant]
EP 081606444
· 2008
[cited by applicant]
EP 2219032A1
· 2010
[cited by applicant]
GB 2237390
· 1991
[cited by applicant]
GB 2446823
· 2008
[cited by applicant]
JP S5274882A
· 1977
[cited by applicant]
JP 4127066A2
· 1990
[cited by applicant]
JP 4014773A2
· 1992
[cited by applicant]
JP 4215052
· 1992
[cited by applicant]
JP 7307172A2
· 1995
[cited by applicant]
JP 2004158330A2
· 2004
[cited by applicant]
JP 200598718
· 2005
[cited by applicant]
JP 2005539242
· 2005
[cited by applicant]
JP 2006312141
· 2006
[cited by applicant]
JP 2008194573
· 2008
[cited by applicant]
JP 2010186677A2
· 2010
[cited by applicant]
WO WO1994025862A1
· 1994
[cited by applicant]
WO WO1997016545A1
· 1997
[cited by applicant]
WO WO1998058248
· 1998
[cited by applicant]
WO WO2000025121A1
· 2000
[cited by applicant]
WO WO2000028312
· 2000
[cited by applicant]
WO WO2002024862A2
· 2002
[cited by applicant]
WO WO2002029402A2
· 2002
[cited by applicant]
WO WO2002035221A1
· 2002
[cited by applicant]
WO WO2002082046A2
· 2002
[cited by applicant]
WO WO2003052420A2
· 2003
[cited by applicant]
WO 2004085609A2
· 2004
[cited by applicant]
WO WO2005040783A1
· 2005
[cited by applicant]
WO WO2006012571A1
· 2006
[cited by applicant]
WO WO2006076703A2
· 2006
[cited by applicant]
WO WO2006100484
· 2006
[cited by applicant]
WO WO2006104639
· 2006
[cited by applicant]
WO WO2006113550
· 2006
[cited by applicant]
WO WO2006138160A2
· 2006
[cited by applicant]
WO WO2007028003A2
· 2007
[cited by applicant]
WO WO2007116978A1
· 2007
[cited by applicant]
WO WO2007127327
· 2007
[cited by applicant]
WO WO2007132002A1
· 2007
[cited by applicant]
WO 2007145748A2
· 2007
[cited by applicant]
WO WO2008012552A1
· 2008
[cited by applicant]
WO WO2008054611A2
· 2008
[cited by applicant]
WO WO2008102120
· 2008
[cited by applicant]
WO WO2008102121
· 2008
[cited by applicant]
WO 2008124107
· 2008
[cited by applicant]
WO 2008124107A1
· 2008
[cited by applicant]
WO 2009020682
· 2009
[cited by applicant]
WO WO2009024775A1
· 2009
[cited by applicant]
WO WO2009035647A1
· 2009
[cited by applicant]
WO WO2009077734A2
· 2009
[cited by applicant]
WO WO2010122293
· 2010
[cited by applicant]
WO WO2011154114A2
· 2011
[cited by applicant]
WO WO2012033524A2
· 2012
[cited by applicant]
WO WO2013041878A1
· 2013
[cited by applicant]
WO WO2013057495A2
· 2013
[cited by applicant]
WO WO2013121224A1
· 2013
[cited by applicant]
WO WO2013153359A1
· 2013
[cited by applicant]
WO WO2014064443A2
· 2014
[cited by applicant]
WO WO2014064444A1
· 2014
[cited by applicant]
Y. Kim, Nanopore sensor for fast label-free detection of short double-stranded DNAs, Biosensors and Bioelectronics, 2007, vol. 22, pp. 2926-2931. (Year: 2007).
[cited by examiner]
Cheng, Y., et al. “Discrete membrane arrays.”
[cited by applicant]
Bouaidat, Salim, et al. “Surface-directed capillary system; theory, experiments and applications.”
[cited by applicant]
Bull, Randy A., Fu-Ren F. Fan, and Allen J. Bard. “Polymer Films on Electrodes VII. Electrochemical Behavior at Polypyrrole-Coated Platinum and Tantalum Electrodes.”
[cited by applicant]
Ikariyama, Yoshihito, and William R. Heineman. “Polypyrrole electrode as a detector for electroinactive anions by flow injection analysis.”
[cited by applicant]
Malmstadt, Noah, et al. “Automated formation of lipid-bilayer membranes in a microfluidic device.”
[cited by applicant]
Third Party Submission Under 37 CFR 1.290 for U.S. Appl. No. 14/302,287 dated May 19, 2016.
[cited by applicant]
http://www.cnki.net, China Academic Journal Electronic Publishing House, pp. 275-278 (1986).
[cited by applicant]
[No Author Listed] Avanti Polar Lipids, Inc. Avanti Polar Lipids-Preparations of Liposomes. Www.avantilipids.com 5 pages. Jul. 1, 2014.
[cited by applicant]
Aghdaei et al., Formation of artificial lipid bilayers using droplet dielectrophoresis. Lab Chip. Oct. 2008;8(10):1617-20. doi: 10.1039/b807374k. Epub Aug. 13, 2008.
[cited by applicant]
Altschul et al., Basic local alignment search tool. J Mol Biol. Oct. 5, 1990;215(3):403-10.
[cited by applicant]
Altschul. A protein alignment scoring system sensitive at all evolutionary distances. J Mol Evol. Mar. 1993;36(3):290-300.
[cited by applicant]
Anrather et al., Supported membrane nanodevices. J Nanosci Nanotechnol. Jan.-Feb. 2004;4(1-2):1-22.
[cited by applicant]
Astier et al., Toward single molecule DNA sequencing: direct identification of ribonucleoside and deoxyribonucleoside 5′-monophosphates by using an engineered protein nanopore equipped with a molecular adapter. J Am Che…
[cited by applicant]
Baaken et al., Planar microelectrode-cavity array for high-resolution and parallel electrical recording of membrane ionic currents. Lab Chip. Jun. 2008;8(6):938-44. doi: 10.1039/b800431e. Epub Apr. 16, 2008.
[cited by applicant]
Bezrukov et al., Counting polymers moving through a single ion channel. Nature, Jul. 28, 1994:370(6487):279-81.
[cited by applicant]
Bruggemann et al., Microchip technology for automated and parallel patch-clamp recording. Small. Jul. 2006:2(7):840-6.
[cited by applicant]
Cheng et al., Discrete membrane arrays. J Biotechnol. Sep. 2000;74(3):159-74.
[cited by applicant]
Cheng et al., Single Ion Channel Sensitivity in Suspended Bilayers on Micromachined Supports. Langmuir. 2001:17(4):1240-1242.
[cited by applicant]
Danelon et al., Cell membranes suspended across nanoaperture arrays. Langmuir. Jan. 3, 2006;22(1):22-5.
[cited by applicant]
Devereux et al., A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. Jan. 11, 1984;12(1 Pt 1):387-95.
[cited by applicant]
Estes et al., Electroformation of giant liposomes from spin-coated films of lipids. Colloids Surf B Biointerfaces. May 10, 2005:42(2):115-23.
[cited by applicant]
Funakoshi et al., Lipid bilayer formation by contacting monolayers in a microfluidic device for membrane protein analysis. Anal Chem. Dec. 15, 2006;78(24):8169-74.
[cited by applicant]
Garstecki et al., Formation of droplets and bubbles in a microfluidie T-junction-scaling and mechanism of break-up. Lab Chip. Mar. 2006;6(3):437-46. Epub Jan. 25, 2006. Erratum in: Lab Chip. May 2006:6(5):693.
[cited by applicant]
Hasanzadeh et al., Room-temperature ionic liquid-based electrochemical nanobiosensors. Trends Anal Chem. Dec. 2012;41:58-74.
[cited by applicant]
Heron et al., Simultaneous measurement of ionic current and fluorescence from single protein pores. J Am Chem Soc. Feb. 11, 2009;131(5):1652-3. doi: 10.1021/ja808128s.
[cited by applicant]
Hirano et al., Lipid Bilayers at Gel/Gel Interface for Ion Channel Recordings. Surf. Sci. Nanotech. 2008:6:130-133.
[cited by applicant]
Holden et al., Functional bionetworks from nanoliter water droplets. J Am Chem Soc. Jul. 11, 2007:129(27):8650-5. Epub Jun. 16, 2007.
[cited by applicant]
Hovis et al., Patterning and Composition Arrays of Supported Lipid Bilayers by Microcontact Printing. Langmuir. 2001;17:3400-3405.
[cited by applicant]
Hromada et al., Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip. Lab Chip. Apr. 2008:8(4):602-8. doi:10.1039/b716388f. Epub Feb. 29, 2008.
[cited by applicant]
Ide et al., A novel method for artificial lipid-bilayer formation. Biosens Bioelectron. Oct. 15, 2005;21(4):672-7. Epub Jan. 26, 2005.
[cited by applicant]
Jeon et al., Long-term storable and shippable lipid bilayer membrane platform. Lab Chip. Oct. 2008:8(10):1742-4. doi: 10.1039/6807932c. Epub Aug. 22, 2008.
[cited by applicant]
Jung et al., Detecting protein-ligand binding on supported bilayers by local pH modulation. J Am Chem Soc. Jan. 28, 2009;131(3):1006-14. doi: 10.1021/ja804542p.
[cited by applicant]
Kam et al., Spatially Selective Manipulation of Supported Lipid Bilayers by Laminar Flow: Steps Toward Biomembrane Microfluidic. Langmuir. 2003;19(5):1624-1631.
[cited by applicant]
Kasianowicz et al., Protonation dynamics of the alpha-toxin ion channel from spectral analysis of pH-dependent current fluctuations. Biophys J. Jul. 1995;69(1):94-105.
[cited by applicant]
Khafizov, Single Molecule Force Spectroscopy Of Single Stranded Dna Binding Protein And Rep Helicase. University of Illinois at Urbana-Champaign Dissertation. 2012.
[cited by applicant]
Kim et al., Liquid-slate fiekl-effect transistors using electrowetting. Applied Physics Letters. 90:043507-1-043507-3.
[cited by applicant]
Korolev et al., Major domain swiveling revealed by the crystal structures of complexes of
[cited by applicant]
Krantz Lab. Planar Lip Bilayer Electrohpysiology Equipment. Department of Molecular & Cell Biology, University of California, Berkeley, Oct. 6, 2007. Last accessed at meb.berkeley.edu/labs/krantz/equipment/blm.html on N…
[cited by applicant]
Kung et al., Printing via Photolithography on Micropartitioned Fluid Lipid Membranes. Adv. Materials. 2000;12(10):731-734.
[cited by applicant]
Langecker et al., Synthetic lipid membrane channels formed by designed DNA nanostructures. Science, Nov. 16, 2012;338(6109):932-6. doi: 10.1126/science.1225624.
[cited by applicant]
Le Pioufle et al., Lipid bilayer microarray for parallel recording of transmembrane ion currents. Anal Chem, Jan. 1, 2008;80(1):328-32. Epub Nov. 15, 2007.
[cited by applicant]
Lee et al., Ion channel switch array:A biosensor for detecting multiple pathogens. Industrial Biotechnology, May 2005;1(1):26-31. doi:10.1089/ind.2005.1.26.
[cited by applicant]
Lee et al., Nanoarrays of tethered lipid bilayer rafts on poly(vinyl alcohol) hydrogels. Lab Chip. Jan. 7, 2009;9(1):132-9. doi: 10.1039/b809732a. Epub Oct. 22, 2008.
[cited by applicant]
Lee et al., Polyelectrolyte Micropatterning Using Agarose Plane Stamp and a Substrate Having Microscale Features on Its Surface. Bull. Korean Chem. Soc., vol. 26(10):1539-1542 (2005).
[cited by applicant]
Lewis et al.. The Mesomorphic Phase Behavior of Lipid Bilayers. Structure Biological Membranes. 3rd Ed. Ed: Yeagle, CRC Press 2011. 19-89.
[cited by applicant]
Li et al., Microfluidic system for planar patch clamp electrode arrays. Nano Lett. Apr. 2006;6(4):815-9.
[cited by applicant]
Lieberman et al., Processive replication of single DNA molecules in a nanopore catalyzed by phi29 DNA polymerase. J Am Chem Soc. Dec. 22, 2010;132(50):17961-72. doi:10.1021/ja1087612. Epub Dec. 1, 2010.
[cited by applicant]
Luan et al., Base-by-base ratcheting of single stranded DNA through a solid-state nanopore.Phys Rev Lett. Jun. 11, 2010:104(23):238103. Epub Jun. 10, 2010.
[cited by applicant]
Mach et al., Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion. Anal Bioanal Chem. Feb. 2008:390(3):841-6. Epub Oct. 31, 2007.
[cited by applicant]
Majd et al., Hydrogel stamping of arrays of supported lipid bilayers with various lipid compositions for the screening of drug-membrane and protein-membrane interactions. Angew Chem Int Ed Engl. Oct. 21, 2005;44(41):669…
[cited by applicant]
Malmstadt et al., Automated formation of lipid-bilayer membranes in a microfluidic device. Nano Lett. Sep. 2006;6(9):1961-5.
[cited by applicant]
Mangold et al., Reference electrodes based on conducting polymers. Fresenius J Anal Chem. Jun. 2000:367(4):340-2.
[cited by applicant]
Mastrangeli et al., Challenges for Capillary Self-Assembly of Microsystems. IEEE Transactions. Jan. 2011;1(1):133-149.
[cited by applicant]
Mastrangeli et al., Self-assembly from milli- to nanoscales:methods and applications. J Micro Microeng. 2009;19:083001.
[cited by applicant]
Maurer et al., Reconstitution of ion channels in agarose-supported silicon orifices. Biosens Bioelectron. May 15, 2007:22(11):2577-84. Epub Nov. 13, 2006.
[cited by applicant]
McAlduff et al., Freestanding lipid bilayers as substrates for electron cryomicroscopy of integral membrane proteins. J Microsc. Feb. 2002;205(Pt 2):113-7.
[cited by applicant]
Montal et al., Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci U S A. Dec. 1972;69(12):3561-6.
[cited by applicant]
Moran-Mirabal et al., Micrometer-sized supported lipid bilayer arrays for bacterial toxin binding studies through total internal reflection fluorescence microscopy. Biophys J. Jul. 2005;89(1):296-305. Epub Apr. 15, 2005.
[cited by applicant]
Ogier et al., “Suspended Planar Phospholipid Bilayers on Micromachined Supports,” Langomir, vol. 16:5696-5701 (2000).
[cited by applicant]
Onoe et al., Three-Dimensional Micro-Self-Assembly Using Hydrophobic Interaction Controlled by Self-Assembled Monolayers. J Micro Systems. Aug. 2004;13(4):603-611.
[cited by applicant]
Parthasarathy et al., Protein patterns at lipid bilayer junctions, Proc Natl Acad Sci U S A. Aug. 31, 2004:101(35):12798-803. Epub Aug. 20, 2004.
[cited by applicant]
Peterman et al., “Ion Channels and Lipid Bilayer Membranes Under High Potentials Using Microfabricaled Apertures,” Biomedical Microdevices, vol. 4(3):231-236 (2002).
[cited by applicant]
Polk et al., “Ag/AgCl microelectrodes with improved stability for microfluidics.” Sensors and Actuators B., vol. 114:239-247 (2006).
[cited by applicant]
Rauf et al., Studies on sildenafil citrate (Viagra) interaction with DNA using electrochemical DNA biosensor. Biosens Bioelectron. May 15, 2007:22(11):2471-7. Epub Nov. 7, 2006.
[cited by applicant]
Romer et al.. Impedance analysis and single-channel recordings on nano-black lipid membranes based on porous alumina. Biophys J. Feb. 2004;86(2):955-65.
[cited by applicant]
Sackmann, Supported membranes: scientific and practical applications. Science. Jan. 5, 1996;271(5245):43-8.
[cited by applicant]
Sandison et al., “Rapid fabrication of polymer microftuidic systems for the production of artificial lipid bilayers,” J. Micromelh. Microeng., vol. 15:S139-S144 (2005).
[cited by applicant]
Sandison et al., Air-exposure technique for the formation of artificial lipid bilayers in microsystems. Langmuir. Jul. 17, 2007:23(15):8277-84. Epub Jun. 22, 2007.
[cited by applicant]
Sapra et al., Lipid-coated hydrogel shapes as components of electrical circuits and mechanical devices. Sci Rep. 2012;2:848. doi: 10.1038/srep00848. Epub Nov. 14, 2012.
[cited by applicant]
Sarles et al., Bilayer formation between lipid-encased hydrogels contained in solid substrates. ACS Appl Mater Interfaces. Dec. 2010:2(12):3654-63. doi: 10.1021/am100826s. Epub Nov. 10, 2010.
[cited by applicant]
Schindler et al., Branched bimolecular lipid membranes. Biophys J. Sep. 1976;16(9):1109-13.
[cited by applicant]
Schmidt et al., A Chip-Based Biosensor for the Functional Analysis of Single Ion Channels. Angew Chem Int Ed Engl. Sep. 1, 2000;39(17):3137-3140.
[cited by applicant]
Shim et al., Stochastic sensing on a modular chip containing a single-ion channel. Anal Chem. Mar. 15, 2007;79(6):2207-13. Epub Feb. 9, 2007.
[cited by applicant]
Soni et al., Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores. Rev Sci Instrum. Jan. 2010:81(1):014301. doi: 10.1063/1.3277116.
[cited by applicant]
Stoddart et al., Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore. Proc Natl Acad Sci U S A. May 12, 2009;106(19):7702-7. doi: 10.1073/pnas.0901054106. Epub Apr. 20, 2009.
[cited by applicant]
Sun et al., Microfluidic static droplet arrays with tuneable gradients in material composition. Lab Chip. Dec. 7, 2011;11(23):3949-52. doi: 10.1039/ellc20709a. Epub Oct. 12, 2011.
[cited by applicant]
Suzuki et al., Highly reproducible method of planar lipid bilayer reconstitution in polymethyl methacrylate microfluidic chip. Langmuir. Feb. 14, 2006;22(4):1937-42.
[cited by applicant]
Suzuki et al., Planar lipid bilayer reconstitution with a micro-fluidie system. Lab Chip. Oct. 2004;4(5):502-5. Epub Sep. 2, 2004.
[cited by applicant]
Suzuki et al., Planar Lipid Membrane Array for Membrane Protein Chip. 17th IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 272-275 (2004).
[cited by applicant]
Syms et al., Surface Tension-Powered Self-Assembly of Microstructures—The State of the Art. J Micro Systems. Aug. 2003;12(4):387-417.
[cited by applicant]
Thorsen et al., Dynamic pattern formation in a vesicle-generating microfluidic device. Phys Rev Lett. Apr. 30, 2001;86(18):4163-6.
[cited by applicant]
Urisu et al., Formation of high-resistance supported lipid bilayer on the surface of a silicon substrate with microelectrodes. Nanomedicine. Dec. 2005;1(4):317-22.
[cited by applicant]
Vidinha et al., Ion jelly: a tailor-made conducting material for smart electrochemical devices. Chem Commun (Camb). Nov. 30, 2008;(44):5842-4. doi: 10.1039/b811647d. Epub Oct. 3, 2008.
[cited by applicant]
Vulto et al., Microfluidic channel fabrication in dry film resist for production and prototyping of hybrid chips. Lab Chip. Feb. 2005;5(2):158-62. Epub Dec. 3, 2004.
[cited by applicant]
Wagterveld et al., Ultralow hysteresis superhydrophobic surfaces by excimer laser modification of SU-8. Langmuir. Dec. 19, 2006;22(26):10904-8.
[cited by applicant]
Zagnoni et al., Bilayer lipid membranes from falling droplets. Anal Bioanal Chem. Mar. 2009;393(6-7):1601-5. doi:10.1007/s00216-008-2588-5. Epub Jan. 19, 2009.
[cited by applicant]
Zagnoni et al., Controlled delivery of proteins into bilayer lipid membranes on chip. Lab Chip. Sep. 2007;7(9):1176-83. Epub Jun. 27, 2007.
[cited by applicant]
Zagnoni et al., Microfluidic array platform for simultaneous lipid bilayer membrane formation. Biosens Bioelectron. Jan. 1, 2009;24(5):1235-40. doi: 10.1016/j.bios.2008.07.022. Epub Jul. 23, 2008.
[cited by applicant]
Hui Wang et al., “Nanopores with a spark for single-molecule detection”, at 2001 Nature Publishing Group, nature biotechnology vol. 19, Jul. 2001 pp. 622-623.
[cited by applicant]
William L. Hwang et al., “Electrical Behavior of Droplet Interface Bilayer Networks: Experimental Analysis and Modeling”, JACS Articles, J. Am. Chem. Soc. 2007, 129, p. 11854-11864, Sep. 1, 2007.
[cited by applicant]
Michael Goryll et al., “Integrated sensor design using ion channels inserted into lipid bilayer membranes”, 2004 4
[cited by applicant]
Hagan Bayley et al., “Stochastic sensors inspired by biology”, Nature, vol. 413, Sep. 13, 2001, pp. 226-230.
[cited by applicant]
Jacob Schmidt, “Stochastic sensors”, J. Mater. Chem., 2005, 15, 831-840, DOI: 10.1039/b414551h.
[cited by applicant]
S.J. Wilk et al., “Integrated electrodes on a silicon based ion channel measurement platform”, Biosensors and Bioelectronics 23 (Apr. 8, 2007) 183-190.
[cited by applicant]
Stefan Howorka et al., “Sequence-specific detection of individual DNA strands using engineered nanopores”, Nature Biotechnology vol. Jul. 19, 2001 pp. 636-639.
[cited by applicant]