FUSION PROTEINS AND METHODS THEREOF
The invention discloses oncogenic fusion proteins. The invention provides methods for treating gene-fusion based cancers.
1 .- 23 . (canceled)
24 . A kit for amplifying a cDNA encoding a FGFR1-TACC1 fusion protein or a complement thereof, or a FGFR3-TACC3 fusion protein or a complement thereof, the kit comprising:
a synthetic polynucleotide probe comprising a polynucleotide sequence complementary to a portion of the cDNA encoding the FGFR1-TACC1 fusion protein or the complement thereof, or a portion of the cDNA encoding the FGFR3-TACC3 fusion protein or the complement thereof,
wherein the synthetic polynucleotide probe further comprises a chemical modification,
wherein the chemical modification comprises a radioactive molecule, a fluorescent molecule, a chemiluminescent molecule, an enzyme, or an affinity molecule, and
wherein the kit is configured to allow for amplification of the cDNA encoding the FGFR1-TACC1 fusion protein or the complement thereof or the FGFR3-TACC3 fusion protein or the complement thereof.
25 . The kit of claim 24 , wherein the synthetic polynucleotide probe comprises the polynucleotide sequence complementary to the portion of the cDNA encoding the FGFR3-TACC3 fusion protein or the complement thereof.
26 . The kit of claim 25 , wherein the cDNA encoding the FGFR3-TACC3 fusion protein comprises a tyrosine kinase domain of the FGFR3 portion fused to a TACC domain of the TACC3 portion.
27 . The kit of claim 25 , wherein the synthetic polynucleotide probe comprises a polynucleotide sequence complementary to a portion of the cDNA encoding a breakpoint between a FGFR3 portion and a TACC3 portion of a FGFR3-TACC3 fusion protein or the complement thereof.
28 . The kit of claim 27 , wherein the breakpoint between the FGFR3 portion and the TACC3 portion of the FGFR3-TACC3 fusion protein comprises SEQ ID NO: 85, 86, 87, or 89.
29 . The kit of claim 27 , wherein the synthetic polynucleotide probe sequence comprises SEQ ID NO: 80, 81, 82, or 84, or a complement thereof.
30 . The kit of claim 24 , wherein the synthetic polynucleotide probe comprises the polynucleotide sequence complementary to the portion of the cDNA encoding the FGFR1-TACC1 fusion protein or the complement thereof.
31 . The kit of claim 30 , wherein the synthetic probe comprises a polynucleotide sequence complementary to a portion of the cDNA encoding a breakpoint between a FGFR1 portion and a TACC1 portion of the FGFR1-TACC1 fusion protein or the complement thereof.
32 . The kit of claim 31 , wherein the breakpoint between the FGFR1 portion and the TACC1 portion of the FGFR1-TACC1 fusion protein comprises SEQ ID NO: 88.
33 . The kit of claim 31 , wherein the synthetic polynucleotide probe sequence comprises SEQ ID NO: 83, or a complement thereof.
34 . The kit of claim 24 , wherein the synthetic probe is between 10 and 30 nucleotides long.
35 . The kit of claim 24 , wherein the chemical modification comprises a fluorescent molecule.
36 . The kit of claim 35 , wherein the fluorescent molecule is capable of being detected during an amplification reaction.
37 . The kit of claim 36 , wherein the amplification reaction is RT-PCR.