Granted Patent B2

US 12,599,073 · App. 18/426,902 · Granted Apr 14, 2026

Inventors: Jian Lv (Beijing, CN); Kun Yu (Beijing, CN); Juan Wei (Beijing, CN); Chunxia Liu (Beijing, CN); Hongju Zhou (Beijing, CN); Timothy Kelliher (Research Triangle Park, NC)

Assignee: Syngenta Crop Protection AG

A01H6/4678

Abstract

Claims (3)

1 . A method of generating a haploid-inducing wheat plant, the method comprising: a. obtaining at least a wheat plant cell comprising an A genome, a B genome, and a D genome; b. mutating the B genome to obtain a homozygous knock-out mutation in a CENH3α gene; c. mutating the D genome to obtain a homozygous knock-out mutation in a CENH3α gene; d. mutating the A genome to obtain a knock-down mutation at a 5′ splice site of an intron in a CENH3α gene; and e. generating a wheat plant therefrom; whereby the wheat plant generated from step e. produces haploid progeny when crossed with a wildtype wheat plant.

2 . The method of claim 1 , wherein the knock-down mutation in the CENH3α gene is a restored frame shift mutation.

3 . The method of claim 2 , wherein the restored frame shift mutation is selected from the group consisting of SEQ ID NO: 56, a nucleic acid sequence 98% identical to SEQ ID NO: 56, SEQ ID NO: 63, a nucleic acid sequence 98% identical to SEQ ID NO: 63, or a combination thereof.

Assignments (2)

Priority Claims (1)

Continuity (2)

References Cited (24)