Compositions and methods for the encapsulation and scalable delivery of agrochemicals
The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.
1. A minicell composition, comprising:
a) a minicell having encapsulated within said minicell an agrochemical.
2. The minicell composition according to claim 1 , further comprising:
b) at least one agriculturally acceptable carrier.
3. The minicell composition according to claim 1 , wherein the minicell is derived from a prokaryotic cell.
4. The minicell composition according to claim 1 , wherein the minicell is derived from a gram negative bacterial genus.
5. The minicell composition according to claim 1 , wherein the minicell is derived from a bacterial genus selected from the group consisting of: Escherichia, Salmonella, Shigella, Pseudomonas , and Agrobacterium.
6. The minicell composition according to claim 1 , wherein the minicell is derived from a bacterial species selected from the group consisting of: Escherichia coli, Salmonella typhimurium, Shigella flexneri , and Pseudomonas aeruginosa.
7. The minicell composition according to claim 1 , wherein the minicell is derived from a gram positive bacterial genus.
8. The minicell composition according to claim 1 , wherein the minicell is derived from a bacterial genus selected from the group consisting of: Bacillus, Corynebacterium , and Lactobacillus.
9. The minicell composition according to claim 1 , wherein the minicell is derived from a bacterial species selected from the group consisting of: Bacillus subtilis, Corynebacterium glutamicum , and Lactobacillus acidophilus.
10. The minicell composition according to claim 1 , wherein the minicell is derived from a parental bacterial cell that has one or more genomic deletions.
11. The minicell composition according to claim 1 , wherein the minicell is derived from a parental bacterial cell that has one or more gene deletions.
12. The minicell composition according to claim 1 , wherein the minicell is derived from a parental bacterial cell that has a mutated min gene.
13. The minicell composition according to claim 1 , wherein the minicell is derived from a parental bacterial cell that has a mutated minC, minD, and/or minE gene.
14. The minicell composition according to claim 1 , wherein the minicell is derived from a parental bacterial cell deficient in one or more proteases.
15. The minicell composition according to claim 1 , wherein the minicell is derived from a parental bacterial cell that has one or more genomic deletions and a mutated minC, minD, and/or minE gene.
16. The minicell composition according to claim 1 , formulated as a liquid, dry composition, powder, granule, seed coating, drench, in-furrow composition, or foliar spray.
17. The minicell composition according to claim 1 , wherein the minicell exhibits a controlled release rate of the agrochemical.
18. The minicell composition according to claim 1 , wherein the minicell exhibits a steady release rate of the agrochemical.
19. The minicell composition according to claim 1 , wherein the minicell exhibits a burst release rate of the agrochemical.
20. A method of delivering an agrochemical to a locus, comprising: applying the minicell composition according to claim 1 to a locus.
21. A method of delivering an agrochemical to a plant, comprising: applying the minicell composition according to claim 1 to a plant.