Mutated glycoprotein of vesicular stomatitis virus
The invention relates to an isolated non-naturally occurring protein comprising the amino acid sequence as set forth in SEQ ID NO: 1, and wherein the amino acid in position 8, 47, 209 and/or 354 is substituted by any amino acid different from the amino acid indicated at that position in said sequence SEQ ID NO: 1.
1. A polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises an alanine or glutamine substitution at position 47 and an alanine or glutamine substitution at position 354.
2. The polypeptide of claim 1 , wherein the polypeptide comprises an amino acid sequence at least 96% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises an alanine or glutamine substitution at position 47 and an alanine or glutamine substitution at position 354.
3. The polypeptide of claim 1 , wherein the polypeptide comprises an amino acid sequence at least 97% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises an alanine or glutamine substitution at position 47 and an alanine or glutamine substitution at position 354.
4. The polypeptide of claim 1 , wherein the polypeptide comprises an amino acid sequence at least 98% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises an alanine or glutamine substitution at position 47 and an alanine or glutamine substitution at position 354.
5. The polypeptide of claim 1 , wherein the polypeptide comprises an amino acid sequence at least 99% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises an alanine or glutamine substitution at position 47 and an alanine or glutamine substitution at position 354.
6. The polypeptide of claim 1 , wherein the polypeptide comprises:
(a) an alanine substitution at position 47 and position 354;
(b) an alanine substitution at position 47 and a glutamine substitution at position 354;
(c) a glutamine substitution at position 47 and position 354; or
(d) a glutamine substitution at position 47 and an alanine substitution at position 354.
7. The polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence set forth in any one of SEQ ID NOs: 163, 166, 175, and 178.
8. A nucleic acid molecule encoding the polypeptide of claim 1 .
9. A nucleic acid molecule encoding the polypeptide of claim 5 .
10. A nucleic acid molecule encoding the polypeptide of claim 6 .
11. A nucleic acid molecule encoding the polypeptide of claim 7 .
12. A recombinant virus expressing the polypeptide of claim 1 .
13. A recombinant virus expressing the polypeptide of claim 5 .
14. A recombinant virus expressing the polypeptide of claim 6 .
15. A recombinant virus expressing the polypeptide of claim 7 .
16. A polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises a glutamine substitution at position 47 or an alanine or glutamine substitution at position 354.
17. The polypeptide of claim 16 , wherein the polypeptide comprises an amino acid sequence at least 96% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises a glutamine substitution at position 47 or an alanine or glutamine substitution at position 354.
18. The polypeptide of claim 16 , wherein the polypeptide comprises an amino acid sequence at least 97% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises a glutamine substitution at position 47 or an alanine or glutamine substitution at position 354.
19. The polypeptide of claim 16 , wherein the polypeptide comprises an amino acid sequence at least 98% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises a glutamine substitution at position 47 or an alanine or glutamine substitution at position 354.
20. The polypeptide of claim 16 , wherein the polypeptide comprises an amino acid sequence at least 99% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the polypeptide comprises a glutamine substitution at position 47 or an alanine or glutamine substitution at position 354.
21. The polypeptide of claim 16 , wherein the polypeptide comprises:
(a) a glutamine substitution at position 47;
(b) an alanine substitution at position 354; or
(c) a glutamine substitution at position 354.
22. The polypeptide of claim 16 , wherein the polypeptide comprises the amino acid sequence set forth in any one of SEQ ID NOs: 158, 159, and 162.
23. A nucleic acid molecule encoding the polypeptide of claim 16 .
24. A nucleic acid molecule encoding the polypeptide of claim 20 .
25. A nucleic acid molecule encoding the polypeptide of claim 21 .
26. A nucleic acid molecule encoding the polypeptide of claim 22 .
27. A recombinant virus expressing the polypeptide of claim 16 .
28. A recombinant virus expressing the polypeptide of claim 20 .
29. A recombinant virus expressing the polypeptide of claim 21 .
30. A recombinant virus expressing the polypeptide of claim 22 .