Granted Patent
B2
US 12,371,458 · App. 18/440,278 · Granted Jul 29, 2025
Mutant pore
Inventors:
Lakmal Nishantha Jayasinghe (Oxford, GB); Mark John Bruce (Oxford, GB); Luke McNeill (Oxford, GB); Ramiz Iqbal Nathani (Oxford, GB); Pratik Raj Singh (Oxford, GB); Neil Roger Wood (Oxford, GB); Stephen Robert Young (Oxford, GB)
Assignee:
Oxford Nanopore Technologies PLC
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Abstract
The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.
Claims (14)
1. An apparatus comprising a transmembrane protein pore inserted into an in vitro membrane, wherein the transmembrane protein pore comprises at least one mutant lysenin monomer comprising a variant of the sequence set forth in SEQ ID NO: 2, wherein the variant comprises the S49K/R/L amino acid substitution.
2. The apparatus of claim 1 , wherein the transmembrane protein pore is a homo-oligomeric pore comprising the at least one mutant lysenin monomer.
3. The apparatus of claim 1 , wherein the transmembrane protein pore is a hetero-oligomeric pore comprising the at least one mutant lysenin monomer.
4. The apparatus of claim 1 , wherein the variant further comprises the E94 D/Q/G/A/K/R amino acid substitution.
5. An apparatus for characterizing a target analyte, the apparatus comprising a plurality of mutant lysenin monomers, wherein each mutant lysenin monomer comprises a variant of the sequence set forth in SEQ ID NO: 2, wherein the variant comprises the S49K/R/L amino acid substitution, and wherein the plurality of mutant lysenin monomers is inserted into a plurality of in vitro membranes.
6. The apparatus of claim 5 , wherein each of the plurality of in vitro membranes comprises a homo-oligomeric pore comprising the mutant lysenin monomers.
7. The apparatus of claim 5 , wherein each of the plurality of in vitro membranes comprises a hetero-oligomeric pore, and wherein at least one monomer of the hetero-oligomeric pore is the mutant lysenin monomer.
8. The apparatus of claim 5 , wherein each lysenin variant further comprises the E94 D/Q/G/A/K/R amino acid substitution.
9. A transmembrane protein pore comprising at least one mutant lysenin monomer, wherein the at least one mutant lysenin monomer comprises the amino acid sequence of SEQ ID NO: 2 having the S49K/R/L amino acid substitution.
10. The pore of claim 9 , wherein the pore is a hetero-oligomeric pore.
11. The pore of claim 9 , wherein the pore is a homo-oligomeric pore.
12. The pore of claim 9 , wherein the at least one mutant lysenin monomer further comprises the E94 D/Q/G/A/K/R amino acid substitution.
13. An apparatus produced by a method comprising: (i) obtaining a transmembrane protein pore comprising at least one mutant lysenin monomer, wherein the at least one mutant lysenin monomer comprises the amino acid sequence of SEQ ID NO: 2 having the S49K/R/L amino acid substitution and (ii) contacting the pore with an in vitro membrane such that the pore is inserted into the in vitro in vitro membrane.
14. The pore of claim 13 , wherein the at least one mutant lysenin monomer further comprises the E94 D/Q/G/A/K/R amino acid substitution.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 12, 2024
From: JAYASINGHE, LAKMAL; BRUCE, MARK JOHN; MCNEILL, LUKE; NATHANI, RAMIZ IQBAL; SINGH, PRATIK RAJ; WOOD, NEIL ROGER; YOUNG, STEPHEN ROBERT
To: OXFORD NANOPORE TECHNOLOGIES LTD.
Reel/Frame 067972/0184 →
CHANGE OF NAME
Recorded Jul 12, 2024
From: OXFORD NANOPORE TECHNOLOGIES LIMITED
To: OXFORD NANOPORE TECHNOLOGIES PLC
Reel/Frame 068315/0855 →
Priority Claims (2)
GB 1605899
· Apr 6, 2016
· national
GB 1608274
· May 11, 2016
· national
Continuity (3)
Continuation
16091746
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