Granted Patent
B2
US 12,324,431 · App. 18/452,223 · Granted Jun 10, 2025
Compositions and methods for scalable production and delivery of biologicals
Inventors:
Ameer Hamza Shakeel (Leesburg, VA); Zachery George Davis (Charlottesville, VA); Joseph Thomas Frank (Charlottesville, VA); Sepehr Zomorodi (Charlottesville, VA); Payam Pourtaheri (Charlottesville, VA)
Assignee:
AgroSpheres, Inc.
View Patent ↗
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Abstract
The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.
Claims (29)
1. An agricultural composition, comprising:
a) an insecticide and
b) a minicell encapsulating a nucleic acid that is capable of inducing RNA interference in an agricultural target pest.
2. The agricultural composition of claim 1 , wherein the nucleic acid is RNA.
3. The agricultural composition of claim 1 , wherein the nucleic acid is at least one selected from the group consisting of: a double-stranded RNA (dsRNA), a short-hairpin RNA (shRNA), a small-interfering RNA (siRNA), and a microRNA (miRNA).
4. The agricultural composition of claim 1 , wherein the nucleic acid is dsRNA.
5. The agricultural composition of claim 1 , wherein the nucleic acid is shRNA.
6. The agricultural composition of claim 1 , wherein the nucleic acid is siRNA.
7. The agricultural composition of claim 1 , wherein the nucleic acid is miRNA.
8. The agricultural composition of claim 1 , wherein the nucleic acid is capable of inducing RNA interference in at least one agricultural target pest selected from the group consisting of: fungus, insect, nematode, mite, and tick.
9. The agricultural composition of claim 1 , wherein the nucleic acid is capable of inducing RNA interference in an insect.
10. The agricultural composition of claim 1 , wherein the nucleic acid is capable of inducing RNA interference in at least one member from an order selected from the group consisting of: coleoptera, diptera, hymenoptera, lepidoptera, homoptera, hemiptera, orthoptera, thysanoptera, dermaptera, siphonaptera , and trichoptera.
11. The agricultural composition of claim 1 , wherein the nucleic acid is capable of inducing RNA interference in a member of the order lepidoptera.
12. The agricultural composition of claim 1 , wherein the nucleic acid is capable of inducing RNA interference in a member of the genus Plutella.
13. The agricultural composition of claim 1 , wherein the nucleic acid is capable of inducing RNA interference in a member of the genus Spodoptera.
14. The agricultural composition of claim 1 , wherein the minicell is ribonuclease deficient.
15. The agricultural composition of claim 1 , wherein the minicell comprises at least one fusion protein.
16. The agricultural composition of claim 1 , wherein the minicell comprises at least one fusion protein expressed on a surface of the minicell.
17. The agricultural composition of claim 1 , wherein the minicell comprises at least one fusion protein expressed on a surface of the minicell, said fusion protein comprising at least one target cell adhesion moiety.
18. The agricultural composition of claim 1 , wherein the minicell comprises at least one fusion protein expressed on a surface of the minicell, said fusion protein comprising a carbohydrate binding molecule.
19. The agricultural composition of claim 1 , wherein the insecticide is cyantraniliprole.
20. The agricultural composition of claim 1 , wherein the insecticide is chlorantraniliprole.
21. The agricultural composition of claim 1 ,
wherein the insecticide is cyantraniliprole and/or chlorantraniliprole, and wherein the nucleic acid is capable of inducing RNA interference in a member of the genus Plutella and/or Spodoptera.
22. An agricultural composition, comprising:
a minicell encapsulating a nucleic acid that is capable of inducing RNA interference in a member of the order lepidoptera.
23. The agricultural composition of claim 22 , wherein the nucleic acid is capable of inducing RNA interference in a member of the genus Plutella.
24. The agricultural composition of claim 22 ,
wherein the nucleic acid is capable of inducing RNA interference in a member of the genus Spodoptera.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 25, 2023
From: SHAKEEL, AMEER HAMZA; DAVIS, ZACHERY GEORGE; FRANK, JOSEPH THOMAS; ZOMORODI, SEPEHR; POURTAHERI, PAYAM
To: AGROSPHERES, INC.
Reel/Frame 064701/0664 →
Continuity (4)
Continuation
16649857
Provisional Application
62562723
· Sep 25, 2017
Provisional Application
62666981
· May 4, 2018
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