Granted Patent
B2
US 12,235,260 · App. 18/394,975 · Granted Feb 25, 2025
Nanopore-based analysis of analytes
Inventors:
Giovanni Maglia (Gilmmen, NL); Shengli Zhang (Shanghai, CN); Andrew Heron (Maidenhead, GB)
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Abstract
The present disclosure relates to systems and methods for analysis of proteins, more in particular to nanopore systems, devices and methods for single-molecule protein analysis and sequencing. Provided is a method for translocating a target protein through a nanopore, the nanopore being comprised in a membrane separating a fluidic chamber of a nanopore system into a cis side and a trans side, comprising: (a) allowing a protein translocase in solution to capture and form a complex with the target protein to be translocated; (b) contacting the translocase-target protein complex with the cis side of the nanopore and allowing for translocation of the target protein to the trans side; wherein the nanopore system has a cis to trans electro-osmotic force (EOF) resulting from a large net ionic current flow cis-to-trans relative to the total ionic current flow, so that the target protein is captured in the nanopore with on top of the nanopore the translocase controlling the translocation.
Claims (24)
1. A method comprising:
(a) providing:
(i) a nanopore system, wherein the nanopore system comprises (1) a fluidic chamber, and (2) a membrane comprising a nanopore, wherein the membrane separates the fluidic chamber into a cis side and a trans side;
(b) contacting (1) a non-nucleic acid based polymer analyte, and (2) a translocase with the cis side of the nanopore; and
(c) translocating the non-nucleic acid based polymer analyte to the trans side of the fluidic chamber using an electro-osmotic force, wherein the electro-osmotic force acts from the cis side to the trans side, wherein the electro-osmotic force maintains the translocase at an entrance of a channel of the nanopore on the cis side of the fluidic chamber.
2. The method of claim 1 , further comprising, prior to (b), contacting the non-nucleic acid based polymer analyte with the translocase to generate a complex.
3. The method of claim 1 , wherein the electro-osmotic force is modulated by a pH, a type of a salt, a concentration of a salt, an osmotic pressure across the membrane, or a modification of the nanopore, or any combination thereof.
4. The method of claim 3 , wherein the modification of the nanopore comprises a modification of a charge of the nanopore.
5. The method of claim 1 , wherein the electro-osmotic force is modulated by an asymmetric salt distribution between the cis side and the trans side of the fluidic chamber.
6. The method of claim 1 , wherein the translocase comprises an Adenosine triphosphate (ATP)-driven unfoldase.
7. The method of claim 1 , wherein the translocase comprises a Nucleotide triphosphate (NTP)-driven unfoldase.
8. The method of claim 1 , wherein the translocase comprises an AAA+ enzyme.
9. The method of claim 1 , wherein the nanopore system further comprises a pair of electrodes that provides an applied voltage to generate an electrophoretic force.
10. The method of claim 9 , wherein a magnitude of the applied voltage is less than 1000 millivolts (mVs).
11. The method of claim 9 , wherein an absolute relative net electro-osmotic current over the applied voltage is greater than about 0.10 picoampere/millivolt (pA/mV).
12. The method of claim 1 , wherein the nanopore comprises an ion-selectivity P(+)/P(−) of greater than 2.0.
13. The method of claim 1 , wherein the nanopore comprises an ion-selectivity P(+)/P(−) of less than 0.50.
14. The method of claim 1 , wherein the non-nucleic acid based polymer analyte is an unmodified non-nucleic acid based polymer analyte.
15. The method of claim 1 , wherein the non-nucleic acid based polymer analyte comprises peptide units, saccharide units, or water-soluble plastic monomers, or any combination thereof.
16. The method of claim 1 , further comprising measuring a signal generated by the translocating of the non-nucleic acid based polymer analyte through the nanopore.
17. The method of claim 16 , wherein the measuring comprises measuring the signal for states of (i) an open channel of the nanopore; (ii) capture of the non-nucleic acid based polymer analyte by the nanopore; or (iii) passage of the non-nucleic acid based polymer analyte through the nanopore.
18. The method of claim 1 , wherein the nanopore comprises a biological nanopore.
19. The method of claim 1 , wherein the nanopore comprises an inner pore constriction from about 0.5 nanometers (nm) to about 2 nm.
20. The method of claim 1 , wherein the nanopore comprises Aerolysin (Aer), Cytolysin K (CytK), Mycobacterium smegmatis porin A (MspA), alpha-hemolysin (aHL), CsgG, Fragaceatoxin C (FraC), Lysenin, outer membrane porin F (OmpF), outer membrane porin G (OmpG), FhuA, or phage derived portal proteins, or modified variants thereof, or ion-selective mutants thereof, or any combination thereof.
Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 6, 2024
From: HERON, ANDREW
To: PORTAL BIOTECH LIMITED
Reel/Frame 069514/0161 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 6, 2024
From: ZHANG, SHENGLI
To: RIJKSUNIVERSITEIT GRONINGEN
Reel/Frame 069514/0184 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 6, 2024
From: MAGLIA, GIOVANNI
To: RIJKSUNIVERSITEIT GRONINGEN
Reel/Frame 069514/0204 →
Priority Claims (1)
EP 22204590
· Oct 28, 2022
· regional
Continuity (2)
Continuation
PCTNL2023050570
· Oct 30, 2023
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