Granted Patent
B2
US 12,259,325 · App. 18/397,463 · Granted Mar 25, 2025
Zinc-responsive fluorophores
Inventors:
Seth A. Garwin (Evanston, IL); Emily L. Que (Evanston, IL); Thomas V. O'Halloran (Evanston, IL); Teresa K. Woodruff (Chicago, IL)
Assignee:
Northwestern University
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Abstract
The invention relates generally to compositions and methods for the detection of zinc. Provided herein is a class of zinc-responsive probes with tunable photophysical properties that can be modified for coupling to a solid support or other chemical moieties. In particular, modifications to the 5-position of the BODIPY core allows for alteration of probe properties and functionalities.
Claims (8)
1. A method comprising:
(a) contacting a sample with zinc-responsive probe comprising:
and
(b) detecting light emitted from the zinc-responsive probe.
2. The method of claim 1 , wherein detecting light emitted from the zinc-responsive probe comprises (i) exposing the sample to a wavelength of light within an excitation spectrum of the zinc-responsive probe, and (ii) detecting light within an emission spectrum of the zinc-responsive probe.
3. The method of claim 1 , wherein detecting light from the zinc-responsive probe comprises monitoring light emitted the zinc-responsive probe over time.
4. The method of claim 1 , wherein detecting an emission spectrum of the zinc-responsive probe comprises imaging the sample.
5. The method of claim 1 , wherein the sample comprises a cell, tissue, organ, or whole animal.
Assignments (2)
CONFIRMATORY LICENSE
Recorded Feb 1, 2024
From: NORTHWESTERN UNIVERSITY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 066407/0146 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 10, 2024
From: GARWIN, SETH A; QUE, EMILY L; O'HALLORAN, THOMAS V; WOODRUFF, TERESA K.
To: NORTHWESTERN UNIVERSITY
Reel/Frame 066081/0120 →
Continuity (3)
Provisional Application
62907268
· Sep 27, 2019
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